Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed
Background and Aim: Heat shock proteins are highly conserved proteins that work as molecular chaperones expressed in response to thermal stress. This study aimed to determine the expression profile of genes related to the heat stress response in whole blood obtained from the Romosinuano creole breed...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-03-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.16/March-2023/22.pdf |
| _version_ | 1797860738315845632 |
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| author | Juan Camilo Taborda-Charris Roy Rodríguez-Hernández María Paula Herrera-Sánchez Heinner Fabian Uribe-García Rafael J. Otero-Arroyo Juan Sebastian Naranjo-Gomez Kelly Johanna Lozano-Villegas Iang Schroniltgen Rondón-Barragín |
| author_facet | Juan Camilo Taborda-Charris Roy Rodríguez-Hernández María Paula Herrera-Sánchez Heinner Fabian Uribe-García Rafael J. Otero-Arroyo Juan Sebastian Naranjo-Gomez Kelly Johanna Lozano-Villegas Iang Schroniltgen Rondón-Barragín |
| author_sort | Juan Camilo Taborda-Charris |
| collection | DOAJ |
| description | Background and Aim: Heat shock proteins are highly conserved proteins that work as molecular chaperones expressed in response to thermal stress. This study aimed to determine the expression profile of genes related to the heat stress response in whole blood obtained from the Romosinuano creole breed.
Materials and Methods: Real-time polymerase chain reaction was performed to analyze the transcript of hsp90, hsp70, hsp60, and hsf1 in the whole blood of Romosinuano under different temperature-humidity indices (THIs).
Results: The expression levels of the hsp70 and hsf1 genes at the high-THI level were higher (p = 0.0011 and p = 0.0003, respectively) than those at the low-THI level. In addition, no differences in the expression levels of the hsp60 and hsP90 genes were detected between the two THIs.
Conclusion: The overexpression of hsf1 and hsp70 genes play an important role in protecting cells from damage induced by heat stress. |
| first_indexed | 2024-04-09T21:51:46Z |
| format | Article |
| id | doaj.art-2eee3b0544ea43e092f8ef6796710eba |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-09T21:51:46Z |
| publishDate | 2023-03-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-2eee3b0544ea43e092f8ef6796710eba2023-03-24T15:36:07ZengVeterinary WorldVeterinary World0972-89882231-09162023-03-0116360160610.14202/vetworld.2023.601-606Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breedJuan Camilo Taborda-Charris0https://orcid.org/0000-0003-0650-5387Roy Rodríguez-Hernández1https://orcid.org/0000-0002-4187-7381María Paula Herrera-Sánchez2https://orcid.org/0000-0002-1138-1625Heinner Fabian Uribe-García3https://orcid.org/0000-0001-9115-757XRafael J. Otero-Arroyo4https://orcid.org/0000-0002-5692-3010Juan Sebastian Naranjo-Gomez5https://orcid.org/0000-0003-2971-2506Kelly Johanna Lozano-Villegas6https://orcid.org/0000-0003-4525-8403Iang Schroniltgen Rondón-Barragín7https://orcid.org/0000-0001-6980-892XImmunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia.Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia.Grupo de Investigación en Reproducción y Mejoramiento Genético Animal, Facultad de Ciencias Agropecuarias, Universidad de Sucre, Sincelejo 700001, Sucre, Colombia; Laboratorio de Reproducción Animal, Corporación de Ciencias Biotecnológicas, Embriotecno, Montería 230029, Córdoba, Colombia.Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia.Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.Background and Aim: Heat shock proteins are highly conserved proteins that work as molecular chaperones expressed in response to thermal stress. This study aimed to determine the expression profile of genes related to the heat stress response in whole blood obtained from the Romosinuano creole breed. Materials and Methods: Real-time polymerase chain reaction was performed to analyze the transcript of hsp90, hsp70, hsp60, and hsf1 in the whole blood of Romosinuano under different temperature-humidity indices (THIs). Results: The expression levels of the hsp70 and hsf1 genes at the high-THI level were higher (p = 0.0011 and p = 0.0003, respectively) than those at the low-THI level. In addition, no differences in the expression levels of the hsp60 and hsP90 genes were detected between the two THIs. Conclusion: The overexpression of hsf1 and hsp70 genes play an important role in protecting cells from damage induced by heat stress.https://www.veterinaryworld.org/Vol.16/March-2023/22.pdfclimate changeexternal environmentheat shock proteinsheat stressmrnatemperature-humidity index |
| spellingShingle | Juan Camilo Taborda-Charris Roy Rodríguez-Hernández María Paula Herrera-Sánchez Heinner Fabian Uribe-García Rafael J. Otero-Arroyo Juan Sebastian Naranjo-Gomez Kelly Johanna Lozano-Villegas Iang Schroniltgen Rondón-Barragín Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed Veterinary World climate change external environment heat shock proteins heat stress mrna temperature-humidity index |
| title | Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed |
| title_full | Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed |
| title_fullStr | Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed |
| title_full_unstemmed | Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed |
| title_short | Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed |
| title_sort | expression profiling of heat shock protein genes in whole blood of romosinuano cattle breed |
| topic | climate change external environment heat shock proteins heat stress mrna temperature-humidity index |
| url | https://www.veterinaryworld.org/Vol.16/March-2023/22.pdf |
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