Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression
Abstract Background Non-coding RNAs (ncRNAs) have been shown to regulate gene expression involved in tumor progression of multiple malignancies. Our previous studies indicated that large tumor suppressor kinase 1 (LATS1), a core part of Hippo signaling pathway, functions as a tumor suppressor in gas...
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| Format: | Article |
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BMC
2017-09-01
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| Series: | Molecular Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12943-017-0719-3 |
| _version_ | 1818876103838662656 |
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| author | Jing Zhang Hui Liu Lidan Hou Ge Wang Rui Zhang Yanxia Huang Xiaoyu Chen Jinshui Zhu |
| author_facet | Jing Zhang Hui Liu Lidan Hou Ge Wang Rui Zhang Yanxia Huang Xiaoyu Chen Jinshui Zhu |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | Abstract Background Non-coding RNAs (ncRNAs) have been shown to regulate gene expression involved in tumor progression of multiple malignancies. Our previous studies indicated that large tumor suppressor kinase 1 (LATS1), a core part of Hippo signaling pathway, functions as a tumor suppressor in gastric cancer (GC). But, the underlying molecular mechanisms by which ncRNAs modulate LATS1 expression in GC remain undetermined. Methods The correlation of LATS1 and has-miR-424-5p (miR-424) expression with clinicopathological characteristics and prognosis of GC patients was analyzed by TCGA RNA-sequencing data. A novel circular RNA_LARP4 (circLARP4) was identified to sponge miR-424 by circRNA expression profile and bioinformatic analysis. The binding site between miR-424 and LATS1 or circLARP4 was verified using dual luciferase assay and RNA immunoprecipitation (RIP) assay. The expression and localization of circLARP4 in GC tissues were investigated by fluorescence in situ hybridization (FISH). MTT, colony formation, Transwell and EdU assays were performed to assess the effects of miR-424 or circLARP4 on cell proliferation and invasion. Results Increased miR-424 expression or decreased LATS1 expression was associated with pathological stage and unfavorable prognosis of GC patients. Ectopic expression of miR-424 promoted proliferation and invasion of GC cells by targeting LATS1 gene. Furthermore, circLARP4 was mainly localized in the cytoplasm and inhibited biological behaviors of GC cells by sponging miR-424. The expression of circLARP4 was downregulated in GC tissues and represented an independent prognostic factor for overall survival of GC patients. Conclusion circLARP4 may act as a novel tumor suppressive factor and a potential biomarker in GC. |
| first_indexed | 2024-12-19T13:37:04Z |
| format | Article |
| id | doaj.art-2f07cb53fce341cdb1d2f21d80bc2b9c |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-19T13:37:04Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-2f07cb53fce341cdb1d2f21d80bc2b9c2022-12-21T20:19:10ZengBMCMolecular Cancer1476-45982017-09-0116111610.1186/s12943-017-0719-3Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expressionJing Zhang0Hui Liu1Lidan Hou2Ge Wang3Rui Zhang4Yanxia Huang5Xiaoyu Chen6Jinshui Zhu7Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Gastroenterology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalDepartment of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth People’s HospitalAbstract Background Non-coding RNAs (ncRNAs) have been shown to regulate gene expression involved in tumor progression of multiple malignancies. Our previous studies indicated that large tumor suppressor kinase 1 (LATS1), a core part of Hippo signaling pathway, functions as a tumor suppressor in gastric cancer (GC). But, the underlying molecular mechanisms by which ncRNAs modulate LATS1 expression in GC remain undetermined. Methods The correlation of LATS1 and has-miR-424-5p (miR-424) expression with clinicopathological characteristics and prognosis of GC patients was analyzed by TCGA RNA-sequencing data. A novel circular RNA_LARP4 (circLARP4) was identified to sponge miR-424 by circRNA expression profile and bioinformatic analysis. The binding site between miR-424 and LATS1 or circLARP4 was verified using dual luciferase assay and RNA immunoprecipitation (RIP) assay. The expression and localization of circLARP4 in GC tissues were investigated by fluorescence in situ hybridization (FISH). MTT, colony formation, Transwell and EdU assays were performed to assess the effects of miR-424 or circLARP4 on cell proliferation and invasion. Results Increased miR-424 expression or decreased LATS1 expression was associated with pathological stage and unfavorable prognosis of GC patients. Ectopic expression of miR-424 promoted proliferation and invasion of GC cells by targeting LATS1 gene. Furthermore, circLARP4 was mainly localized in the cytoplasm and inhibited biological behaviors of GC cells by sponging miR-424. The expression of circLARP4 was downregulated in GC tissues and represented an independent prognostic factor for overall survival of GC patients. Conclusion circLARP4 may act as a novel tumor suppressive factor and a potential biomarker in GC.http://link.springer.com/article/10.1186/s12943-017-0719-3Circular RNA_LARP4miR-424-5pLATS1InvasionGastric cancer |
| spellingShingle | Jing Zhang Hui Liu Lidan Hou Ge Wang Rui Zhang Yanxia Huang Xiaoyu Chen Jinshui Zhu Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression Molecular Cancer Circular RNA_LARP4 miR-424-5p LATS1 Invasion Gastric cancer |
| title | Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression |
| title_full | Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression |
| title_fullStr | Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression |
| title_full_unstemmed | Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression |
| title_short | Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression |
| title_sort | circular rna larp4 inhibits cell proliferation and invasion of gastric cancer by sponging mir 424 5p and regulating lats1 expression |
| topic | Circular RNA_LARP4 miR-424-5p LATS1 Invasion Gastric cancer |
| url | http://link.springer.com/article/10.1186/s12943-017-0719-3 |
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