Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells
Summary: Air-liquid organotypic culture models enable the study of the cellular crosstalk in the tumor microenvironment. This 3D assay recapitulates the tumor niche more faithfully than 2D culture systems and represents a versatile platform that can be easily adapted to different types of cancer cel...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722005159 |
| _version_ | 1818484158381424640 |
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| author | Tania Cid-Diaz Andrea C. Lodeiro Angeles Duran Jorge Moscat Maria T. Diaz-Meco |
| author_facet | Tania Cid-Diaz Andrea C. Lodeiro Angeles Duran Jorge Moscat Maria T. Diaz-Meco |
| author_sort | Tania Cid-Diaz |
| collection | DOAJ |
| description | Summary: Air-liquid organotypic culture models enable the study of the cellular crosstalk in the tumor microenvironment. This 3D assay recapitulates the tumor niche more faithfully than 2D culture systems and represents a versatile platform that can be easily adapted to different types of cancer cells, stromal components, or ECM composition. Here, we detail the steps to build an organotypic culture including the preparation of the organotypic structure, organotypic gels, cell seeding, gel casting, membrane processing, and image and data analysis.For complete details on the use and execution of this protocol, please refer to Linares et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-12-10T15:51:25Z |
| format | Article |
| id | doaj.art-2f1b844e394e4772baeb0915e5d617a5 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-10T15:51:25Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-2f1b844e394e4772baeb0915e5d617a52022-12-22T01:42:48ZengElsevierSTAR Protocols2666-16672022-09-0133101635Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cellsTania Cid-Diaz0Andrea C. Lodeiro1Angeles Duran2Jorge Moscat3Maria T. Diaz-Meco4Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA; Corresponding authorDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USADepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USADepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USADepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA; Corresponding authorSummary: Air-liquid organotypic culture models enable the study of the cellular crosstalk in the tumor microenvironment. This 3D assay recapitulates the tumor niche more faithfully than 2D culture systems and represents a versatile platform that can be easily adapted to different types of cancer cells, stromal components, or ECM composition. Here, we detail the steps to build an organotypic culture including the preparation of the organotypic structure, organotypic gels, cell seeding, gel casting, membrane processing, and image and data analysis.For complete details on the use and execution of this protocol, please refer to Linares et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722005159Cell BiologyCell cultureCancerTissue EngineeringBiotechnology and bioengineering |
| spellingShingle | Tania Cid-Diaz Andrea C. Lodeiro Angeles Duran Jorge Moscat Maria T. Diaz-Meco Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells STAR Protocols Cell Biology Cell culture Cancer Tissue Engineering Biotechnology and bioengineering |
| title | Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells |
| title_full | Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells |
| title_fullStr | Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells |
| title_full_unstemmed | Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells |
| title_short | Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells |
| title_sort | air liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells |
| topic | Cell Biology Cell culture Cancer Tissue Engineering Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722005159 |
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