Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa
Pseudomonas aeruginosa is considered one of the most important hospital germs; its isolation is common in hospitalized patients. In addition, this microorganism has a marked multiresistance, which increases mortality. Treatment of these patients is often difficult, since in addition to its natural r...
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| Format: | Article |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2012-07-01
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| Series: | Kasmera |
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| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/4936 |
| _version_ | 1827853548542492672 |
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| author | Armindo José Perozo Mena Maribel Josefina Castellano González Eliana Ling Toledo Nailet Arraiz |
| author_facet | Armindo José Perozo Mena Maribel Josefina Castellano González Eliana Ling Toledo Nailet Arraiz |
| author_sort | Armindo José Perozo Mena |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is considered one of the most important hospital germs; its isolation is common in hospitalized patients. In addition, this microorganism has a marked multiresistance, which increases mortality. Treatment of these patients is often difficult, since in addition to its natural resistance, Pseudomonas can obtain resistance mechanisms to virtually all antimicrobial drugs available for its treatment; due to this, its appearance is increasingly frequent and necessitates the use of antibiotics such as carbapenems, which facilitates the acquisition of resistance mechanisms to these drugs. This study attempts to determine the production of metallobeta-B-lactamase (MBL) in clinical isolates of Pseudomonas aeruginosa, utilizing two phenotypic methods: the double disc method (MDD) and the modified Hodge test (MHT). 726 clinical isolates of P. aeruginosa were analyzed; 20.11% (146) of these were resistant to imipenem (IPM) and meropenem (MEM); 139 were positive for the MDD, while 144 were positive for the MHT. These two methods permitted confirming the presence of an MBL-type carbapenemase in 98.63% of P. aeruginosa isolates; five isolates were negative for the MDD but positive for the MHT, indicating the presence of non-MBL-type carbapenemase in these isolates. Also, 2 isolates were obtained that, despite being resistant to IPM and MEM, were negative according to the two phenotypic methods used; this would indicate the presence of a non-enzymatic resistance mechanism conferring resistance to carbapenems. The use of phenotypic methods for detecting MBL in P. aeruginosa isolates is quite an acceptable option for use in routine laboratories where specialized molecular biology tests are not available. |
| first_indexed | 2024-03-12T11:08:21Z |
| format | Article |
| id | doaj.art-2effb701c5494cbab70535aaf0898604 |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T11:08:21Z |
| publishDate | 2012-07-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-2effb701c5494cbab70535aaf08986042023-09-02T03:25:30ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282012-07-014021131214935Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosaArmindo José Perozo Mena0Maribel Josefina Castellano González1Eliana Ling Toledo2Nailet Arraiz3Escuela de Bioanálisis, Facultad de Medicina, Universidad del Zulia. Centro de Referencia Bacteriológica, Servicio Autónomo Hospital Universitario de Maracaibo.Escuela de Bioanálisis, Facultad de Medicina, Universidad del Zulia.Centro de Referencia Bacteriológica, Servicio Autónomo Hospital Universitario de Maracaibo.Escuela de Bioanálisis, Facultad de Medicina, Universidad del Zulia. Laboratorio de Biología Molecular, Centro de Investigaciones Endocrino Metabólicas Dr. Feliz Gómez, Facultad de Medicina, Universidad del ZuliaPseudomonas aeruginosa is considered one of the most important hospital germs; its isolation is common in hospitalized patients. In addition, this microorganism has a marked multiresistance, which increases mortality. Treatment of these patients is often difficult, since in addition to its natural resistance, Pseudomonas can obtain resistance mechanisms to virtually all antimicrobial drugs available for its treatment; due to this, its appearance is increasingly frequent and necessitates the use of antibiotics such as carbapenems, which facilitates the acquisition of resistance mechanisms to these drugs. This study attempts to determine the production of metallobeta-B-lactamase (MBL) in clinical isolates of Pseudomonas aeruginosa, utilizing two phenotypic methods: the double disc method (MDD) and the modified Hodge test (MHT). 726 clinical isolates of P. aeruginosa were analyzed; 20.11% (146) of these were resistant to imipenem (IPM) and meropenem (MEM); 139 were positive for the MDD, while 144 were positive for the MHT. These two methods permitted confirming the presence of an MBL-type carbapenemase in 98.63% of P. aeruginosa isolates; five isolates were negative for the MDD but positive for the MHT, indicating the presence of non-MBL-type carbapenemase in these isolates. Also, 2 isolates were obtained that, despite being resistant to IPM and MEM, were negative according to the two phenotypic methods used; this would indicate the presence of a non-enzymatic resistance mechanism conferring resistance to carbapenems. The use of phenotypic methods for detecting MBL in P. aeruginosa isolates is quite an acceptable option for use in routine laboratories where specialized molecular biology tests are not available.https://produccioncientificaluz.org/index.php/kasmera/article/view/4936pseudomonas aeruginosaMBLimipenemmeropenem |
| spellingShingle | Armindo José Perozo Mena Maribel Josefina Castellano González Eliana Ling Toledo Nailet Arraiz Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa Kasmera pseudomonas aeruginosa MBL imipenem meropenem |
| title | Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
| title_full | Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
| title_fullStr | Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
| title_full_unstemmed | Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
| title_short | Phenotypic Detection of Metallo-beta-lactamase in Clinical Isolates of Pseudomonas aeruginosa |
| title_sort | phenotypic detection of metallo beta lactamase in clinical isolates of pseudomonas aeruginosa |
| topic | pseudomonas aeruginosa MBL imipenem meropenem |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/4936 |
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