Protective effects of menthol against sepsis-induced hepatic injury: Role of mediators of hepatic inflammation, apoptosis, and regeneration
Liver dysfunction in sepsis is a major complication that amplifies multiple organ failure and increases the risk of death. Inflammation and oxidative stress are the main mediators in the pathophysiology of sepsis. Therefore, we investigated the role of menthol, a natural antioxidant, against sepsis-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.952337/full |
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| author | Asmaa I. Matouk Mahmoud El-Daly Heba A. Habib Shaymaa Senousy Sara Mohamed Naguib Abdel Hafez AlShaimaa W. Kasem Waleed Hassan Almalki Abdulaziz Alzahrani Ahmed Alshehri Al-Shaimaa F. Ahmed |
| author_facet | Asmaa I. Matouk Mahmoud El-Daly Heba A. Habib Shaymaa Senousy Sara Mohamed Naguib Abdel Hafez AlShaimaa W. Kasem Waleed Hassan Almalki Abdulaziz Alzahrani Ahmed Alshehri Al-Shaimaa F. Ahmed |
| author_sort | Asmaa I. Matouk |
| collection | DOAJ |
| description | Liver dysfunction in sepsis is a major complication that amplifies multiple organ failure and increases the risk of death. Inflammation and oxidative stress are the main mediators in the pathophysiology of sepsis. Therefore, we investigated the role of menthol, a natural antioxidant, against sepsis-induced liver injury in female Wistar rats. Sepsis was induced by cecal ligation and puncture (CLP). Menthol (100 mg/kg) was given intragastric 2 h after CLP. Blood samples and liver tissues were collected 24 h after surgery. Menthol significantly (p < 0.05) attenuated the sepsis-induced elevation in serum liver enzymes and improved the hepatic histopathological changes. Menthol treatment significantly (p < 0.05) decreased hepatic levels of tumor necrosis factor-alpha, malondialdehyde, total nitrite, and cleaved caspase-3. It restored the hepatic levels of superoxide dismutase and reduced glutathione. Additionally, menthol significantly (p < 0.05) increased hepatic levels of B-cell lymphoma 2 (Bcl-2); an anti-apoptotic factor, and proliferating cell nuclear antigen (PCNA), a biomarker of regeneration and survival. Our results showed the therapeutic potential of menthol against liver injury induced by sepsis. |
| first_indexed | 2024-04-13T17:56:49Z |
| format | Article |
| id | doaj.art-2f14aa5e4f064954b8342421b878a85e |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T17:56:49Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-2f14aa5e4f064954b8342421b878a85e2022-12-22T02:36:27ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-08-011310.3389/fphar.2022.952337952337Protective effects of menthol against sepsis-induced hepatic injury: Role of mediators of hepatic inflammation, apoptosis, and regenerationAsmaa I. Matouk0Mahmoud El-Daly1Heba A. Habib2Shaymaa Senousy3Sara Mohamed Naguib Abdel Hafez4AlShaimaa W. Kasem5Waleed Hassan Almalki6Abdulaziz Alzahrani7Ahmed Alshehri8Al-Shaimaa F. Ahmed9Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minya, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minya, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minya, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minya, EgyptDepartment of Pathology, Faculty of Medicine, Minia University, Minya, EgyptDepartment of Histology and Cell Biology, Faculty of Medicine, Minia University, Minya, EgyptDepartment of Pharmacology and Toxicology, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Clinical Pharmacy, AlBaha University, Al Bahah, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Clinical Pharmacy, AlBaha University, Al Bahah, Saudi ArabiaDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minya, EgyptLiver dysfunction in sepsis is a major complication that amplifies multiple organ failure and increases the risk of death. Inflammation and oxidative stress are the main mediators in the pathophysiology of sepsis. Therefore, we investigated the role of menthol, a natural antioxidant, against sepsis-induced liver injury in female Wistar rats. Sepsis was induced by cecal ligation and puncture (CLP). Menthol (100 mg/kg) was given intragastric 2 h after CLP. Blood samples and liver tissues were collected 24 h after surgery. Menthol significantly (p < 0.05) attenuated the sepsis-induced elevation in serum liver enzymes and improved the hepatic histopathological changes. Menthol treatment significantly (p < 0.05) decreased hepatic levels of tumor necrosis factor-alpha, malondialdehyde, total nitrite, and cleaved caspase-3. It restored the hepatic levels of superoxide dismutase and reduced glutathione. Additionally, menthol significantly (p < 0.05) increased hepatic levels of B-cell lymphoma 2 (Bcl-2); an anti-apoptotic factor, and proliferating cell nuclear antigen (PCNA), a biomarker of regeneration and survival. Our results showed the therapeutic potential of menthol against liver injury induced by sepsis.https://www.frontiersin.org/articles/10.3389/fphar.2022.952337/fullclphepatoprotectiontNF-alphaPCNAapoptosis |
| spellingShingle | Asmaa I. Matouk Mahmoud El-Daly Heba A. Habib Shaymaa Senousy Sara Mohamed Naguib Abdel Hafez AlShaimaa W. Kasem Waleed Hassan Almalki Abdulaziz Alzahrani Ahmed Alshehri Al-Shaimaa F. Ahmed Protective effects of menthol against sepsis-induced hepatic injury: Role of mediators of hepatic inflammation, apoptosis, and regeneration Frontiers in Pharmacology clp hepatoprotection tNF-alpha PCNA apoptosis |
| title | Protective effects of menthol against sepsis-induced hepatic injury: Role of mediators of hepatic inflammation, apoptosis, and regeneration |
| title_full | Protective effects of menthol against sepsis-induced hepatic injury: Role of mediators of hepatic inflammation, apoptosis, and regeneration |
| title_fullStr | Protective effects of menthol against sepsis-induced hepatic injury: Role of mediators of hepatic inflammation, apoptosis, and regeneration |
| title_full_unstemmed | Protective effects of menthol against sepsis-induced hepatic injury: Role of mediators of hepatic inflammation, apoptosis, and regeneration |
| title_short | Protective effects of menthol against sepsis-induced hepatic injury: Role of mediators of hepatic inflammation, apoptosis, and regeneration |
| title_sort | protective effects of menthol against sepsis induced hepatic injury role of mediators of hepatic inflammation apoptosis and regeneration |
| topic | clp hepatoprotection tNF-alpha PCNA apoptosis |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.952337/full |
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