<i>Thymus algeriensis</i> and <i>Thymus fontanesii</i>: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison
This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of <i>Thymus algeriensis and Thymus fontanesii</i> (Lamiaceae). Thirty-five secondary metabolites wer...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/4/599 |
| _version_ | 1797570746039402496 |
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| author | Mansour Sobeh Samar Rezq Mohammed Cheurfa Mohamed A.O. Abdelfattah Rasha M.H. Rashied Assem M. El-Shazly Abdelaziz Yasri Michael Wink Mona F. Mahmoud |
| author_facet | Mansour Sobeh Samar Rezq Mohammed Cheurfa Mohamed A.O. Abdelfattah Rasha M.H. Rashied Assem M. El-Shazly Abdelaziz Yasri Michael Wink Mona F. Mahmoud |
| author_sort | Mansour Sobeh |
| collection | DOAJ |
| description | This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of <i>Thymus algeriensis and Thymus fontanesii</i> (Lamiaceae). Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the <i>T. algeriensis</i> extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in <i>T. fontanesii</i> extract. Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway. In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts. Results revealed that both studied <i>Thymus</i> species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects. They showed to be a more potent antioxidant than ascorbic acid and more selective against cyclooxygenase (COX-2) than diclofenac and indomethacin. Relatively, the <i>T. fontanesii</i> extract was more potent as COX-2 inhibitor than <i>T. algeriensis</i>. In conclusion, <i>Thymus algeriensis</i> and <i>Thymus fontanesii</i> may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders. |
| first_indexed | 2024-03-10T20:29:45Z |
| format | Article |
| id | doaj.art-2f13d524f9304d079ea114ca72526ed6 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T20:29:45Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-2f13d524f9304d079ea114ca72526ed62023-11-19T21:28:14ZengMDPI AGBiomolecules2218-273X2020-04-0110459910.3390/biom10040599<i>Thymus algeriensis</i> and <i>Thymus fontanesii</i>: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive ComparisonMansour Sobeh0Samar Rezq1Mohammed Cheurfa2Mohamed A.O. Abdelfattah3Rasha M.H. Rashied4Assem M. El-Shazly5Abdelaziz Yasri6Michael Wink7Mona F. Mahmoud8AgroBioSciences Research Division, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, Ben-Guerir 43150, MoroccoDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartement of Biology, Faculty of Nature, Life and Earth Sciences, University of Djillali Bounaama, Khemis Miliana Road Teniet Elhad, Khemis Miliana 44225, AlgeriaCollege of Engineering and Technology, American University of the Middle East, KuwaitDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptAgroBioSciences Research Division, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, Ben-Guerir 43150, MoroccoInstitute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, GermanyDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptThis study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of <i>Thymus algeriensis and Thymus fontanesii</i> (Lamiaceae). Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the <i>T. algeriensis</i> extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in <i>T. fontanesii</i> extract. Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway. In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts. Results revealed that both studied <i>Thymus</i> species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects. They showed to be a more potent antioxidant than ascorbic acid and more selective against cyclooxygenase (COX-2) than diclofenac and indomethacin. Relatively, the <i>T. fontanesii</i> extract was more potent as COX-2 inhibitor than <i>T. algeriensis</i>. In conclusion, <i>Thymus algeriensis</i> and <i>Thymus fontanesii</i> may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.https://www.mdpi.com/2218-273X/10/4/599<i>Thymus algeriensis</i><i>Thymus fontanesii</i>antioxidant activityanti-inflammatoryantipyreticanalgesic |
| spellingShingle | Mansour Sobeh Samar Rezq Mohammed Cheurfa Mohamed A.O. Abdelfattah Rasha M.H. Rashied Assem M. El-Shazly Abdelaziz Yasri Michael Wink Mona F. Mahmoud <i>Thymus algeriensis</i> and <i>Thymus fontanesii</i>: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison Biomolecules <i>Thymus algeriensis</i> <i>Thymus fontanesii</i> antioxidant activity anti-inflammatory antipyretic analgesic |
| title | <i>Thymus algeriensis</i> and <i>Thymus fontanesii</i>: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison |
| title_full | <i>Thymus algeriensis</i> and <i>Thymus fontanesii</i>: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison |
| title_fullStr | <i>Thymus algeriensis</i> and <i>Thymus fontanesii</i>: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison |
| title_full_unstemmed | <i>Thymus algeriensis</i> and <i>Thymus fontanesii</i>: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison |
| title_short | <i>Thymus algeriensis</i> and <i>Thymus fontanesii</i>: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison |
| title_sort | i thymus algeriensis i and i thymus fontanesii i chemical composition in vivo antiinflammatory pain killing and antipyretic activities a comprehensive comparison |
| topic | <i>Thymus algeriensis</i> <i>Thymus fontanesii</i> antioxidant activity anti-inflammatory antipyretic analgesic |
| url | https://www.mdpi.com/2218-273X/10/4/599 |
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