Conservation of the mycelia of the medicinal mushroom Humphreya coffeata (Berk.) Stey. in sterile distilled water
Currently, there is a growing interest in obtaining and studying the biologically active compounds from higher basidiomycetes, such as Ganoderma lucidum, Lentinus edodes and Inonotus obliquus [1], but the techniques for safe long-term storage are time-consuming, susceptible to contamination, and do...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016114200185 |
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| author | Monserrat García-García Leticia Rocha-Zavaleta Norma A. Valdez-Cruz Mauricio A. Trujillo-Roldán |
| author_facet | Monserrat García-García Leticia Rocha-Zavaleta Norma A. Valdez-Cruz Mauricio A. Trujillo-Roldán |
| author_sort | Monserrat García-García |
| collection | DOAJ |
| description | Currently, there is a growing interest in obtaining and studying the biologically active compounds from higher basidiomycetes, such as Ganoderma lucidum, Lentinus edodes and Inonotus obliquus [1], but the techniques for safe long-term storage are time-consuming, susceptible to contamination, and do not prevent genetic and physiological changes during long-term maintenance [2]. A recent strategy for obtaining biologically active compounds is using mycelia submerged cultures of these mushrooms, cultured under controlled laboratory conditions [1]. However, obtaining spores of these fungi under these conditions is difficult, and in most cases the way to obtain the spores is unknown [1]. Therefore, the strategy for mycelium storage seems to be more appropriated and simple.•
A modification of Castellani's method [3–7] is proposed for higher basidiomycetes, by using the mycelium of Humphreya coffeata (Berk.) Stey., whose culture filtrates demonstrated bioactivity against lymphoma cells [8].
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H. coffeata (Berk.) Stey. was grown on malt extract agar with filter paper disks that were removed after 4 days, placed in tubes with sterile distilled water, and stored at 4 °C.
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Filter paper disks with H. coffeata (Berk.) Stey. stored at 4 °C were confirmed to be viable for up to 18 months, with no visible morphological alterations. |
| first_indexed | 2024-12-11T02:48:19Z |
| format | Article |
| id | doaj.art-2efa6d9f74fa497a99267ed5775d3e24 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-12-11T02:48:19Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-2efa6d9f74fa497a99267ed5775d3e242022-12-22T01:23:22ZengElsevierMethodsX2215-01612014-01-011C192210.1016/j.mex.2014.04.002Conservation of the mycelia of the medicinal mushroom Humphreya coffeata (Berk.) Stey. in sterile distilled waterMonserrat García-GarcíaLeticia Rocha-ZavaletaNorma A. Valdez-CruzMauricio A. Trujillo-RoldánCurrently, there is a growing interest in obtaining and studying the biologically active compounds from higher basidiomycetes, such as Ganoderma lucidum, Lentinus edodes and Inonotus obliquus [1], but the techniques for safe long-term storage are time-consuming, susceptible to contamination, and do not prevent genetic and physiological changes during long-term maintenance [2]. A recent strategy for obtaining biologically active compounds is using mycelia submerged cultures of these mushrooms, cultured under controlled laboratory conditions [1]. However, obtaining spores of these fungi under these conditions is difficult, and in most cases the way to obtain the spores is unknown [1]. Therefore, the strategy for mycelium storage seems to be more appropriated and simple.• A modification of Castellani's method [3–7] is proposed for higher basidiomycetes, by using the mycelium of Humphreya coffeata (Berk.) Stey., whose culture filtrates demonstrated bioactivity against lymphoma cells [8]. • H. coffeata (Berk.) Stey. was grown on malt extract agar with filter paper disks that were removed after 4 days, placed in tubes with sterile distilled water, and stored at 4 °C. • Filter paper disks with H. coffeata (Berk.) Stey. stored at 4 °C were confirmed to be viable for up to 18 months, with no visible morphological alterations.http://www.sciencedirect.com/science/article/pii/S2215016114200185Preservation of mycelial cultures of basidiomycetes |
| spellingShingle | Monserrat García-García Leticia Rocha-Zavaleta Norma A. Valdez-Cruz Mauricio A. Trujillo-Roldán Conservation of the mycelia of the medicinal mushroom Humphreya coffeata (Berk.) Stey. in sterile distilled water MethodsX Preservation of mycelial cultures of basidiomycetes |
| title | Conservation of the mycelia of the medicinal mushroom Humphreya coffeata (Berk.) Stey. in sterile distilled water |
| title_full | Conservation of the mycelia of the medicinal mushroom Humphreya coffeata (Berk.) Stey. in sterile distilled water |
| title_fullStr | Conservation of the mycelia of the medicinal mushroom Humphreya coffeata (Berk.) Stey. in sterile distilled water |
| title_full_unstemmed | Conservation of the mycelia of the medicinal mushroom Humphreya coffeata (Berk.) Stey. in sterile distilled water |
| title_short | Conservation of the mycelia of the medicinal mushroom Humphreya coffeata (Berk.) Stey. in sterile distilled water |
| title_sort | conservation of the mycelia of the medicinal mushroom humphreya coffeata berk stey in sterile distilled water |
| topic | Preservation of mycelial cultures of basidiomycetes |
| url | http://www.sciencedirect.com/science/article/pii/S2215016114200185 |
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