The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects
Background: Obesity is a complex disorder influenced by various genetic and environmental factors. It has been shown that gut microbiota, which colonizes gastrointestinal tract, has a substantial role as an environmental factor in the pathophysiology of obesity. Since the composition of gut microbio...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2020-05-01
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| Series: | Iranian Biomedical Journal |
| Subjects: | |
| Online Access: | http://ibj.pasteur.ac.ir/article-1-2985-en.html |
| _version_ | 1830486837197537280 |
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| author | Fateme Ettehad Marvasti Arfa Moshiri Mina Sadat Taghavi Soheil Riazi Majid Taati Seyedeh Fatemeh Sadati Azadeh Ghaheri Morteza Masoomi Farzam Vaziri Abolfazl Fateh Pejman Rohani Samira Tarashi Andrea Masotti Sara Ahmadi Badi Seyed Davar Siadat |
| author_facet | Fateme Ettehad Marvasti Arfa Moshiri Mina Sadat Taghavi Soheil Riazi Majid Taati Seyedeh Fatemeh Sadati Azadeh Ghaheri Morteza Masoomi Farzam Vaziri Abolfazl Fateh Pejman Rohani Samira Tarashi Andrea Masotti Sara Ahmadi Badi Seyed Davar Siadat |
| author_sort | Fateme Ettehad Marvasti |
| collection | DOAJ |
| description | Background: Obesity is a complex disorder influenced by various genetic and environmental factors. It has been shown that gut microbiota, which colonizes gastrointestinal tract, has a substantial role as an environmental factor in the pathophysiology of obesity. Since the composition of gut microbiota alters with regard to different criteria, such as ethnicity, geographical location, diet, lifestyle, age, and gender, we aimed to determine firmicutes/bacteroidetes (F/B) ratio and the abundance of important gut microbiota members, Akkermansia muciniphila, Faecalibacterium prausnitzii, Roseburia, Bifidobacterium, and Prevotella in Iranian obese and normal weight individuals, for the first time. Methods: In this study, 50 normal and 50 obese subjects were recruited and classified based on their BMI into normal weight and obese groups. Stool samples were collected. Following DNA extraction from the samples, quantitative PCR was conducted based on 16s rDNA universal primers. Finally, the correlation between the bacterial abundance and obesity was analyzed by statistical analyses. Results: We observed a significant increase of F/B ratio in the obese group, compared to the normal weight group (p = 0.002). Although A. muciniphila (p = 0.039) and Bifidobacterium (p = 0.049) abundance significantly decreased, the abundance of F. prausnitzii (p = 0.046) significantly elevated with BMI increase in the studied groups. Conclusion: Owing to the importance of the gut microbiota composition in obesity development, determination and targeted restoration of gut microbiota pattern could be valuable in the control and treatment of obesity in certain populations. |
| first_indexed | 2024-12-21T18:50:11Z |
| format | Article |
| id | doaj.art-2f169820e78444e1b2110774b2a5e041 |
| institution | Directory Open Access Journal |
| issn | 1028-852X 2008-823X |
| language | English |
| last_indexed | 2024-12-21T18:50:11Z |
| publishDate | 2020-05-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Iranian Biomedical Journal |
| spelling | doaj.art-2f169820e78444e1b2110774b2a5e0412022-12-21T18:53:46ZengPasteur Institute of IranIranian Biomedical Journal1028-852X2008-823X2020-05-01243148154The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian SubjectsFateme Ettehad Marvasti0Arfa Moshiri1Mina Sadat Taghavi2Soheil Riazi3Majid Taati4Seyedeh Fatemeh Sadati5Azadeh Ghaheri6Morteza Masoomi7Farzam Vaziri8Abolfazl Fateh9Pejman Rohani10Samira Tarashi11Andrea Masotti12Sara Ahmadi Badi13Seyed Davar Siadat14 Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran Research Centre for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran The Islamic Azad University, Science and Research Branch, Tehran, Iran The Islamic Azad University, Science and Research Branch, Tehran, Iran The Islamic Azad University, Science and Research Branch, Tehran, Iran The Islamic Azad University, Science and Research Branch, Tehran, Iran Departments of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran Department of Pediatric Gastroentrology and Hepatology, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran Research Laboratories, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran Microbiology Research Centre, Pasteur Institute of Iran, Tehran, Iran Background: Obesity is a complex disorder influenced by various genetic and environmental factors. It has been shown that gut microbiota, which colonizes gastrointestinal tract, has a substantial role as an environmental factor in the pathophysiology of obesity. Since the composition of gut microbiota alters with regard to different criteria, such as ethnicity, geographical location, diet, lifestyle, age, and gender, we aimed to determine firmicutes/bacteroidetes (F/B) ratio and the abundance of important gut microbiota members, Akkermansia muciniphila, Faecalibacterium prausnitzii, Roseburia, Bifidobacterium, and Prevotella in Iranian obese and normal weight individuals, for the first time. Methods: In this study, 50 normal and 50 obese subjects were recruited and classified based on their BMI into normal weight and obese groups. Stool samples were collected. Following DNA extraction from the samples, quantitative PCR was conducted based on 16s rDNA universal primers. Finally, the correlation between the bacterial abundance and obesity was analyzed by statistical analyses. Results: We observed a significant increase of F/B ratio in the obese group, compared to the normal weight group (p = 0.002). Although A. muciniphila (p = 0.039) and Bifidobacterium (p = 0.049) abundance significantly decreased, the abundance of F. prausnitzii (p = 0.046) significantly elevated with BMI increase in the studied groups. Conclusion: Owing to the importance of the gut microbiota composition in obesity development, determination and targeted restoration of gut microbiota pattern could be valuable in the control and treatment of obesity in certain populations.http://ibj.pasteur.ac.ir/article-1-2985-en.htmlbacteroidetesbifidobacteriumfaecalibacterium prausnitziifirmicutesmicrobiota |
| spellingShingle | Fateme Ettehad Marvasti Arfa Moshiri Mina Sadat Taghavi Soheil Riazi Majid Taati Seyedeh Fatemeh Sadati Azadeh Ghaheri Morteza Masoomi Farzam Vaziri Abolfazl Fateh Pejman Rohani Samira Tarashi Andrea Masotti Sara Ahmadi Badi Seyed Davar Siadat The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects Iranian Biomedical Journal bacteroidetes bifidobacterium faecalibacterium prausnitzii firmicutes microbiota |
| title | The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects |
| title_full | The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects |
| title_fullStr | The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects |
| title_full_unstemmed | The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects |
| title_short | The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects |
| title_sort | first report of differences in gut microbiota composition between obese and normal weight iranian subjects |
| topic | bacteroidetes bifidobacterium faecalibacterium prausnitzii firmicutes microbiota |
| url | http://ibj.pasteur.ac.ir/article-1-2985-en.html |
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