Induced Genetic Variations in Stomatal Density and Size of Rice Strongly Affects Water Use Efficiency and Responses to Drought Stresses
Rice (Oryza sativa L.) is an important food crop relied upon by billions of people worldwide. However, with increasing pressure from climate change and rapid population growth, cultivation is very water-intensive. Therefore, it is critical to produce rice that is high-yielding and genetically more w...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.801706/full |
| _version_ | 1811255334900072448 |
|---|---|
| author | Mutiara K. Pitaloka Robert S. Caine Christopher Hepworth Emily L. Harrison Jennifer Sloan Cattleya Chutteang Chutima Phunthong Rangsan Nongngok Theerayut Toojinda Siriphat Ruengphayak Siwaret Arikit Siwaret Arikit Julie E. Gray Apichart Vanavichit Apichart Vanavichit Apichart Vanavichit |
| author_facet | Mutiara K. Pitaloka Robert S. Caine Christopher Hepworth Emily L. Harrison Jennifer Sloan Cattleya Chutteang Chutima Phunthong Rangsan Nongngok Theerayut Toojinda Siriphat Ruengphayak Siwaret Arikit Siwaret Arikit Julie E. Gray Apichart Vanavichit Apichart Vanavichit Apichart Vanavichit |
| author_sort | Mutiara K. Pitaloka |
| collection | DOAJ |
| description | Rice (Oryza sativa L.) is an important food crop relied upon by billions of people worldwide. However, with increasing pressure from climate change and rapid population growth, cultivation is very water-intensive. Therefore, it is critical to produce rice that is high-yielding and genetically more water-use efficient. Here, using the stabilized fast-neutron mutagenized population of Jao Hom Nin (JHN) - a popular purple rice cultivar - we microscopically examined hundreds of flag leaves to identify four stomatal model mutants with either high density (HD) or low density (LD) stomata, and small-sized (SS) or large-sized (LS) stomata. With similar genetic background and uniformity, the stomatal model mutants were used to understand the role of stomatal variants on physiological responses to abiotic stress. Our results show that SS and HD respond better to increasing CO2 concentration and HD has higher stomatal conductance (gs) compared to the other stomatal model mutants, although the effects on gas exchange or overall plant performance were small under greenhouse conditions. In addition, the results of our drought experiments suggest that LD and SS can better adapt to restricted water conditions, and LD showed higher water use efficiency (WUE) and biomass/plant than other stomatal model mutants under long-term restricted water treatment. Finally, our study suggests that reducing stomata density and size may play a promising role for further work on developing a climate-ready rice variety to adapt to drought and heat stress. We propose that low stomata density and small size have high potential as genetic donors for improving WUE in climate-ready rice. |
| first_indexed | 2024-04-12T17:22:17Z |
| format | Article |
| id | doaj.art-2f0b44fd32e1483596a01fe198654e84 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T17:22:17Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2f0b44fd32e1483596a01fe198654e842022-12-22T03:23:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.801706801706Induced Genetic Variations in Stomatal Density and Size of Rice Strongly Affects Water Use Efficiency and Responses to Drought StressesMutiara K. Pitaloka0Robert S. Caine1Christopher Hepworth2Emily L. Harrison3Jennifer Sloan4Cattleya Chutteang5Chutima Phunthong6Rangsan Nongngok7Theerayut Toojinda8Siriphat Ruengphayak9Siwaret Arikit10Siwaret Arikit11Julie E. Gray12Apichart Vanavichit13Apichart Vanavichit14Apichart Vanavichit15Faculty of Agriculture Kamphangsaen, Kasetsart University, Nakhon Pathom, ThailandPlants, Photosynthesis and Soil, School of Biosciences, University of Sheffield, Sheffield, United KingdomDepartment of Agronomy, Faculty of Agriculture Kamphangsaen, Kasetsart University, Nakhon Pathom, ThailandPlants, Photosynthesis and Soil, School of Biosciences, University of Sheffield, Sheffield, United KingdomPlants, Photosynthesis and Soil, School of Biosciences, University of Sheffield, Sheffield, United KingdomDepartment of Agronomy, Faculty of Agriculture Kamphangsaen, Kasetsart University, Nakhon Pathom, ThailandRice Science Center, Kasetsart University, Nakhon Pathom, ThailandRice Science Center, Kasetsart University, Nakhon Pathom, ThailandNational Center of Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang, ThailandRice Science Center, Kasetsart University, Nakhon Pathom, ThailandDepartment of Agronomy, Faculty of Agriculture Kamphangsaen, Kasetsart University, Nakhon Pathom, ThailandRice Science Center, Kasetsart University, Nakhon Pathom, ThailandPlants, Photosynthesis and Soil, School of Biosciences, University of Sheffield, Sheffield, United