Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers
Psoriasis vulgaris (PsV) and psoriatic arthritis (PsA) are inflammatory diseases with unresolved pathophysiological aspects. Extracellular vesicles (EVs) play an important role in intercellular communication. We compared the miRNA contents and surface proteome of the EVs in the blood serum of PsV an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/7/4005 |
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| author | Freddy Lättekivi Irina Guljavina Getnet Midekessa Janeli Viil Paul R. Heath Rikke Bæk Malene Møller Jørgensen Aneta Andronowska Kulli Kingo Alireza Fazeli |
| author_facet | Freddy Lättekivi Irina Guljavina Getnet Midekessa Janeli Viil Paul R. Heath Rikke Bæk Malene Møller Jørgensen Aneta Andronowska Kulli Kingo Alireza Fazeli |
| author_sort | Freddy Lättekivi |
| collection | DOAJ |
| description | Psoriasis vulgaris (PsV) and psoriatic arthritis (PsA) are inflammatory diseases with unresolved pathophysiological aspects. Extracellular vesicles (EVs) play an important role in intercellular communication. We compared the miRNA contents and surface proteome of the EVs in the blood serum of PsV and PsA patients to healthy controls. Size-exclusion chromatography was used to isolate EVs from the blood serum of 12 PsV patients, 12 PsA patients and 12 healthy control subjects. EV samples were characterized and RNA sequencing was used to identify differentially enriched EV-bound miRNAs. We found 212 differentially enriched EV-bound miRNAs present in both PsV and PsA groups—a total of 13 miRNAs at FDR ≤ 0.05. The predicted target genes of these miRNAs were significantly related to lesser known but potentially disease-relevant pathways. The EV array revealed that PsV patient EV samples were significantly enriched with CD9 EV-marker compared to controls. Analysis of EV-bound miRNAs suggests that signaling via EVs in the blood serum could play a role in the pathophysiological processes of PsV and PsA. EVs may be able to fill the void in clinically applicable diagnostic and prognostic biomarkers for PsV and PsA. |
| first_indexed | 2024-03-09T11:44:17Z |
| format | Article |
| id | doaj.art-2ef5a719fd5b4e1a9898cd16d74728dd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T11:44:17Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2ef5a719fd5b4e1a9898cd16d74728dd2023-11-30T23:25:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01237400510.3390/ijms23074005Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease BiomarkersFreddy Lättekivi0Irina Guljavina1Getnet Midekessa2Janeli Viil3Paul R. Heath4Rikke Bæk5Malene Møller Jørgensen6Aneta Andronowska7Kulli Kingo8Alireza Fazeli9Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila St. 14b, 50411 Tartu, EstoniaDepartment of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila St. 14b, 50411 Tartu, EstoniaDepartment of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila St. 14b, 50411 Tartu, EstoniaDepartment of Pharmacology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila St. 14b, 50411 Tartu, EstoniaSheffield Institute of Translational Neuroscience, University of Sheffield, Sheffield S10 2HQ, UKDepartment of Clinical Immunology, Aalborg University Hospital, Urbansgade 32-36, 9000 Aalborg, DenmarkDepartment of Clinical Immunology, Aalborg University Hospital, Urbansgade 32-36, 9000 Aalborg, DenmarkDepartment of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima St. 10, 10-748 Olsztyn, PolandClinic of Dermatology, Institute of Clinical Medicine, University of Tartu, Raja 31, 50417 Tartu, EstoniaDepartment of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila St. 14b, 50411 Tartu, EstoniaPsoriasis vulgaris (PsV) and psoriatic arthritis (PsA) are inflammatory diseases with unresolved pathophysiological aspects. Extracellular vesicles (EVs) play an important role in intercellular communication. We compared the miRNA contents and surface proteome of the EVs in the blood serum of PsV and PsA patients to healthy controls. Size-exclusion chromatography was used to isolate EVs from the blood serum of 12 PsV patients, 12 PsA patients and 12 healthy control subjects. EV samples were characterized and RNA sequencing was used to identify differentially enriched EV-bound miRNAs. We found 212 differentially enriched EV-bound miRNAs present in both PsV and PsA groups—a total of 13 miRNAs at FDR ≤ 0.05. The predicted target genes of these miRNAs were significantly related to lesser known but potentially disease-relevant pathways. The EV array revealed that PsV patient EV samples were significantly enriched with CD9 EV-marker compared to controls. Analysis of EV-bound miRNAs suggests that signaling via EVs in the blood serum could play a role in the pathophysiological processes of PsV and PsA. EVs may be able to fill the void in clinically applicable diagnostic and prognostic biomarkers for PsV and PsA.https://www.mdpi.com/1422-0067/23/7/4005psoriatic arthritiscirculating EVsmiRNAbiomarkersurface proteome |
| spellingShingle | Freddy Lättekivi Irina Guljavina Getnet Midekessa Janeli Viil Paul R. Heath Rikke Bæk Malene Møller Jørgensen Aneta Andronowska Kulli Kingo Alireza Fazeli Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers International Journal of Molecular Sciences psoriatic arthritis circulating EVs miRNA biomarker surface proteome |
| title | Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers |
| title_full | Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers |
| title_fullStr | Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers |
| title_full_unstemmed | Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers |
| title_short | Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers |
| title_sort | profiling blood serum extracellular vesicles in plaque psoriasis and psoriatic arthritis patients reveals potential disease biomarkers |
| topic | psoriatic arthritis circulating EVs miRNA biomarker surface proteome |
| url | https://www.mdpi.com/1422-0067/23/7/4005 |
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