Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines
Hepatitis E virus (HEV) infection has been demonstrated in various animal species; those recognized as potential zoonotic reservoirs pose a considerable risk to public health. In Brazil, HEV-3 is the only genotype identified in humans and swine nationwide, in a colony-breeding cynomolgus monkey and,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/11/2743 |
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| author | Caroline Roberta Soares Salgado Aldaleia do Nascimento e Silva Igor Falco Arruda Patrícia Riddell Millar Maria Regina Reis Amendoeira Luciane Almeida Amado Leon Raffaella Bertoni Cavalcanti Teixeira Jorge Tiburcio Barbosa de Lima Flávia Löwen Levy Chalhoub Ana Maria Bispo de Filippis Ana Beatriz Monteiro Fonseca Jaqueline Mendes de Oliveira Marcelo Alves Pinto Andreza Soriano Figueiredo |
| author_facet | Caroline Roberta Soares Salgado Aldaleia do Nascimento e Silva Igor Falco Arruda Patrícia Riddell Millar Maria Regina Reis Amendoeira Luciane Almeida Amado Leon Raffaella Bertoni Cavalcanti Teixeira Jorge Tiburcio Barbosa de Lima Flávia Löwen Levy Chalhoub Ana Maria Bispo de Filippis Ana Beatriz Monteiro Fonseca Jaqueline Mendes de Oliveira Marcelo Alves Pinto Andreza Soriano Figueiredo |
| author_sort | Caroline Roberta Soares Salgado |
| collection | DOAJ |
| description | Hepatitis E virus (HEV) infection has been demonstrated in various animal species; those recognized as potential zoonotic reservoirs pose a considerable risk to public health. In Brazil, HEV-3 is the only genotype identified in humans and swine nationwide, in a colony-breeding cynomolgus monkey and, recently, in bovines and capybara. There is no information regarding HEV exposure in the equine population in Brazil. This study aimed to investigate anti-HEV antibodies and viral RNA in serum samples from horses slaughtered for meat export and those bred for sport/reproduction purposes. We used a commercially available ELISA kit modified to detect species-specific anti-HEV, using an anti-horse IgG-peroxidase conjugate and evaluating different cutoff formulas and assay precision. Serum samples (n = 257) were tested for anti-HEV IgG and HEV RNA by nested RT-PCR and RT-qPCR. The overall anti-HEV seroprevalence was 26.5% (68/257) without the detection of HEV RNA. Most municipalities (53.3%) and farms (58.8%) had positive horses. Animals slaughtered for human consumption had higher risk of HEV exposure (45.5%) than those bred for sports or reproduction (6.4%) (<i>p</i> < 0.0001). The statistical analysis revealed sex and breeding system as possible risk-associated factors. The first serological evidence of HEV circulation in Brazilian equines reinforces the need for the surveillance of HEV host expansion in a one-health approach. |
| first_indexed | 2024-03-09T16:34:59Z |
| format | Article |
| id | doaj.art-2f1872472bd34b4aa08186db6835f548 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:34:59Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2f1872472bd34b4aa08186db6835f5482023-11-24T14:57:06ZengMDPI AGMicroorganisms2076-26072023-11-011111274310.3390/microorganisms11112743Serological Evidence of Hepatitis E Virus Infection in Brazilian EquinesCaroline Roberta Soares Salgado0Aldaleia do Nascimento e Silva1Igor Falco Arruda2Patrícia Riddell Millar3Maria Regina Reis Amendoeira4Luciane Almeida Amado Leon5Raffaella Bertoni Cavalcanti Teixeira6Jorge Tiburcio Barbosa de Lima7Flávia Löwen Levy Chalhoub8Ana Maria Bispo de Filippis9Ana Beatriz Monteiro Fonseca10Jaqueline Mendes de Oliveira11Marcelo Alves Pinto12Andreza Soriano Figueiredo13Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Toxoplasmose e outras Protozooses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Toxoplasmose e outras Protozooses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Toxoplasmose e outras Protozooses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilClínica de Grandes Animais, Departamento de Medicina Veterinária, Universidade Federal de Viçosa—UFV, Viçosa 36570-900, MG, BrazilDepartamento de Clínica e Cirurgia Veterinárias, Universidade Federal de Minas Gerais—UFMG, Belo Horizonte 31270-901, MG, BrazilLaboratório de Arbovírus e Vírus Hemorrágicos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Arbovírus e Vírus Hemorrágicos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilDepartamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal Fluminense—UFF, Niterói 24210-346, RJ, BrazilLaboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilLaboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz—Fiocruz, Rio de Janeiro 21040-900, RJ, BrazilHepatitis E virus (HEV) infection has been demonstrated in various animal species; those recognized as potential zoonotic reservoirs pose a considerable risk to public health. In Brazil, HEV-3 is the only genotype identified in humans and swine nationwide, in a colony-breeding cynomolgus monkey and, recently, in bovines and capybara. There is no information regarding HEV exposure in the equine population in Brazil. This study aimed to investigate anti-HEV antibodies and viral RNA in serum samples from horses slaughtered for meat export and those bred for sport/reproduction purposes. We used a commercially available ELISA kit modified to detect species-specific anti-HEV, using an anti-horse IgG-peroxidase conjugate and evaluating different cutoff formulas and assay precision. Serum samples (n = 257) were tested for anti-HEV IgG and HEV RNA by nested RT-PCR and RT-qPCR. The overall anti-HEV seroprevalence was 26.5% (68/257) without the detection of HEV RNA. Most municipalities (53.3%) and farms (58.8%) had positive horses. Animals slaughtered for human consumption had higher risk of HEV exposure (45.5%) than those bred for sports or reproduction (6.4%) (<i>p</i> < 0.0001). The statistical analysis revealed sex and breeding system as possible risk-associated factors. The first serological evidence of HEV circulation in Brazilian equines reinforces the need for the surveillance of HEV host expansion in a one-health approach.https://www.mdpi.com/2076-2607/11/11/2743hepatitis E virushorsesequine anti-HEV IgGzoonosis |
| spellingShingle | Caroline Roberta Soares Salgado Aldaleia do Nascimento e Silva Igor Falco Arruda Patrícia Riddell Millar Maria Regina Reis Amendoeira Luciane Almeida Amado Leon Raffaella Bertoni Cavalcanti Teixeira Jorge Tiburcio Barbosa de Lima Flávia Löwen Levy Chalhoub Ana Maria Bispo de Filippis Ana Beatriz Monteiro Fonseca Jaqueline Mendes de Oliveira Marcelo Alves Pinto Andreza Soriano Figueiredo Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines Microorganisms hepatitis E virus horses equine anti-HEV IgG zoonosis |
| title | Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines |
| title_full | Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines |
| title_fullStr | Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines |
| title_full_unstemmed | Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines |
| title_short | Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines |
| title_sort | serological evidence of hepatitis e virus infection in brazilian equines |
| topic | hepatitis E virus horses equine anti-HEV IgG zoonosis |
| url | https://www.mdpi.com/2076-2607/11/11/2743 |
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