Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage Industry
This study examines the in vitro effect of Quillaja saponaria extracts on Asaia spp. planktonic cells and biofilms, in comparison and combination with two disinfectants: peracetic acid and N-ethyl-N,N-dimethylhexadecylammonium bromide. The growth of six bacterial strains was evaluated spectrophotome...
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MDPI AG
2018-03-01
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| Series: | Applied Sciences |
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| Online Access: | http://www.mdpi.com/2076-3417/8/3/368 |
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| author | Hubert Antolak Urszula Mizerska Joanna Berłowska Anna Otlewska Dorota Kręgiel |
| author_facet | Hubert Antolak Urszula Mizerska Joanna Berłowska Anna Otlewska Dorota Kręgiel |
| author_sort | Hubert Antolak |
| collection | DOAJ |
| description | This study examines the in vitro effect of Quillaja saponaria extracts on Asaia spp. planktonic cells and biofilms, in comparison and combination with two disinfectants: peracetic acid and N-ethyl-N,N-dimethylhexadecylammonium bromide. The growth of six bacterial strains was evaluated spectrophotometrically. Biofilm eradication was determined using the plate count method and luminometry. The planktonic cells were characterized by relatively high resistance to peracetic acid and higher sensitivity to N-ethylo-N,N-dimethylohexadecylioamonium bromide. In almost all the tested strains, growth was inhibited by 0.125% (v/v) peracetic acid and 0.0313% (w/v) quaternary ammonium compound. However, combinations of cell pretreatment using saponin and peracetic acid action were the most efficient against both planktonic and biofilm cells. The minimum inhibitory concentrations for peracetic acid were 4–8 times lower than those for bacterial strains without preliminary saponin action. Eradication of Asaia spp. biofilms reduced the number of living cells by 4–5 logarithmic units. These results demonstrate the synergetic action of saponin extract and disinfectant, and could be useful in the development of industrial strategies against Asaia spp. biofilms. |
| first_indexed | 2024-12-11T02:17:01Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-11T02:17:01Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-2f1bcb0014ba418ba353f2883ae6fd0c2022-12-22T01:24:10ZengMDPI AGApplied Sciences2076-34172018-03-018336810.3390/app8030368app8030368Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage IndustryHubert Antolak0Urszula Mizerska1Joanna Berłowska2Anna Otlewska3Dorota Kręgiel4Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Science, Lodz University of Technology, 171/173 Wolczanska, 90-924 Lodz, PolandCenter of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, PolandInstitute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Science, Lodz University of Technology, 171/173 Wolczanska, 90-924 Lodz, PolandInstitute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Science, Lodz University of Technology, 171/173 Wolczanska, 90-924 Lodz, PolandInstitute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Science, Lodz University of Technology, 171/173 Wolczanska, 90-924 Lodz, PolandThis study examines the in vitro effect of Quillaja saponaria extracts on Asaia spp. planktonic cells and biofilms, in comparison and combination with two disinfectants: peracetic acid and N-ethyl-N,N-dimethylhexadecylammonium bromide. The growth of six bacterial strains was evaluated spectrophotometrically. Biofilm eradication was determined using the plate count method and luminometry. The planktonic cells were characterized by relatively high resistance to peracetic acid and higher sensitivity to N-ethylo-N,N-dimethylohexadecylioamonium bromide. In almost all the tested strains, growth was inhibited by 0.125% (v/v) peracetic acid and 0.0313% (w/v) quaternary ammonium compound. However, combinations of cell pretreatment using saponin and peracetic acid action were the most efficient against both planktonic and biofilm cells. The minimum inhibitory concentrations for peracetic acid were 4–8 times lower than those for bacterial strains without preliminary saponin action. Eradication of Asaia spp. biofilms reduced the number of living cells by 4–5 logarithmic units. These results demonstrate the synergetic action of saponin extract and disinfectant, and could be useful in the development of industrial strategies against Asaia spp. biofilms.http://www.mdpi.com/2076-3417/8/3/368Quillaja saponinsAsaia spp.planktonic cellsbiofilmsdisinfection |
| spellingShingle | Hubert Antolak Urszula Mizerska Joanna Berłowska Anna Otlewska Dorota Kręgiel Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage Industry Applied Sciences Quillaja saponins Asaia spp. planktonic cells biofilms disinfection |
| title | Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage Industry |
| title_full | Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage Industry |
| title_fullStr | Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage Industry |
| title_full_unstemmed | Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage Industry |
| title_short | Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage Industry |
| title_sort | quillaja saponaria saponins with potential to enhance the effectiveness of disinfection processes in the beverage industry |
| topic | Quillaja saponins Asaia spp. planktonic cells biofilms disinfection |
| url | http://www.mdpi.com/2076-3417/8/3/368 |
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