A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters

The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated a...

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Main Authors: Andrea Martha Freire-Peñaherrera, Ana Tirapé, Jerry Landívar-Zambrano, Juan Manuel Cevallos-Cevallos
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Marine Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full
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author Andrea Martha Freire-Peñaherrera
Ana Tirapé
Jerry Landívar-Zambrano
Juan Manuel Cevallos-Cevallos
Juan Manuel Cevallos-Cevallos
author_facet Andrea Martha Freire-Peñaherrera
Ana Tirapé
Jerry Landívar-Zambrano
Juan Manuel Cevallos-Cevallos
Juan Manuel Cevallos-Cevallos
author_sort Andrea Martha Freire-Peñaherrera
collection DOAJ
description The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated against V. vulnificus. The sensitivity of V. vulnificus to A5 was first assessed in vitro using selected solid media as well as autoclaved oysters (Crassostrea gigas). Then, the ability of A5 to colonize live oysters was evaluated, and the biocontrol efficacy was investigated in vivo using oysters inoculated with V. vulnificus before or after inoculation with A5. The survival of the pathogen in oysters was evaluated after 2, 3, 4, and 6 days of exposure to A5 in all the experiments. In vitro, A5 showed inhibition halos of 18 mm against V. vulnificus. In autoclaved oysters, A5 caused a significant reduction in the levels of V. vulnificus on day 2 at 5.14 log CFU/g, but the pathogen’s counts were restored after day 3. In vivo, A5 was able to survive in live oysters and prevented the colonization of V. vulnificus only when the biocontrol agent was inoculated before the pathogen. Results show the potential of A5 to prevent V. vulnificus uptake by oysters when administered prior to the pathogen.
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spelling doaj.art-2f18c7172ea04e5c8738c28e2e06713b2022-12-21T22:57:08ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-11-01710.3389/fmars.2020.596343596343A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas OystersAndrea Martha Freire-Peñaherrera0Ana Tirapé1Jerry Landívar-Zambrano2Juan Manuel Cevallos-Cevallos3Juan Manuel Cevallos-Cevallos4Faculty of Maritime Engineering and Marine Sciences, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorFaculty of Life Sciences, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorFaculty of Maritime Engineering and Marine Sciences, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorFaculty of Life Sciences, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorBiotechnology Research Center, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorThe pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated against V. vulnificus. The sensitivity of V. vulnificus to A5 was first assessed in vitro using selected solid media as well as autoclaved oysters (Crassostrea gigas). Then, the ability of A5 to colonize live oysters was evaluated, and the biocontrol efficacy was investigated in vivo using oysters inoculated with V. vulnificus before or after inoculation with A5. The survival of the pathogen in oysters was evaluated after 2, 3, 4, and 6 days of exposure to A5 in all the experiments. In vitro, A5 showed inhibition halos of 18 mm against V. vulnificus. In autoclaved oysters, A5 caused a significant reduction in the levels of V. vulnificus on day 2 at 5.14 log CFU/g, but the pathogen’s counts were restored after day 3. In vivo, A5 was able to survive in live oysters and prevented the colonization of V. vulnificus only when the biocontrol agent was inoculated before the pathogen. Results show the potential of A5 to prevent V. vulnificus uptake by oysters when administered prior to the pathogen.https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/fullfoodborne diseasevibriosisshellfishbiocontrolraw food
spellingShingle Andrea Martha Freire-Peñaherrera
Ana Tirapé
Jerry Landívar-Zambrano
Juan Manuel Cevallos-Cevallos
Juan Manuel Cevallos-Cevallos
A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
Frontiers in Marine Science
foodborne disease
vibriosis
shellfish
biocontrol
raw food
title A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
title_full A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
title_fullStr A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
title_full_unstemmed A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
title_short A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
title_sort strain of bacillus amyloliquefaciens can prevent vibrio vulnificus colonization in crassostrea gigas oysters
topic foodborne disease
vibriosis
shellfish
biocontrol
raw food
url https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full
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