Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant
Methanotrophs have been used to convert methane to methanol at ambient temperature and pressure. In order to accumulate methanol using methanotrophs, methanol dehydrogenase (MDH) must be downregulated as it consumes methanol. Here, we describe a methanol production system wherein MDH expression is c...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.639266/full |
| _version_ | 1818416236715835392 |
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| author | Hidehiro Ito Hidehiro Ito Kosei Yoshimori Masahito Ishikawa Katsutoshi Hori Toshiaki Kamachi |
| author_facet | Hidehiro Ito Hidehiro Ito Kosei Yoshimori Masahito Ishikawa Katsutoshi Hori Toshiaki Kamachi |
| author_sort | Hidehiro Ito |
| collection | DOAJ |
| description | Methanotrophs have been used to convert methane to methanol at ambient temperature and pressure. In order to accumulate methanol using methanotrophs, methanol dehydrogenase (MDH) must be downregulated as it consumes methanol. Here, we describe a methanol production system wherein MDH expression is controlled by using methanotroph mutants. We used the MxaF knockout mutant of Methylosinus trichosporium OB3b. It could only grow with MDH (XoxF) which has a cerium ion in its active site and is only expressed by bacteria in media containing cerium ions. In the presence of 0 μM copper ion and 25 μM cerium ion, the mutant grew normally. Under conditions conducive to methanol production (10 μM copper ion and 0 μM cerium ion), cell growth was inhibited and methanol accumulated (2.6 μmol·mg−1 dry cell weight·h−1). The conversion efficiency of the accumulated methanol to the total amount of methane added to the reaction system was ~0.3%. The aforementioned conditions were repeatedly alternated by modulating the metal ion composition of the bacterial growth medium. |
| first_indexed | 2024-12-14T11:47:41Z |
| format | Article |
| id | doaj.art-2f04fab712fb49dba90643e68fb58141 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T11:47:41Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2f04fab712fb49dba90643e68fb581412022-12-21T23:02:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-03-011210.3389/fmicb.2021.639266639266Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b MutantHidehiro Ito0Hidehiro Ito1Kosei Yoshimori2Masahito Ishikawa3Katsutoshi Hori4Toshiaki Kamachi5Department of Life Science and Technology, Tokyo Institute of Technology, Tokyo, JapanDepartment of Biomolecular Engineering, Nagoya University, Nagoya, JapanDepartment of Life Science and Technology, Tokyo Institute of Technology, Tokyo, JapanDepartment of Biomolecular Engineering, Nagoya University, Nagoya, JapanDepartment of Biomolecular Engineering, Nagoya University, Nagoya, JapanDepartment of Life Science and Technology, Tokyo Institute of Technology, Tokyo, JapanMethanotrophs have been used to convert methane to methanol at ambient temperature and pressure. In order to accumulate methanol using methanotrophs, methanol dehydrogenase (MDH) must be downregulated as it consumes methanol. Here, we describe a methanol production system wherein MDH expression is controlled by using methanotroph mutants. We used the MxaF knockout mutant of Methylosinus trichosporium OB3b. It could only grow with MDH (XoxF) which has a cerium ion in its active site and is only expressed by bacteria in media containing cerium ions. In the presence of 0 μM copper ion and 25 μM cerium ion, the mutant grew normally. Under conditions conducive to methanol production (10 μM copper ion and 0 μM cerium ion), cell growth was inhibited and methanol accumulated (2.6 μmol·mg−1 dry cell weight·h−1). The conversion efficiency of the accumulated methanol to the total amount of methane added to the reaction system was ~0.3%. The aforementioned conditions were repeatedly alternated by modulating the metal ion composition of the bacterial growth medium.https://www.frontiersin.org/articles/10.3389/fmicb.2021.639266/fullmethanotrophmethanemethanolmethane monooxygenasemethanol dehydrogenase |
| spellingShingle | Hidehiro Ito Hidehiro Ito Kosei Yoshimori Masahito Ishikawa Katsutoshi Hori Toshiaki Kamachi Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant Frontiers in Microbiology methanotroph methane methanol methane monooxygenase methanol dehydrogenase |
| title | Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant |
| title_full | Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant |
| title_fullStr | Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant |
| title_full_unstemmed | Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant |
| title_short | Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant |
| title_sort | switching between methanol accumulation and cell growth by expression control of methanol dehydrogenase in methylosinus trichosporium ob3b mutant |
| topic | methanotroph methane methanol methane monooxygenase methanol dehydrogenase |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.639266/full |
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