Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum)
A new method of transgenic development called “In-planta” transformation method, where Agrobacterium is used to infect the plantlets but the steps of in vitro regeneration of plants is totally avoided. In this study, we have reported a simple In-planta method for efficient transformation of diploid...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-06-01
|
| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X16300105 |
| _version_ | 1797204789201731584 |
|---|---|
| author | Bipinchandra B. Kalbande Anita S. Patil |
| author_facet | Bipinchandra B. Kalbande Anita S. Patil |
| author_sort | Bipinchandra B. Kalbande |
| collection | DOAJ |
| description | A new method of transgenic development called “In-planta” transformation method, where Agrobacterium is used to infect the plantlets but the steps of in vitro regeneration of plants is totally avoided. In this study, we have reported a simple In-planta method for efficient transformation of diploid cotton Gossypium hirsutum cv LRK-516 Anjali using Agrobacterium tumefaciens EHA-105 harbouring recombinant binary vector plasmid pBinAR with Arabidopsis At-NPR1 gene. Four day old plantlets were used for transformation. A vertical cut was made at the junction of cotyledonary leaves, moderately bisecting the shoot tip and exposing meristem cells at apical meristem. This site was infected with Agrobacterium inoculum. The transgenic events obtained were tested positive for the presence of At-NPR1 gene with promoter nptII gene. They are also tested negative for vector backbone integration and Agrobacterium contamination in T0 events. With this method a transformation frequency of 6.89% was reported for the cv LRK-516. |
| first_indexed | 2024-04-24T08:40:48Z |
| format | Article |
| id | doaj.art-2eff9007f3ae467bb7f95c432e8c5de8 |
| institution | Directory Open Access Journal |
| issn | 1687-157X |
| language | English |
| last_indexed | 2024-04-24T08:40:48Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-2eff9007f3ae467bb7f95c432e8c5de82024-04-16T16:03:37ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2016-06-0114191810.1016/j.jgeb.2016.05.003Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum)Bipinchandra B. KalbandeAnita S. PatilA new method of transgenic development called “In-planta” transformation method, where Agrobacterium is used to infect the plantlets but the steps of in vitro regeneration of plants is totally avoided. In this study, we have reported a simple In-planta method for efficient transformation of diploid cotton Gossypium hirsutum cv LRK-516 Anjali using Agrobacterium tumefaciens EHA-105 harbouring recombinant binary vector plasmid pBinAR with Arabidopsis At-NPR1 gene. Four day old plantlets were used for transformation. A vertical cut was made at the junction of cotyledonary leaves, moderately bisecting the shoot tip and exposing meristem cells at apical meristem. This site was infected with Agrobacterium inoculum. The transgenic events obtained were tested positive for the presence of At-NPR1 gene with promoter nptII gene. They are also tested negative for vector backbone integration and Agrobacterium contamination in T0 events. With this method a transformation frequency of 6.89% was reported for the cv LRK-516.http://www.sciencedirect.com/science/article/pii/S1687157X16300105In-planta transformationDiploid cottonGossypium hirsutum cv LRK-516 AnjalipBinARAt-NPR1 geneCotyledonary leavesMeristem cellsTransformation frequency |
| spellingShingle | Bipinchandra B. Kalbande Anita S. Patil Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum) Journal of Genetic Engineering and Biotechnology In-planta transformation Diploid cotton Gossypium hirsutum cv LRK-516 Anjali pBinAR At-NPR1 gene Cotyledonary leaves Meristem cells Transformation frequency |
| title | Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum) |
| title_full | Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum) |
| title_fullStr | Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum) |
| title_full_unstemmed | Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum) |
| title_short | Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum) |
| title_sort | plant tissue culture independent agrobacterium tumefaciens mediated in planta transformation strategy for upland cotton gossypium hirsutum |
| topic | In-planta transformation Diploid cotton Gossypium hirsutum cv LRK-516 Anjali pBinAR At-NPR1 gene Cotyledonary leaves Meristem cells Transformation frequency |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X16300105 |
| work_keys_str_mv | AT bipinchandrabkalbande planttissuecultureindependentagrobacteriumtumefaciensmediatedinplantatransformationstrategyforuplandcottongossypiumhirsutum AT anitaspatil planttissuecultureindependentagrobacteriumtumefaciensmediatedinplantatransformationstrategyforuplandcottongossypiumhirsutum |