A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters
The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated a...
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Frontiers Media S.A.
2020-11-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full |
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author | Andrea Martha Freire-Peñaherrera Ana Tirapé Jerry Landívar-Zambrano Juan Manuel Cevallos-Cevallos Juan Manuel Cevallos-Cevallos |
author_facet | Andrea Martha Freire-Peñaherrera Ana Tirapé Jerry Landívar-Zambrano Juan Manuel Cevallos-Cevallos Juan Manuel Cevallos-Cevallos |
author_sort | Andrea Martha Freire-Peñaherrera |
collection | DOAJ |
description | The pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated against V. vulnificus. The sensitivity of V. vulnificus to A5 was first assessed in vitro using selected solid media as well as autoclaved oysters (Crassostrea gigas). Then, the ability of A5 to colonize live oysters was evaluated, and the biocontrol efficacy was investigated in vivo using oysters inoculated with V. vulnificus before or after inoculation with A5. The survival of the pathogen in oysters was evaluated after 2, 3, 4, and 6 days of exposure to A5 in all the experiments. In vitro, A5 showed inhibition halos of 18 mm against V. vulnificus. In autoclaved oysters, A5 caused a significant reduction in the levels of V. vulnificus on day 2 at 5.14 log CFU/g, but the pathogen’s counts were restored after day 3. In vivo, A5 was able to survive in live oysters and prevented the colonization of V. vulnificus only when the biocontrol agent was inoculated before the pathogen. Results show the potential of A5 to prevent V. vulnificus uptake by oysters when administered prior to the pathogen. |
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series | Frontiers in Marine Science |
spelling | doaj.art-2f18c7172ea04e5c8738c28e2e06713b2022-12-21T22:57:08ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-11-01710.3389/fmars.2020.596343596343A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas OystersAndrea Martha Freire-Peñaherrera0Ana Tirapé1Jerry Landívar-Zambrano2Juan Manuel Cevallos-Cevallos3Juan Manuel Cevallos-Cevallos4Faculty of Maritime Engineering and Marine Sciences, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorFaculty of Life Sciences, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorFaculty of Maritime Engineering and Marine Sciences, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorFaculty of Life Sciences, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorBiotechnology Research Center, Escuela Superior Politécnica del Litoral, ESPOL Polytechnic University, Guayaquil, EcuadorThe pathogen Vibrio vulnificus has been associated with the majority of clinical cases of septicemia and deaths attributed to shellfish consumption. However, reports on biocontrol agents against this pathogen are scarce. In this study, the strain A5 of Bacillus amyloliquefaciens (A5) was evaluated against V. vulnificus. The sensitivity of V. vulnificus to A5 was first assessed in vitro using selected solid media as well as autoclaved oysters (Crassostrea gigas). Then, the ability of A5 to colonize live oysters was evaluated, and the biocontrol efficacy was investigated in vivo using oysters inoculated with V. vulnificus before or after inoculation with A5. The survival of the pathogen in oysters was evaluated after 2, 3, 4, and 6 days of exposure to A5 in all the experiments. In vitro, A5 showed inhibition halos of 18 mm against V. vulnificus. In autoclaved oysters, A5 caused a significant reduction in the levels of V. vulnificus on day 2 at 5.14 log CFU/g, but the pathogen’s counts were restored after day 3. In vivo, A5 was able to survive in live oysters and prevented the colonization of V. vulnificus only when the biocontrol agent was inoculated before the pathogen. Results show the potential of A5 to prevent V. vulnificus uptake by oysters when administered prior to the pathogen.https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/fullfoodborne diseasevibriosisshellfishbiocontrolraw food |
spellingShingle | Andrea Martha Freire-Peñaherrera Ana Tirapé Jerry Landívar-Zambrano Juan Manuel Cevallos-Cevallos Juan Manuel Cevallos-Cevallos A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters Frontiers in Marine Science foodborne disease vibriosis shellfish biocontrol raw food |
title | A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_full | A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_fullStr | A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_full_unstemmed | A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_short | A Strain of Bacillus amyloliquefaciens Can Prevent Vibrio vulnificus Colonization in Crassostrea gigas Oysters |
title_sort | strain of bacillus amyloliquefaciens can prevent vibrio vulnificus colonization in crassostrea gigas oysters |
topic | foodborne disease vibriosis shellfish biocontrol raw food |
url | https://www.frontiersin.org/articles/10.3389/fmars.2020.596343/full |
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