Recurrent Vulvovaginal Candidiasis: Could It Be Related to Cell-Mediated Immunity Defect in Response to Candida Antigen?
Background Recurrent vulvovaginal candidiasis (RVVC) is a common cause of morbidity affecting millions of women worldwide. Patients with RVVC are thought to have an underlying immunologic defect. This study has been established to evaluate cell-mediated immunity defect in response to candida antigen...
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| Format: | Article |
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Royan Institute (ACECR), Tehran
2017-10-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45427_998d7d4316fd4218102553a81171cbbf.pdf |
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| author | Zahra Talaei Saba Sheikhbahaei Vajihe Ostadi Mazdak Ganjalikhani Hakemi Mohsen Meidani Elham Naghshineh Majid Yaran Alireza Emami Naeini Roya Sherkat |
| author_facet | Zahra Talaei Saba Sheikhbahaei Vajihe Ostadi Mazdak Ganjalikhani Hakemi Mohsen Meidani Elham Naghshineh Majid Yaran Alireza Emami Naeini Roya Sherkat |
| author_sort | Zahra Talaei |
| collection | DOAJ |
| description | Background Recurrent vulvovaginal candidiasis (RVVC) is a common cause of morbidity affecting millions of women worldwide. Patients with RVVC are thought to have an underlying immunologic defect. This study has been established to evaluate cell-mediated immunity defect in response to candida antigen in RVVC cases. Materials and Methods Our cross-sectional study was performed in 3 groups of RVVC patients (cases), healthy individuals (control I) and known cases of chronic mucocutaneous candidiasis (CMC) (control II). Patients who met the inclusion criteria of RVVC were selected consecutively and were allocated in the case group. Peripheral blood mononuclear cells were isolated and labeled with CFSE and proliferation rate was measured in exposure to candida antigen via flow cytometry. Results T lymphocyte proliferation in response to candida was significantly lower in RVVC cases (n=24) and CMC patients (n=7) compared to healthy individuals (n=20, P < 0.001), but no statistically significant difference was seen between cases and control II group (P > 0.05). Family history of primary immunodeficiency diseases (PID) differed significantly among groups (P=0.01), RVVC patients has family history of PID more than control I (29.2 vs. 0%, P=0.008) but not statistically different from CMC patients (29.2 vs. 42.9%, P > 0.05). Prevalence of atopy was greater in RVVC cases compared to healthy individuals (41.3 vs. 15%, P=0.054). Lymphoproliferative activity and vaginal symptoms were significantly different among RVVC cases with and without allergy (P=0.01, P=0.02). Conclusion Our findings revealed that T cells do not actively proliferate in response to Candida antigen in some RVVC cases. So it is concluded that patients with cell-mediated immunity defect are more susceptible to recurrent fungal infections of vulva and vagina. Nonetheless, some other cases of RVVC showed normal function of T cells. Further evaluations showed that these patients suffer from atopy. It is hypothesized that higher frequency of VVC in patients with history of atopy might be due to allergic response in mucocutaneous membranes rather than a functional impairment in immune system components. |
| first_indexed | 2024-12-19T12:01:33Z |
| format | Article |
| id | doaj.art-2f0fbe2a18ab456e9f948759a5b00bef |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-19T12:01:33Z |
| publishDate | 2017-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-2f0fbe2a18ab456e9f948759a5b00bef2022-12-21T20:22:27ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782017-10-0111313414110.22074/ijfs.2017.488345427Recurrent Vulvovaginal Candidiasis: Could It Be Related to Cell-Mediated Immunity Defect in Response to Candida Antigen?Zahra Talaei0Saba Sheikhbahaei1Vajihe Ostadi2Mazdak Ganjalikhani Hakemi3Mohsen Meidani4Elham Naghshineh5Majid Yaran6Alireza Emami Naeini7Roya Sherkat8Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranAcquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranAcquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranCellular