C2orf62 and TTC17 are involved in actin organization and ciliogenesis in zebrafish and human.
Vertebrate genomes contain around 20,000 protein-encoding genes, of which a large fraction is still not associated with specific functions. A major task in future genomics will thus be to assign physiological roles to all open reading frames revealed by genome sequencing. Here we show that C2orf62,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3903541?pdf=render |
| _version_ | 1818597212225011712 |
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| author | Franck Bontems Richard J Fish Irene Borlat Frédérique Lembo Sophie Chocu Frédéric Chalmel Jean-Paul Borg Charles Pineau Marguerite Neerman-Arbez Amos Bairoch Lydie Lane |
| author_facet | Franck Bontems Richard J Fish Irene Borlat Frédérique Lembo Sophie Chocu Frédéric Chalmel Jean-Paul Borg Charles Pineau Marguerite Neerman-Arbez Amos Bairoch Lydie Lane |
| author_sort | Franck Bontems |
| collection | DOAJ |
| description | Vertebrate genomes contain around 20,000 protein-encoding genes, of which a large fraction is still not associated with specific functions. A major task in future genomics will thus be to assign physiological roles to all open reading frames revealed by genome sequencing. Here we show that C2orf62, a highly conserved protein with little homology to characterized proteins, is strongly expressed in testis in zebrafish and mammals, and in various types of ciliated cells during zebrafish development. By yeast two hybrid and GST pull-down, C2orf62 was shown to interact with TTC17, another uncharacterized protein. Depletion of either C2orf62 or TTC17 in human ciliated cells interferes with actin polymerization and reduces the number of primary cilia without changing their length. Zebrafish embryos injected with morpholinos against C2orf62 or TTC17, or with mRNA coding for the C2orf62 C-terminal part containing a RII dimerization/docking (R2D2) - like domain show morphological defects consistent with imperfect ciliogenesis. We provide here the first evidence for a C2orf62-TTC17 axis that would regulate actin polymerization and ciliogenesis. |
| first_indexed | 2024-12-16T11:44:13Z |
| format | Article |
| id | doaj.art-2ef7a2f973824fbc83d9ea0da4b68fd4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T11:44:13Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2ef7a2f973824fbc83d9ea0da4b68fd42022-12-21T22:32:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8647610.1371/journal.pone.0086476C2orf62 and TTC17 are involved in actin organization and ciliogenesis in zebrafish and human.Franck BontemsRichard J FishIrene BorlatFrédérique LemboSophie ChocuFrédéric ChalmelJean-Paul BorgCharles PineauMarguerite Neerman-ArbezAmos BairochLydie LaneVertebrate genomes contain around 20,000 protein-encoding genes, of which a large fraction is still not associated with specific functions. A major task in future genomics will thus be to assign physiological roles to all open reading frames revealed by genome sequencing. Here we show that C2orf62, a highly conserved protein with little homology to characterized proteins, is strongly expressed in testis in zebrafish and mammals, and in various types of ciliated cells during zebrafish development. By yeast two hybrid and GST pull-down, C2orf62 was shown to interact with TTC17, another uncharacterized protein. Depletion of either C2orf62 or TTC17 in human ciliated cells interferes with actin polymerization and reduces the number of primary cilia without changing their length. Zebrafish embryos injected with morpholinos against C2orf62 or TTC17, or with mRNA coding for the C2orf62 C-terminal part containing a RII dimerization/docking (R2D2) - like domain show morphological defects consistent with imperfect ciliogenesis. We provide here the first evidence for a C2orf62-TTC17 axis that would regulate actin polymerization and ciliogenesis.http://europepmc.org/articles/PMC3903541?pdf=render |
| spellingShingle | Franck Bontems Richard J Fish Irene Borlat Frédérique Lembo Sophie Chocu Frédéric Chalmel Jean-Paul Borg Charles Pineau Marguerite Neerman-Arbez Amos Bairoch Lydie Lane C2orf62 and TTC17 are involved in actin organization and ciliogenesis in zebrafish and human. PLoS ONE |
| title | C2orf62 and TTC17 are involved in actin organization and ciliogenesis in zebrafish and human. |
| title_full | C2orf62 and TTC17 are involved in actin organization and ciliogenesis in zebrafish and human. |
| title_fullStr | C2orf62 and TTC17 are involved in actin organization and ciliogenesis in zebrafish and human. |
| title_full_unstemmed | C2orf62 and TTC17 are involved in actin organization and ciliogenesis in zebrafish and human. |
| title_short | C2orf62 and TTC17 are involved in actin organization and ciliogenesis in zebrafish and human. |
| title_sort | c2orf62 and ttc17 are involved in actin organization and ciliogenesis in zebrafish and human |
| url | http://europepmc.org/articles/PMC3903541?pdf=render |
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