Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor
Bis(styryl) dye <b>1</b> bearing <i>N</i>-phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe <b>1</b> selectively responds to the presence of Hg<...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/1424-8220/21/2/470 |
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| author | Pavel A. Panchenko Anastasija V. Efremenko Alexey V. Feofanov Mariya A. Ustimova Yuri V. Fedorov Olga A. Fedorova |
| author_facet | Pavel A. Panchenko Anastasija V. Efremenko Alexey V. Feofanov Mariya A. Ustimova Yuri V. Fedorov Olga A. Fedorova |
| author_sort | Pavel A. Panchenko |
| collection | DOAJ |
| description | Bis(styryl) dye <b>1</b> bearing <i>N</i>-phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe <b>1</b> selectively responds to the presence of Hg<sup>2+</sup> via the changes in the emission intensity as well as in the emission band shape, which is a result of formation of the complex with 1:1 metal to ligand ratio (dissociation constant 0.56 ± 0.15 µM). The sensing mechanism is based on the interplay between the RET (resonance energy transfer) and ICT (intramolecular charge transfer) interactions occurring upon the UV/Vis (380 or 405 nm) photoexcitation of both styryl chromophores in probe 1. Bio-imaging studies revealed that the yellow (500–600 nm) to red (600–730 nm) fluorescence intensity ratio decreased from 4.4 ± 0.2 to 1.43 ± 0.10 when cells were exposed to increasing concentration of mercury (II) ions enabling ratiometric quantification of intracellular Hg<sup>2+</sup> concentration in the 37 nM–1 μM range. |
| first_indexed | 2024-03-09T05:10:48Z |
| format | Article |
| id | doaj.art-2f1cb47d989b4e2b8f7221c5c2cb6f42 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T05:10:48Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-2f1cb47d989b4e2b8f7221c5c2cb6f422023-12-03T12:49:05ZengMDPI AGSensors1424-82202021-01-0121247010.3390/s21020470Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether ReceptorPavel A. Panchenko0Anastasija V. Efremenko1Alexey V. Feofanov2Mariya A. Ustimova3Yuri V. Fedorov4Olga A. Fedorova5Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, RussiaBiological Faculty, Lomonosov Moscow State University, 119992 Moscow, RussiaBiological Faculty, Lomonosov Moscow State University, 119992 Moscow, RussiaLaboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, RussiaLaboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, RussiaLaboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, RussiaBis(styryl) dye <b>1</b> bearing <i>N</i>-phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe <b>1</b> selectively responds to the presence of Hg<sup>2+</sup> via the changes in the emission intensity as well as in the emission band shape, which is a result of formation of the complex with 1:1 metal to ligand ratio (dissociation constant 0.56 ± 0.15 µM). The sensing mechanism is based on the interplay between the RET (resonance energy transfer) and ICT (intramolecular charge transfer) interactions occurring upon the UV/Vis (380 or 405 nm) photoexcitation of both styryl chromophores in probe 1. Bio-imaging studies revealed that the yellow (500–600 nm) to red (600–730 nm) fluorescence intensity ratio decreased from 4.4 ± 0.2 to 1.43 ± 0.10 when cells were exposed to increasing concentration of mercury (II) ions enabling ratiometric quantification of intracellular Hg<sup>2+</sup> concentration in the 37 nM–1 μM range.https://www.mdpi.com/1424-8220/21/2/470Hg<sup>2+</sup>ratiometric sensorfluorescence imagingresonance energy transferintramolecular charge transferstyryl dye |
| spellingShingle | Pavel A. Panchenko Anastasija V. Efremenko Alexey V. Feofanov Mariya A. Ustimova Yuri V. Fedorov Olga A. Fedorova Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor Sensors Hg<sup>2+</sup> ratiometric sensor fluorescence imaging resonance energy transfer intramolecular charge transfer styryl dye |
| title | Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor |
| title_full | Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor |
| title_fullStr | Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor |
| title_full_unstemmed | Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor |
| title_short | Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor |
| title_sort | ratiometric detection of mercury ii ions in living cells using fluorescent probe based on bis styryl dye and azadithia 15 crown 5 ether receptor |
| topic | Hg<sup>2+</sup> ratiometric sensor fluorescence imaging resonance energy transfer intramolecular charge transfer styryl dye |
| url | https://www.mdpi.com/1424-8220/21/2/470 |
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