and Molecular Immunology Research Center, Isfahan University of Medical Sciences, Isfahan, IranInfectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran4Department of Obstetrics Gynecology, Isfahan University of Medical Sciences, Isfahan, IranAcquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranAcquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranAcquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranBackground Recurrent vulvovaginal candidiasis (RVVC) is a common cause of morbidity affecting millions of women worldwide. Patients with RVVC are thought to have an underlying immunologic defect. This study has been established to evaluate cell-mediated immunity defect in response to candida antigen in RVVC cases. Materials and Methods Our cross-sectional study was performed in 3 groups of RVVC patients (cases), healthy individuals (control I) and known cases of chronic mucocutaneous candidiasis (CMC) (control II). Patients who met the inclusion criteria of RVVC were selected consecutively and were allocated in the case group. Peripheral blood mononuclear cells were isolated and labeled with CFSE and proliferation rate was measured in exposure to candida antigen via flow cytometry. Results T lymphocyte proliferation in response to candida was significantly lower in RVVC cases (n=24) and CMC patients (n=7) compared to healthy individuals (n=20, P < 0.001), but no statistically significant difference was seen between cases and control II group (P > 0.05). Family history of primary immunodeficiency diseases (PID) differed significantly among groups (P=0.01), RVVC patients has family history of PID more than control I (29.2 vs. 0%, P=0.008) but not statistically different from CMC patients (29.2 vs. 42.9%, P > 0.05). Prevalence of atopy was greater in RVVC cases compared to healthy individuals (41.3 vs. 15%, P=0.054). Lymphoproliferative activity and vaginal symptoms were significantly different among RVVC cases with and without allergy (P=0.01, P=0.02). Conclusion Our findings revealed that T cells do not actively proliferate in response to Candida antigen in some RVVC cases. So it is concluded that patients with cell-mediated immunity defect are more susceptible to recurrent fungal infections of vulva and vagina. Nonetheless, some other cases of RVVC showed normal function of T cells. Further evaluations showed that these patients suffer from atopy. It is hypothesized that higher frequency of VVC in patients with history of atopy might be due to allergic response in mucocutaneous membranes rather than a functional impairment in immune system components.http://www.ijfs.ir/article_45427_998d7d4316fd4218102553a81171cbbf.pdfallergycell mediated immunityvulvovaginal candidiasisatopy |
| spellingShingle | Zahra Talaei Saba Sheikhbahaei Vajihe Ostadi Mazdak Ganjalikhani Hakemi Mohsen Meidani Elham Naghshineh Majid Yaran Alireza Emami Naeini Roya Sherkat Recurrent Vulvovaginal Candidiasis: Could It Be Related to Cell-Mediated Immunity Defect in Response to Candida Antigen? International Journal of Fertility and Sterility allergy cell mediated immunity vulvovaginal candidiasis atopy |
| title | Recurrent Vulvovaginal Candidiasis: Could It Be Related to Cell-Mediated Immunity Defect in Response to Candida Antigen? |
| title_full | Recurrent Vulvovaginal Candidiasis: Could It Be Related to Cell-Mediated Immunity Defect in Response to Candida Antigen? |
| title_fullStr | Recurrent Vulvovaginal Candidiasis: Could It Be Related to Cell-Mediated Immunity Defect in Response to Candida Antigen? |
| title_full_unstemmed | Recurrent Vulvovaginal Candidiasis: Could It Be Related to Cell-Mediated Immunity Defect in Response to Candida Antigen? |
| title_short | Recurrent Vulvovaginal Candidiasis: Could It Be Related to Cell-Mediated Immunity Defect in Response to Candida Antigen? |
| title_sort | recurrent vulvovaginal candidiasis could it be related to cell mediated immunity defect in response to candida antigen |
| topic | allergy cell mediated immunity vulvovaginal candidiasis atopy |
| url | http://www.ijfs.ir/article_45427_998d7d4316fd4218102553a81171cbbf.pdf |
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