Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy
Lipopolysaccharide (LPS), an endotoxin derived from the outer membrane of Gram-negative bacteria, mediates a local or systemic inflammatory response. Its action is carried out through stimulation of Tolllike receptor 4 (TLR4) activity and subsequently, production of interleukin-1β (IL-1β), interleuk...
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| Format: | Article |
| Language: | English |
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South Valley University
2022-12-01
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| Series: | SVU-International Journal of Veterinary Sciences |
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| Online Access: | https://svu.journals.ekb.eg/article_277232.html |
| _version_ | 1797799346190680064 |
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| author | Azza A. Abd Elkareem Abd Elkareem Zeinab Al-Amgad Hassan Ahmed |
| author_facet | Azza A. Abd Elkareem Abd Elkareem Zeinab Al-Amgad Hassan Ahmed |
| author_sort | Azza A. Abd Elkareem Abd Elkareem |
| collection | DOAJ |
| description | Lipopolysaccharide (LPS), an endotoxin derived from the outer membrane of Gram-negative bacteria, mediates a local or systemic inflammatory response. Its action is carried out through stimulation of Tolllike receptor 4 (TLR4) activity and subsequently, production of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-gamma (INF- γ). The purpose of this study was to assess the effect of L-arginine (L-Arg) on endocrine pancreas function in rats treated with LPS. Therefore, rats were weighed, randomly divided into 4 equal groups (20 rats/group) as each group using 4 replications (n=5)and intraperitoneally injected as follow: Control group (saline, 1ml/Kg b.wt for 7 consecutive days), LPS group (LPS, 1 mg/kg b.wt once), L-Arg group (L-Arg, 10 mg/kg b.wt, for 7 days), and LPS+L-Arg group (L-Arg, 10 mg/kg b.wt, for 7 days then once injected with LPS, 1mg/kg). Serum IL-6, glucose and insulin levels were assessed at 6, 12, 24, and 72 hours after LPS or saline injection. Histological examination of the pancreas tissue was also performed. Serum IL-6 elevated significantly 6 and 12 hours after LPS injection and retrieved at 24 and 72 hours. Association of LPS and L-Arg potentiate IL-6 production at 6 and 12 hours higher than that in L-Arg-treated group. Serum glucose levels were declined in single LPS treated rats 6 and 12 hours and recovered at 24 and 72 hours after LPS injection. On contrary, Serum glucose levels were elevated 6 and 12 hours and declined near to control level 24 and 72 h after L-Arg injection. However, insulin levels were slightly elevated 12, 24 and 72 hours after LPS injection and 12 hours after LPS+L-Arg injection the rescued around the normal values at 24 and 72 hours. On contrary, serum insulin level decrease at 6 hours after L-Arg injection then recovered to control level. Histologically, rats treated with LPS showed necrosis, vacuolation in islets of Langerhans with infiltration of inflammatory cells. Pre-treatment of L-Arg prior LPS attenuated the alteration in pancreas tissue architecture. However, Rats treated with L-Arg showed normal pancreas tissue. In conclusion, the results of this study support the hypothesized relationship between L-Arg and modulation of glucose metabolism and suggest that L-Arg may have a dual role in the modulation of glucose homeostasis depending on intensity and stage of inflammation. |
| first_indexed | 2024-03-13T04:17:28Z |
| format | Article |
| id | doaj.art-2eee4914cb30474c9bddb4ea3f6ae01e |
| institution | Directory Open Access Journal |
| issn | 2535-1826 2535-1877 |
| language | English |
| last_indexed | 2024-03-13T04:17:28Z |
| publishDate | 2022-12-01 |
| publisher | South Valley University |
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| series | SVU-International Journal of Veterinary Sciences |
