Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes
Abstract The enteric nervous system (ENS) is a large and complex part of the peripheral nervous system, and it is vital for gut homeostasis. To study the ENS, different hyper- and hypo-innervated model systems have been developed. The NSE-Noggin mouse model was described as one of the few models wit...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-02-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-54337-w |
| _version_ | 1797275059352502272 |
|---|---|
| author | Simone L. Schonkeren Meike S. Thijssen Musa Idris Kim Wouters Joëlle de Vaan Andreas Teubner Marion J. Gijbels Werend Boesmans Veerle Melotte |
| author_facet | Simone L. Schonkeren Meike S. Thijssen Musa Idris Kim Wouters Joëlle de Vaan Andreas Teubner Marion J. Gijbels Werend Boesmans Veerle Melotte |
| author_sort | Simone L. Schonkeren |
| collection | DOAJ |
| description | Abstract The enteric nervous system (ENS) is a large and complex part of the peripheral nervous system, and it is vital for gut homeostasis. To study the ENS, different hyper- and hypo-innervated model systems have been developed. The NSE-Noggin mouse model was described as one of the few models with a higher enteric neuronal density in the colon. However, in our hands NSE-Noggin mice did not present with a hyperganglionic phenotype. NSE-Noggin mice were phenotyped based on fur appearance, genotyped and DNA sequenced to demonstrate transgene and intact NSE-Noggin-IRES-EGFP construct presence, and RNA expression of Noggin was shown to be upregulated. Positive EGFP staining in the plexus of NSE-Noggin mice also confirmed Noggin protein expression. Myenteric plexus preparations of the colon were examined to quantify both the overall density of enteric neurons and the proportions of enteric neurons expressing specific subtype markers. The total number of enteric neurons in the colonic myenteric plexus of transgenic mice did not differ significantly from wild types, nor did the proportion of calbindin, calretinin, or serotonin immunoreactive myenteric neurons. Possible reasons as to why the hyperinnervated phenotype could not be observed in contrast with original studies using this mouse model are discussed, including study design, influence of microbiota, and other environmental variables. |
| first_indexed | 2024-03-07T15:08:00Z |
| format | Article |
| id | doaj.art-2f1f4a5c64f7470e8697c082d203f6cf |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:08:00Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2f1f4a5c64f7470e8697c082d203f6cf2024-03-05T18:48:32ZengNature PortfolioScientific Reports2045-23222024-02-0114111010.1038/s41598-024-54337-wDifferences in enteric neuronal density in the NSE-Noggin mouse model across institutesSimone L. Schonkeren0Meike S. Thijssen1Musa Idris2Kim Wouters3Joëlle de Vaan4Andreas Teubner5Marion J. Gijbels6Werend Boesmans7Veerle Melotte8Department of Pathology, GROW–Research Institute for Oncology and Reproduction, Maastricht University Medical CenterDepartment of Pathology, GROW–Research Institute for Oncology and Reproduction, Maastricht University Medical CenterDepartment of Pathology, GROW–Research Institute for Oncology and Reproduction, Maastricht University Medical CenterDepartment of Pathology, GROW–Research Institute for Oncology and Reproduction, Maastricht University Medical CenterDepartment of Pathology, GROW–Research Institute for Oncology and Reproduction, Maastricht University Medical CenterCentral Animal Facility, Faculty of Health, Medicine & Life Sciences, Maastricht UniversityDepartment of Pathology, GROW–Research Institute for Oncology and Reproduction, Maastricht University Medical CenterDepartment of Pathology, GROW–Research Institute for Oncology and Reproduction, Maastricht University Medical CenterDepartment of Pathology, GROW–Research Institute for Oncology and Reproduction, Maastricht University Medical CenterAbstract The enteric nervous system (ENS) is a large and complex part of the peripheral nervous system, and it is vital for gut homeostasis. To study the ENS, different hyper- and hypo-innervated model systems have been developed. The NSE-Noggin mouse model was described as one of the few models with a higher enteric neuronal density in the colon. However, in our hands NSE-Noggin mice did not present with a hyperganglionic phenotype. NSE-Noggin mice were phenotyped based on fur appearance, genotyped and DNA sequenced to demonstrate transgene and intact NSE-Noggin-IRES-EGFP construct presence, and RNA expression of Noggin was shown to be upregulated. Positive EGFP staining in the plexus of NSE-Noggin mice also confirmed Noggin protein expression. Myenteric plexus preparations of the colon were examined to quantify both the overall density of enteric neurons and the proportions of enteric neurons expressing specific subtype markers. The total number of enteric neurons in the colonic myenteric plexus of transgenic mice did not differ significantly from wild types, nor did the proportion of calbindin, calretinin, or serotonin immunoreactive myenteric neurons. Possible reasons as to why the hyperinnervated phenotype could not be observed in contrast with original studies using this mouse model are discussed, including study design, influence of microbiota, and other environmental variables.https://doi.org/10.1038/s41598-024-54337-w |
| spellingShingle | Simone L. Schonkeren Meike S. Thijssen Musa Idris Kim Wouters Joëlle de Vaan Andreas Teubner Marion J. Gijbels Werend Boesmans Veerle Melotte Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes Scientific Reports |
| title | Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes |
| title_full | Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes |
| title_fullStr | Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes |
| title_full_unstemmed | Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes |
| title_short | Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes |
| title_sort | differences in enteric neuronal density in the nse noggin mouse model across institutes |
| url | https://doi.org/10.1038/s41598-024-54337-w |
| work_keys_str_mv | AT simonelschonkeren differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes AT meikesthijssen differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes AT musaidris differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes AT kimwouters differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes AT joelledevaan differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes AT andreasteubner differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes AT marionjgijbels differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes AT werendboesmans differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes AT veerlemelotte differencesinentericneuronaldensityinthensenogginmousemodelacrossinstitutes |