Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver.
The objective of this study is to determine the effect of Chinese wolfberry (Lycium barbarum) and Astragalus (Astragalus membranaceus) extract (WAE) on the antioxidant capacity of Tibetan pig liver, and discussed the regulatory effect of WAE on the liver antioxidant mechanism. Twelve healthy 120-day...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0245749 |
| _version_ | 1818727995526873088 |
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| author | Zhuang Hao Zhen Li Jinjin Huo Jiandong Li Fenghua Liu Peng Yin |
| author_facet | Zhuang Hao Zhen Li Jinjin Huo Jiandong Li Fenghua Liu Peng Yin |
| author_sort | Zhuang Hao |
| collection | DOAJ |
| description | The objective of this study is to determine the effect of Chinese wolfberry (Lycium barbarum) and Astragalus (Astragalus membranaceus) extract (WAE) on the antioxidant capacity of Tibetan pig liver, and discussed the regulatory effect of WAE on the liver antioxidant mechanism. Twelve healthy 120-day-old Tibetan black pigs (35±2 kg) were divided randomly into two groups. The WAE group was fed a basal diet supplemented with 1% WAE for 90 days. The control group was fed the same diet, but without the WAE. We found that liver superoxide dismutase 1 (SOD1) activity (P<0.05), total antioxidative capacity (T-AOC) (P<0.05), and catalase (CAT) activity (P<0.01) significantly increased in the WAE group compared with the control group; malondialdehyde (MDA) content decreased, but this was not significant (P >0.05). Transcriptome sequencing analysis detected 106 differentially expressed genes (DEGs) related to oxidative stress. GO enrichment analysis showed these DEGs were involved in the positive regulation of reactive oxygen metabolism and biosynthesis, process regulation, and regulation of the oxidative stress response. KEGG Pathway enrichment analysis showed they were enriched in the PI3K-Akt, AMPK, Rap1, and peroxisome signaling pathways. The expression levels of key peroxisome biosynthesis genes (e.g., PEX3 and PEX11B) and key antioxidant genes (e.g., CAT and SOD1) were significantly higher in the WAE group than in the control group. The PRDX1 and PRDX5 content also was significantly higher in the WAE group. This study showed that the WAE regulated the antioxidant and anti-stress ability of Tibetan pig liver through a "peroxisome antioxidant-oxidant stress" signaling pathway. |
| first_indexed | 2024-12-17T22:22:57Z |
| format | Article |
| id | doaj.art-2f15d528bb6c4896b71a1a463eeca90d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T22:22:57Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2f15d528bb6c4896b71a1a463eeca90d2022-12-21T21:30:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01161e024574910.1371/journal.pone.0245749Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver.Zhuang HaoZhen LiJinjin HuoJiandong LiFenghua LiuPeng YinThe objective of this study is to determine the effect of Chinese wolfberry (Lycium barbarum) and Astragalus (Astragalus membranaceus) extract (WAE) on the antioxidant capacity of Tibetan pig liver, and discussed the regulatory effect of WAE on the liver antioxidant mechanism. Twelve healthy 120-day-old Tibetan black pigs (35±2 kg) were divided randomly into two groups. The WAE group was fed a basal diet supplemented with 1% WAE for 90 days. The control group was fed the same diet, but without the WAE. We found that liver superoxide dismutase 1 (SOD1) activity (P<0.05), total antioxidative capacity (T-AOC) (P<0.05), and catalase (CAT) activity (P<0.01) significantly increased in the WAE group compared with the control group; malondialdehyde (MDA) content decreased, but this was not significant (P >0.05). Transcriptome sequencing analysis detected 106 differentially expressed genes (DEGs) related to oxidative stress. GO enrichment analysis showed these DEGs were involved in the positive regulation of reactive oxygen metabolism and biosynthesis, process regulation, and regulation of the oxidative stress response. KEGG Pathway enrichment analysis showed they were enriched in the PI3K-Akt, AMPK, Rap1, and peroxisome signaling pathways. The expression levels of key peroxisome biosynthesis genes (e.g., PEX3 and PEX11B) and key antioxidant genes (e.g., CAT and SOD1) were significantly higher in the WAE group than in the control group. The PRDX1 and PRDX5 content also was significantly higher in the WAE group. This study showed that the WAE regulated the antioxidant and anti-stress ability of Tibetan pig liver through a "peroxisome antioxidant-oxidant stress" signaling pathway.https://doi.org/10.1371/journal.pone.0245749 |
| spellingShingle | Zhuang Hao Zhen Li Jinjin Huo Jiandong Li Fenghua Liu Peng Yin Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver. PLoS ONE |
| title | Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver. |
| title_full | Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver. |
| title_fullStr | Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver. |
| title_full_unstemmed | Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver. |
| title_short | Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver. |
| title_sort | effects of chinese wolfberry and astragalus extract on the antioxidant capacity of tibetan pig liver |
| url | https://doi.org/10.1371/journal.pone.0245749 |
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