A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion
Abstract Membrane fusion is an essential step for the entry of enveloped viruses, such as human immunodeficiency virus and influenza virus, into the host cell, often triggered by the binding of membrane proteins on the viral envelope to host cell membrane. Recently, external stimuli was shown to tri...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47347-7 |
| _version_ | 1797576823029104640 |
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| author | Hiroto Furukawa Yuuna Kimura Hiroshi Inaba Kazunori Matsuura |
| author_facet | Hiroto Furukawa Yuuna Kimura Hiroshi Inaba Kazunori Matsuura |
| author_sort | Hiroto Furukawa |
| collection | DOAJ |
| description | Abstract Membrane fusion is an essential step for the entry of enveloped viruses, such as human immunodeficiency virus and influenza virus, into the host cell, often triggered by the binding of membrane proteins on the viral envelope to host cell membrane. Recently, external stimuli was shown to trigger membrane fusion in an artificial system. Direct observation of artificial membrane fusion using a giant unilamellar vesicle (GUV), which is similar in size to a cell, is useful as a biological model system. However, there are no model systems for studying membrane fusion of enveloped viruses with host cells. Here, we report a supramolecular model system for viral entry into a GUV or cell through membrane fusion. The system was constructed by complexing a cationic lipid bilayer on an anionic artificial viral capsid, self-assembled from viral β-annulus peptides. We demonstrate that the cationic enveloped artificial viral capsid electrostatically interacts with the anionic GUV or cell, and the capsid enters the GUV or cell through membrane fusion. The model system established in this study will be important for analyzing membrane fusion during infection of a natural virus. |
| first_indexed | 2024-03-10T21:59:09Z |
| format | Article |
| id | doaj.art-2effb8610a7f4273a5502fa70b09d204 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:59:09Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2effb8610a7f4273a5502fa70b09d2042023-11-19T13:00:09ZengNature PortfolioScientific Reports2045-23222023-11-0113111210.1038/s41598-023-47347-7A supramolecular system mimicking the infection process of an enveloped virus through membrane fusionHiroto Furukawa0Yuuna Kimura1Hiroshi Inaba2Kazunori Matsuura3Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori UniversityDepartment of Chemistry and Biotechnology, Graduate School of Engineering, Tottori UniversityDepartment of Chemistry and Biotechnology, Graduate School of Engineering, Tottori UniversityDepartment of Chemistry and Biotechnology, Graduate School of Engineering, Tottori UniversityAbstract Membrane fusion is an essential step for the entry of enveloped viruses, such as human immunodeficiency virus and influenza virus, into the host cell, often triggered by the binding of membrane proteins on the viral envelope to host cell membrane. Recently, external stimuli was shown to trigger membrane fusion in an artificial system. Direct observation of artificial membrane fusion using a giant unilamellar vesicle (GUV), which is similar in size to a cell, is useful as a biological model system. However, there are no model systems for studying membrane fusion of enveloped viruses with host cells. Here, we report a supramolecular model system for viral entry into a GUV or cell through membrane fusion. The system was constructed by complexing a cationic lipid bilayer on an anionic artificial viral capsid, self-assembled from viral β-annulus peptides. We demonstrate that the cationic enveloped artificial viral capsid electrostatically interacts with the anionic GUV or cell, and the capsid enters the GUV or cell through membrane fusion. The model system established in this study will be important for analyzing membrane fusion during infection of a natural virus.https://doi.org/10.1038/s41598-023-47347-7 |
| spellingShingle | Hiroto Furukawa Yuuna Kimura Hiroshi Inaba Kazunori Matsuura A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion Scientific Reports |
| title | A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion |
| title_full | A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion |
| title_fullStr | A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion |
| title_full_unstemmed | A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion |
| title_short | A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion |
| title_sort | supramolecular system mimicking the infection process of an enveloped virus through membrane fusion |
| url | https://doi.org/10.1038/s41598-023-47347-7 |
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