Epstein-Barr virus infection and breast invasive ductal carcinoma in Egyptian women: A single center experience
Background: A controversy of the role of Epstein-Barr virus (EBV) infection in breast carcinomas has been reported in the literature. Objectives: We carried on this research to explore possible association between EBV infection and breast invasive ductal carcinoma (IDC) in Egyptian women attending o...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2017-06-01
|
| Series: | Journal of the Egyptian National Cancer Institute |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110036217300080 |
| _version_ | 1818507144533639168 |
|---|---|
| author | Noha ED Hassab El-Naby Hameda Hassan Mohamed Asmaa Mohamed Goda Ahmed El Sayed Mohamed |
| author_facet | Noha ED Hassab El-Naby Hameda Hassan Mohamed Asmaa Mohamed Goda Ahmed El Sayed Mohamed |
| author_sort | Noha ED Hassab El-Naby |
| collection | DOAJ |
| description | Background: A controversy of the role of Epstein-Barr virus (EBV) infection in breast carcinomas has been reported in the literature.
Objectives: We carried on this research to explore possible association between EBV infection and breast invasive ductal carcinoma (IDC) in Egyptian women attending our center.
Study design: This study carried out at Sohag university hospital on 84 paraffin embedded samples of breast tissue, of them 42 breast IDC as the case group and 42 breast fibroadenomas as the control group. Nested PCR and immunohistochemistry (IHC) done separately for all samples to identify the Epstein-Barr nuclear antigen-1 (EBNA-1) gene and EBV latent membrane protein-1 (LMP-1) respectively, in breast cancer cells and controls.
Results: Specimen considered positive when both (EBNA-1) gene and LMP-1 were detected using PCR and IHC separately for the same sample, this was achieved by 10/42 (23.81%) of breast IDC (case group) and 6/42 (14.29%) of breast fibro-adenomas (control group) (P-value = 0.4). Nodal involvement was the only parameter that demonstrated a significant statistical relationship with EBV presence in cancerous tissue with p-value = 0.003.
Conclusion: Our research could not find a significant statistical association between EBV infection and breast IDC in Egyptian women attending our center, but, there might be an association between the existence of EBV and tumor aggressiveness. |
| first_indexed | 2024-12-10T22:14:33Z |
| format | Article |
| id | doaj.art-2eff40d21aa14fef97d7b8930f687a4f |
| institution | Directory Open Access Journal |
| issn | 1110-0362 |
| language | English |
| last_indexed | 2024-12-10T22:14:33Z |
| publishDate | 2017-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of the Egyptian National Cancer Institute |
| spelling | doaj.art-2eff40d21aa14fef97d7b8930f687a4f2022-12-22T01:31:31ZengSpringerOpenJournal of the Egyptian National Cancer Institute1110-03622017-06-01292778210.1016/j.jnci.2017.02.002Epstein-Barr virus infection and breast invasive ductal carcinoma in Egyptian women: A single center experienceNoha ED Hassab El-Naby0Hameda Hassan Mohamed1Asmaa Mohamed Goda2Ahmed El Sayed Mohamed3Department of Pathology, Sohag University, Faculty of Medicine, EgyptDepartment of Medical Microbiology and Immunology, Sohag Faculty of Medicine, Sohag University, EgyptDepartment of Medical Microbiology and Immunology, Sohag Faculty of Medicine, Sohag University, EgyptDepartment of Clinical and Radiation Oncology, Sohag University, Faculty of Medicine, EgyptBackground: A controversy of the role of Epstein-Barr virus (EBV) infection in breast carcinomas has been reported in the literature. Objectives: We carried on this research to explore possible association between EBV infection and breast invasive ductal carcinoma (IDC) in Egyptian women attending our center. Study design: This study carried out at Sohag university hospital on 84 paraffin embedded samples of breast tissue, of them 42 breast IDC as the case group and 42 breast fibroadenomas as the control group. Nested PCR and immunohistochemistry (IHC) done separately for all samples to identify the Epstein-Barr nuclear antigen-1 (EBNA-1) gene and EBV latent membrane protein-1 (LMP-1) respectively, in breast cancer cells and controls. Results: Specimen considered positive when both (EBNA-1) gene and LMP-1 were detected using PCR and IHC separately for the same sample, this was achieved by 10/42 (23.81%) of breast IDC (case group) and 6/42 (14.29%) of breast fibro-adenomas (control group) (P-value = 0.4). Nodal involvement was the only parameter that demonstrated a significant statistical relationship with EBV presence in cancerous tissue with p-value = 0.003. Conclusion: Our research could not find a significant statistical association between EBV infection and breast IDC in Egyptian women attending our center, but, there might be an association between the existence of EBV and tumor aggressiveness.http://www.sciencedirect.com/science/article/pii/S1110036217300080Epstein-Barr virusBreast invasiveDuctal carcinomaNested PCRImmunohistochemistry |
| spellingShingle | Noha ED Hassab El-Naby Hameda Hassan Mohamed Asmaa Mohamed Goda Ahmed El Sayed Mohamed Epstein-Barr virus infection and breast invasive ductal carcinoma in Egyptian women: A single center experience Journal of the Egyptian National Cancer Institute Epstein-Barr virus Breast invasive Ductal carcinoma Nested PCR Immunohistochemistry |
| title | Epstein-Barr virus infection and breast invasive ductal carcinoma in Egyptian women: A single center experience |
| title_full | Epstein-Barr virus infection and breast invasive ductal carcinoma in Egyptian women: A single center experience |
| title_fullStr | Epstein-Barr virus infection and breast invasive ductal carcinoma in Egyptian women: A single center experience |
| title_full_unstemmed | Epstein-Barr virus infection and breast invasive ductal carcinoma in Egyptian women: A single center experience |
| title_short | Epstein-Barr virus infection and breast invasive ductal carcinoma in Egyptian women: A single center experience |
| title_sort | epstein barr virus infection and breast invasive ductal carcinoma in egyptian women a single center experience |
| topic | Epstein-Barr virus Breast invasive Ductal carcinoma Nested PCR Immunohistochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S1110036217300080 |
| work_keys_str_mv | AT nohaedhassabelnaby epsteinbarrvirusinfectionandbreastinvasiveductalcarcinomainegyptianwomenasinglecenterexperience AT hamedahassanmohamed epsteinbarrvirusinfectionandbreastinvasiveductalcarcinomainegyptianwomenasinglecenterexperience AT asmaamohamedgoda epsteinbarrvirusinfectionandbreastinvasiveductalcarcinomainegyptianwomenasinglecenterexperience AT ahmedelsayedmohamed epsteinbarrvirusinfectionandbreastinvasiveductalcarcinomainegyptianwomenasinglecenterexperience |