| spelling | doaj.art-2eee4914cb30474c9bddb4ea3f6ae01e2023-06-20T18:23:47ZengSouth Valley UniversitySVU-International Journal of Veterinary Sciences2535-18262535-18772022-12-015413815210.21608/SVU.2022.153308.1218Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-StrategyAzza A. Abd Elkareem Abd Elkareem0Zeinab Al-Amgad1Hassan Ahmed2Physiology department, Faculty of Veterinary Medicine, South Valley University, Qena, EgyptGeneral Authority for Veterinary Services, Ph.D in Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena, EgyptPhysiology department, Faculty of Veterinary Medicine, South Valley University, Qena, EgyptLipopolysaccharide (LPS), an endotoxin derived from the outer membrane of Gram-negative bacteria, mediates a local or systemic inflammatory response. Its action is carried out through stimulation of Tolllike receptor 4 (TLR4) activity and subsequently, production of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-gamma (INF- γ). The purpose of this study was to assess the effect of L-arginine (L-Arg) on endocrine pancreas function in rats treated with LPS. Therefore, rats were weighed, randomly divided into 4 equal groups (20 rats/group) as each group using 4 replications (n=5)and intraperitoneally injected as follow: Control group (saline, 1ml/Kg b.wt for 7 consecutive days), LPS group (LPS, 1 mg/kg b.wt once), L-Arg group (L-Arg, 10 mg/kg b.wt, for 7 days), and LPS+L-Arg group (L-Arg, 10 mg/kg b.wt, for 7 days then once injected with LPS, 1mg/kg). Serum IL-6, glucose and insulin levels were assessed at 6, 12, 24, and 72 hours after LPS or saline injection. Histological examination of the pancreas tissue was also performed. Serum IL-6 elevated significantly 6 and 12 hours after LPS injection and retrieved at 24 and 72 hours. Association of LPS and L-Arg potentiate IL-6 production at 6 and 12 hours higher than that in L-Arg-treated group. Serum glucose levels were declined in single LPS treated rats 6 and 12 hours and recovered at 24 and 72 hours after LPS injection. On contrary, Serum glucose levels were elevated 6 and 12 hours and declined near to control level 24 and 72 h after L-Arg injection. However, insulin levels were slightly elevated 12, 24 and 72 hours after LPS injection and 12 hours after LPS+L-Arg injection the rescued around the normal values at 24 and 72 hours. On contrary, serum insulin level decrease at 6 hours after L-Arg injection then recovered to control level. Histologically, rats treated with LPS showed necrosis, vacuolation in islets of Langerhans with infiltration of inflammatory cells. Pre-treatment of L-Arg prior LPS attenuated the alteration in pancreas tissue architecture. However, Rats treated with L-Arg showed normal pancreas tissue. In conclusion, the results of this study support the hypothesized relationship between L-Arg and modulation of glucose metabolism and suggest that L-Arg may have a dual role in the modulation of glucose homeostasis depending on intensity and stage of inflammation. https://svu.journals.ekb.eg/article_277232.htmlinsulinl-argininelipopolysaccharidesnitric oxidepancreas. |
| spellingShingle | Azza A. Abd Elkareem Abd Elkareem Zeinab Al-Amgad Hassan Ahmed Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy SVU-International Journal of Veterinary Sciences insulin l-arginine lipopolysaccharides nitric oxide pancreas. |
| title | Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy |
| title_full | Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy |
| title_fullStr | Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy |
| title_full_unstemmed | Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy |
| title_short | Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy |
| title_sort | modulation of glucose homeostasis in rats treated with bacterial lipopolysaccharide role of l arginine counter strategy |
| topic | insulin l-arginine lipopolysaccharides nitric oxide pancreas. |
| url | https://svu.journals.ekb.eg/article_277232.html |
| work_keys_str_mv | AT azzaaabdelkareemabdelkareem modulationofglucosehomeostasisinratstreatedwithbacteriallipopolysaccharideroleoflargininecounterstrategy AT zeinabalamgad modulationofglucosehomeostasisinratstreatedwithbacteriallipopolysaccharideroleoflargininecounterstrategy AT hassanahmed modulationofglucosehomeostasisinratstreatedwithbacteriallipopolysaccharideroleoflargininecounterstrategy |