Piperine pretreatment attenuates renal ischemia-reperfusion induced liver injury
Background: Remote organ injury is one of the complications which are developed following ischemia-reperfusion induced acute kidney injury (AKI), dramatically increasing its mortality rate. The aim of the present study was to investigate the effect of piperine pretreatment on liver dysfunction follo...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019358402 |
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| author | Maryam Mohammadi Houshang Najafi Zeynab Mohamadi Yarijani Gholamhasan Vaezi Vida Hojati |
| author_facet | Maryam Mohammadi Houshang Najafi Zeynab Mohamadi Yarijani Gholamhasan Vaezi Vida Hojati |
| author_sort | Maryam Mohammadi |
| collection | DOAJ |
| description | Background: Remote organ injury is one of the complications which are developed following ischemia-reperfusion induced acute kidney injury (AKI), dramatically increasing its mortality rate. The aim of the present study was to investigate the effect of piperine pretreatment on liver dysfunction following ischemia-reperfusion induced AKI. Materials and methods: Acute kidney injury was induced by 30 min-bilateral renal ischemia followed by 24 h of reperfusion. To investigate liver damages, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) enzymes were measured in plasma. In order to study oxidative stress, malondialdehyde (MDA) and ferric reducing antioxidant power (FRAP) levels were measured. Furthermore, the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA along with infiltration of leukocytes in the liver tissue was measured for inflammation assessment. Histopathological damages were studied through measuring the extent of cellular fibrosis, sinusoidal dilatation, and vascular congestion in liver tissue. Results: Following acute kidney injury, AST, ALT, and ALP levels in plasma, MDA level and ICAM-1 expression in the liver tissue, infiltration of leukocytes into the interstitium, and hepatic histopathologic damages increased significantly, while FRAP decreased. Pretreatment with piperine at 10 and 20 mg/kg body weight was able to improve these damages, such that some of them reached its value in the sham group, though piperine in the 20 mg/kg was more effective. Conclusions: The results of this study suggest that ischemia-reperfusion induced AKI result in hepatic damages, and pretreatment with piperine can prevent development of these damages through its antioxidant and anti-inflammatory properties. |
| first_indexed | 2024-12-21T02:12:45Z |
| format | Article |
| id | doaj.art-2f00b4aba06f419ebb35727f83396347 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-21T02:12:45Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-2f00b4aba06f419ebb35727f833963472022-12-21T19:19:19ZengElsevierHeliyon2405-84402019-08-0158e02180Piperine pretreatment attenuates renal ischemia-reperfusion induced liver injuryMaryam Mohammadi0Houshang Najafi1Zeynab Mohamadi Yarijani2Gholamhasan Vaezi3Vida Hojati4Department of Biology, Damghan Branch, Islamic Azad University, Damghan, IranMedical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran; Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran; Corresponding author.Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran; Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Biology, Damghan Branch, Islamic Azad University, Damghan, IranDepartment of Biology, Damghan Branch, Islamic Azad University, Damghan, IranBackground: Remote organ injury is one of the complications which are developed following ischemia-reperfusion induced acute kidney injury (AKI), dramatically increasing its mortality rate. The aim of the present study was to investigate the effect of piperine pretreatment on liver dysfunction following ischemia-reperfusion induced AKI. Materials and methods: Acute kidney injury was induced by 30 min-bilateral renal ischemia followed by 24 h of reperfusion. To investigate liver damages, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) enzymes were measured in plasma. In order to study oxidative stress, malondialdehyde (MDA) and ferric reducing antioxidant power (FRAP) levels were measured. Furthermore, the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA along with infiltration of leukocytes in the liver tissue was measured for inflammation assessment. Histopathological damages were studied through measuring the extent of cellular fibrosis, sinusoidal dilatation, and vascular congestion in liver tissue. Results: Following acute kidney injury, AST, ALT, and ALP levels in plasma, MDA level and ICAM-1 expression in the liver tissue, infiltration of leukocytes into the interstitium, and hepatic histopathologic damages increased significantly, while FRAP decreased. Pretreatment with piperine at 10 and 20 mg/kg body weight was able to improve these damages, such that some of them reached its value in the sham group, though piperine in the 20 mg/kg was more effective. Conclusions: The results of this study suggest that ischemia-reperfusion induced AKI result in hepatic damages, and pretreatment with piperine can prevent development of these damages through its antioxidant and anti-inflammatory properties.http://www.sciencedirect.com/science/article/pii/S2405844019358402BiochemistryPlant biologyPhysiology |
| spellingShingle | Maryam Mohammadi Houshang Najafi Zeynab Mohamadi Yarijani Gholamhasan Vaezi Vida Hojati Piperine pretreatment attenuates renal ischemia-reperfusion induced liver injury Heliyon Biochemistry Plant biology Physiology |
| title | Piperine pretreatment attenuates renal ischemia-reperfusion induced liver injury |
| title_full | Piperine pretreatment attenuates renal ischemia-reperfusion induced liver injury |
| title_fullStr | Piperine pretreatment attenuates renal ischemia-reperfusion induced liver injury |
| title_full_unstemmed | Piperine pretreatment attenuates renal ischemia-reperfusion induced liver injury |
| title_short | Piperine pretreatment attenuates renal ischemia-reperfusion induced liver injury |
| title_sort | piperine pretreatment attenuates renal ischemia reperfusion induced liver injury |
| topic | Biochemistry Plant biology Physiology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019358402 |
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