Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid
Experiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as...
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| Format: | Article |
| Language: | English |
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AOSIS
2012-02-01
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| Series: | Onderstepoort Journal of Veterinary Research |
| Subjects: | |
| Online Access: | http://www.ojvr.org/index.php/ojvr/article/view/413 |
| _version_ | 1818692496760242176 |
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| author | Folashade Olaifa Joseph O. Ayo Suleiman F. Ambali Peter I. Rekwot |
| author_facet | Folashade Olaifa Joseph O. Ayo Suleiman F. Ambali Peter I. Rekwot |
| author_sort | Folashade Olaifa |
| collection | DOAJ |
| description | Experiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (<em>P</em> < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (<em>P</em> > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration. |
| first_indexed | 2024-12-17T12:58:43Z |
| format | Article |
| id | doaj.art-2f1bcc09c89a4901ae8125c2ac27bafd |
| institution | Directory Open Access Journal |
| issn | 0030-2465 2219-0635 |
| language | English |
| last_indexed | 2024-12-17T12:58:43Z |
| publishDate | 2012-02-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Onderstepoort Journal of Veterinary Research |
| spelling | doaj.art-2f1bcc09c89a4901ae8125c2ac27bafd2022-12-21T21:47:25ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352012-02-01791e1e5383Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acidFolashade Olaifa0Joseph O. Ayo1Suleiman F. Ambali2Peter I. Rekwot3Department of Physiology and Pharmacology, Ahmadu Bello UniversityDepartment of Physiology and Pharmacology, Ahmadu Bello UniversityDepartment of Physiology and Pharmacology, Ahmadu Bello UniversityNational Animal Production Research Institute, Ahmadu Bello UniversityExperiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (<em>P</em> < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (<em>P</em> > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration.http://www.ojvr.org/index.php/ojvr/article/view/413Ascorbic aciddonkeyhaemolysismalondialdehydework stress |
| spellingShingle | Folashade Olaifa Joseph O. Ayo Suleiman F. Ambali Peter I. Rekwot Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid Onderstepoort Journal of Veterinary Research Ascorbic acid donkey haemolysis malondialdehyde work stress |
| title | Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid |
| title_full | Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid |
| title_fullStr | Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid |
| title_full_unstemmed | Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid |
| title_short | Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid |
| title_sort | effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid |
| topic | Ascorbic acid donkey haemolysis malondialdehyde work stress |
| url | http://www.ojvr.org/index.php/ojvr/article/view/413 |
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