An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis
Abdominal angiostrongyliasis is a potentially fatal zoonotic disease with a broad geographical distribution throughout Central and South America. This study assessed the performance of Angiostrongylus costaricensis eggs as the antigen in an indirect immunofluorescence assay for the determination of...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400002&lng=en&tlng=en |
| _version_ | 1797718344749547520 |
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| author | Elizabeth Abrahams-Sandi Paolo Mesén-Ramírez Dimelsa Suarez-Chacón Katherine Fernández-Quesada |
| author_facet | Elizabeth Abrahams-Sandi Paolo Mesén-Ramírez Dimelsa Suarez-Chacón Katherine Fernández-Quesada |
| author_sort | Elizabeth Abrahams-Sandi |
| collection | DOAJ |
| description | Abdominal angiostrongyliasis is a potentially fatal zoonotic disease with a broad geographical distribution throughout Central and South America. This study assessed the performance of Angiostrongylus costaricensis eggs as the antigen in an indirect immunofluorescence assay for the determination of parasite-specific IgG and IgG1 antibodies. For prevalence studies, an IgG antibody titre > 16 was identified as the diagnostic threshold with the best performance, providing 93.7% sensitivity and 84.6% specificity. Cross reactivity was evaluated with 65 additional samples from patients with other known parasitic infections. Cross reactivity was observed only in samples from individuals infected with Strongyloides stercoralis. For clinical diagnosis, we recommend the determination of IgG only as a screening test. IgG1 determination may be used to increase the specificity of the results for patients with a positive screening test. |
| first_indexed | 2024-03-12T08:49:40Z |
| format | Article |
| id | doaj.art-2f1ef84df6ec4ec9a4faa5b123aabdbf |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:49:40Z |
| publishDate | 2011-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-2f1ef84df6ec4ec9a4faa5b123aabdbf2023-09-02T16:21:38ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-06-01106439039310.1590/S0074-02762011000400002S0074-02762011000400002An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasisElizabeth Abrahams-Sandi0Paolo Mesén-Ramírez1Dimelsa Suarez-Chacón2Katherine Fernández-Quesada3Universidad de Costa RicaInstituto Costarricense de Investigación y Enseñanza en Nutrición y SaludUniversidad de Costa RicaBoston Scientific Costa RicaAbdominal angiostrongyliasis is a potentially fatal zoonotic disease with a broad geographical distribution throughout Central and South America. This study assessed the performance of Angiostrongylus costaricensis eggs as the antigen in an indirect immunofluorescence assay for the determination of parasite-specific IgG and IgG1 antibodies. For prevalence studies, an IgG antibody titre > 16 was identified as the diagnostic threshold with the best performance, providing 93.7% sensitivity and 84.6% specificity. Cross reactivity was evaluated with 65 additional samples from patients with other known parasitic infections. Cross reactivity was observed only in samples from individuals infected with Strongyloides stercoralis. For clinical diagnosis, we recommend the determination of IgG only as a screening test. IgG1 determination may be used to increase the specificity of the results for patients with a positive screening test.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400002&lng=en&tlng=enabdominal angiostrongyliasisimmunodiagnosisimmunofluorescenceegg antigen |
| spellingShingle | Elizabeth Abrahams-Sandi Paolo Mesén-Ramírez Dimelsa Suarez-Chacón Katherine Fernández-Quesada An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis Memorias do Instituto Oswaldo Cruz abdominal angiostrongyliasis immunodiagnosis immunofluorescence egg antigen |
| title | An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis |
| title_full | An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis |
| title_fullStr | An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis |
| title_full_unstemmed | An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis |
| title_short | An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis |
| title_sort | indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis |
| topic | abdominal angiostrongyliasis immunodiagnosis immunofluorescence egg antigen |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400002&lng=en&tlng=en |
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