A microfabricated multi-compartment device for neuron and Schwann cell differentiation
Abstract Understanding the complex communication between different cell populations and their interaction with the microenvironment in the central and peripheral nervous systems is fundamental in neuroscience research. The development of appropriate in vitro approaches and tools, able to selectively...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-86300-4 |
| _version_ | 1818845430664921088 |
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| author | Eleonora De Vitis Velia La Pesa Francesca Gervaso Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Alessandro Polini |
| author_facet | Eleonora De Vitis Velia La Pesa Francesca Gervaso Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Alessandro Polini |
| author_sort | Eleonora De Vitis |
| collection | DOAJ |
| description | Abstract Understanding the complex communication between different cell populations and their interaction with the microenvironment in the central and peripheral nervous systems is fundamental in neuroscience research. The development of appropriate in vitro approaches and tools, able to selectively analyze and/or probe specific cells and cell portions (e.g., axons and cell bodies in neurons), driving their differentiation into specific cell phenotypes, has become therefore crucial in this direction. Here we report a multi-compartment microfluidic device where up to three different cell populations can be cultured in a fluidically independent circuit. The device allows cell migration across the compartments and their differentiation. We showed that an accurate choice of the device geometrical features and cell culture parameters allows to (1) maximize cell adhesion and proliferation of neuron-like human cells (SH-SY5Y cells), (2) control the inter-compartment cell migration of neuron and Schwann cells, (3) perform long-term cell culture studies in which both SH-SY5Y cells and primary rat Schwann cells can be differentiated towards specific phenotypes. These results can lead to a plethora of in vitro co-culture studies in the neuroscience research field, where tuning and investigating cell–cell and cell–microenvironment interactions are essential. |
| first_indexed | 2024-12-19T05:29:32Z |
| format | Article |
| id | doaj.art-2f10b8d2d78c4303b2ba0c0c109f9291 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:29:32Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2f10b8d2d78c4303b2ba0c0c109f92912022-12-21T20:34:16ZengNature PortfolioScientific Reports2045-23222021-03-0111111210.1038/s41598-021-86300-4A microfabricated multi-compartment device for neuron and Schwann cell differentiationEleonora De Vitis0Velia La Pesa1Francesca Gervaso2Alessandro Romano3Angelo Quattrini4Giuseppe Gigli5Lorenzo Moroni6Alessandro Polini7CNR NANOTEC – Institute of NanotechnologyDivision of Neuroscience, Institute of Experimental Neurology, IRCCS San Raffaele Scientific InstituteCNR NANOTEC – Institute of NanotechnologyDivision of Neuroscience, Institute of Experimental Neurology, IRCCS San Raffaele Scientific InstituteDivision of Neuroscience, Institute of Experimental Neurology, IRCCS San Raffaele Scientific InstituteCNR NANOTEC – Institute of NanotechnologyCNR NANOTEC – Institute of NanotechnologyCNR NANOTEC – Institute of NanotechnologyAbstract Understanding the complex communication between different cell populations and their interaction with the microenvironment in the central and peripheral nervous systems is fundamental in neuroscience research. The development of appropriate in vitro approaches and tools, able to selectively analyze and/or probe specific cells and cell portions (e.g., axons and cell bodies in neurons), driving their differentiation into specific cell phenotypes, has become therefore crucial in this direction. Here we report a multi-compartment microfluidic device where up to three different cell populations can be cultured in a fluidically independent circuit. The device allows cell migration across the compartments and their differentiation. We showed that an accurate choice of the device geometrical features and cell culture parameters allows to (1) maximize cell adhesion and proliferation of neuron-like human cells (SH-SY5Y cells), (2) control the inter-compartment cell migration of neuron and Schwann cells, (3) perform long-term cell culture studies in which both SH-SY5Y cells and primary rat Schwann cells can be differentiated towards specific phenotypes. These results can lead to a plethora of in vitro co-culture studies in the neuroscience research field, where tuning and investigating cell–cell and cell–microenvironment interactions are essential.https://doi.org/10.1038/s41598-021-86300-4 |
| spellingShingle | Eleonora De Vitis Velia La Pesa Francesca Gervaso Alessandro Romano Angelo Quattrini Giuseppe Gigli Lorenzo Moroni Alessandro Polini A microfabricated multi-compartment device for neuron and Schwann cell differentiation Scientific Reports |
| title | A microfabricated multi-compartment device for neuron and Schwann cell differentiation |
| title_full | A microfabricated multi-compartment device for neuron and Schwann cell differentiation |
| title_fullStr | A microfabricated multi-compartment device for neuron and Schwann cell differentiation |
| title_full_unstemmed | A microfabricated multi-compartment device for neuron and Schwann cell differentiation |
| title_short | A microfabricated multi-compartment device for neuron and Schwann cell differentiation |
| title_sort | microfabricated multi compartment device for neuron and schwann cell differentiation |
| url | https://doi.org/10.1038/s41598-021-86300-4 |
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