KingdomDepartment of Agronomy, Faculty of Agriculture Kamphangsaen, Kasetsart University, Nakhon Pathom, ThailandRice Science Center, Kasetsart University, Nakhon Pathom, ThailandNational Center of Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang, ThailandRice (Oryza sativa L.) is an important food crop relied upon by billions of people worldwide. However, with increasing pressure from climate change and rapid population growth, cultivation is very water-intensive. Therefore, it is critical to produce rice that is high-yielding and genetically more water-use efficient. Here, using the stabilized fast-neutron mutagenized population of Jao Hom Nin (JHN) - a popular purple rice cultivar - we microscopically examined hundreds of flag leaves to identify four stomatal model mutants with either high density (HD) or low density (LD) stomata, and small-sized (SS) or large-sized (LS) stomata. With similar genetic background and uniformity, the stomatal model mutants were used to understand the role of stomatal variants on physiological responses to abiotic stress. Our results show that SS and HD respond better to increasing CO2 concentration and HD has higher stomatal conductance (gs) compared to the other stomatal model mutants, although the effects on gas exchange or overall plant performance were small under greenhouse conditions. In addition, the results of our drought experiments suggest that LD and SS can better adapt to restricted water conditions, and LD showed higher water use efficiency (WUE) and biomass/plant than other stomatal model mutants under long-term restricted water treatment. Finally, our study suggests that reducing stomata density and size may play a promising role for further work on developing a climate-ready rice variety to adapt to drought and heat stress. We propose that low stomata density and small size have high potential as genetic donors for improving WUE in climate-ready rice.https://www.frontiersin.org/articles/10.3389/fpls.2022.801706/fullmutantstomataricedroughtWUE |
| spellingShingle | Mutiara K. Pitaloka Robert S. Caine Christopher Hepworth Emily L. Harrison Jennifer Sloan Cattleya Chutteang Chutima Phunthong Rangsan Nongngok Theerayut Toojinda Siriphat Ruengphayak Siwaret Arikit Siwaret Arikit Julie E. Gray Apichart Vanavichit Apichart Vanavichit Apichart Vanavichit Induced Genetic Variations in Stomatal Density and Size of Rice Strongly Affects Water Use Efficiency and Responses to Drought Stresses Frontiers in Plant Science mutant stomata rice drought WUE |
| title | Induced Genetic Variations in Stomatal Density and Size of Rice Strongly Affects Water Use Efficiency and Responses to Drought Stresses |
| title_full | Induced Genetic Variations in Stomatal Density and Size of Rice Strongly Affects Water Use Efficiency and Responses to Drought Stresses |
| title_fullStr | Induced Genetic Variations in Stomatal Density and Size of Rice Strongly Affects Water Use Efficiency and Responses to Drought Stresses |
| title_full_unstemmed | Induced Genetic Variations in Stomatal Density and Size of Rice Strongly Affects Water Use Efficiency and Responses to Drought Stresses |
| title_short | Induced Genetic Variations in Stomatal Density and Size of Rice Strongly Affects Water Use Efficiency and Responses to Drought Stresses |
| title_sort | induced genetic variations in stomatal density and size of rice strongly affects water use efficiency and responses to drought stresses |
| topic | mutant stomata rice drought WUE |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.801706/full |
| work_keys_str_mv | AT mutiarakpitaloka inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT robertscaine inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT christopherhepworth inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT emilylharrison inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT jennifersloan inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT cattleyachutteang inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT chutimaphunthong inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT rangsannongngok inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT theerayuttoojinda inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT siriphatruengphayak inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT siwaretarikit inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT siwaretarikit inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT julieegray inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT apichartvanavichit inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT apichartvanavichit inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses AT apichartvanavichit inducedgeneticvariationsinstomataldensityandsizeofricestronglyaffectswateruseefficiencyandresponsestodroughtstresses |