Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens

Background and Objectives: Silver nanoparticles (AgNPs) have been found to have multiple uses as antibacterial, antifungal and anti-biofilm agents because of their biological activities and safety. The present study was aimed to analyze the antimicrobial and anti-biofilm activities as well as the cy...

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Main Authors: Asmaa R. Ali, Haneya A.A. Anani, Fatma M. Selim
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2021-12-01
Series:Iranian Journal of Microbiology
Subjects:
Online Access:https://ijm.tums.ac.ir/index.php/ijm/article/view/3303
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author Asmaa R. Ali
Haneya A.A. Anani
Fatma M. Selim
author_facet Asmaa R. Ali
Haneya A.A. Anani
Fatma M. Selim
author_sort Asmaa R. Ali
collection DOAJ
description Background and Objectives: Silver nanoparticles (AgNPs) have been found to have multiple uses as antibacterial, antifungal and anti-biofilm agents because of their biological activities and safety. The present study was aimed to analyze the antimicrobial and anti-biofilm activities as well as the cytotoxic effect of AgNPs against different human pathogens. Materials and Methods: AgNPs were synthesized using cell free supernatants of Escherichia coli (ATCC 25922), Enterococcus faecalis (ATCC 19433), Pseudomonas aeruginosa (ATCC 27856), Enterobacter cloacae (ATCC 13047) and Penicillium oxalicum strain, then were analyzed using UV/Vis Spectral Analysis, Transmission electron microscopy (TEM). Scanning Electron Microscope (SEM) and Energy Dispersive-X-ray Spectroscopy (EDX) analysis. Antimicrobial activities of biosynthesized AgNPs were assessed with selected antimicrobial agents against multidrug resistant bacteria and candida. Anti-biofilm and cytotoxicity assays of these biosynthesized AgNPs were also done. Results: The synthesis of AgNPs were confirmed through observed color change and monitoring UV-Vis spectrum which showed homogeneous (little agglomeration) distribution of silver nanoparticles. TEM and SEM have shown that the particle size ranged from 13 to 34 (nm) with spherical shape and a high signal with EDX analysis. Antibacterial and antifungal efficacy of antibiotics and fluconazole were increased in combination with biosynthesized AgNPs against resistant bacteria and candida. Significant reduction in biofilm formation was found better with Penicillium oxalicum AgNPs against biofilm forming bacteria. Conclusion: Penicillium oxalicum has the best effect towards synthesizing AgNPs, for antimicrobial activities against resistant bacteria and candida, in addition to anti-biofilm activities against biofilm forming Staphylococcus aureus and E. coli and the safest cytotoxicity effect on (MRC-5) cell line.
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spelling doaj.art-2f24f0950e594a808a3a417f1a17b2e12022-12-21T17:45:04ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472021-12-01136Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogensAsmaa R. Ali0Haneya A.A. Anani1Fatma M. Selim2Department of Medical Microbiology and Immunology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, EgyptBackground and Objectives: Silver nanoparticles (AgNPs) have been found to have multiple uses as antibacterial, antifungal and anti-biofilm agents because of their biological activities and safety. The present study was aimed to analyze the antimicrobial and anti-biofilm activities as well as the cytotoxic effect of AgNPs against different human pathogens. Materials and Methods: AgNPs were synthesized using cell free supernatants of Escherichia coli (ATCC 25922), Enterococcus faecalis (ATCC 19433), Pseudomonas aeruginosa (ATCC 27856), Enterobacter cloacae (ATCC 13047) and Penicillium oxalicum strain, then were analyzed using UV/Vis Spectral Analysis, Transmission electron microscopy (TEM). Scanning Electron Microscope (SEM) and Energy Dispersive-X-ray Spectroscopy (EDX) analysis. Antimicrobial activities of biosynthesized AgNPs were assessed with selected antimicrobial agents against multidrug resistant bacteria and candida. Anti-biofilm and cytotoxicity assays of these biosynthesized AgNPs were also done. Results: The synthesis of AgNPs were confirmed through observed color change and monitoring UV-Vis spectrum which showed homogeneous (little agglomeration) distribution of silver nanoparticles. TEM and SEM have shown that the particle size ranged from 13 to 34 (nm) with spherical shape and a high signal with EDX analysis. Antibacterial and antifungal efficacy of antibiotics and fluconazole were increased in combination with biosynthesized AgNPs against resistant bacteria and candida. Significant reduction in biofilm formation was found better with Penicillium oxalicum AgNPs against biofilm forming bacteria. Conclusion: Penicillium oxalicum has the best effect towards synthesizing AgNPs, for antimicrobial activities against resistant bacteria and candida, in addition to anti-biofilm activities against biofilm forming Staphylococcus aureus and E. coli and the safest cytotoxicity effect on (MRC-5) cell line.https://ijm.tums.ac.ir/index.php/ijm/article/view/3303Penicillium oxalicum;Sliver nanoparticles;Biofilm;Cytotoxicity
spellingShingle Asmaa R. Ali
Haneya A.A. Anani
Fatma M. Selim
Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens
Iranian Journal of Microbiology
Penicillium oxalicum;
Sliver nanoparticles;
Biofilm;
Cytotoxicity
title Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens
title_full Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens
title_fullStr Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens
title_full_unstemmed Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens
title_short Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens
title_sort biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens
topic Penicillium oxalicum;
Sliver nanoparticles;
Biofilm;
Cytotoxicity
url https://ijm.tums.ac.ir/index.php/ijm/article/view/3303
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AT haneyaaaanani biologicallyformedsilvernanoparticlesandinvitrostudyoftheirantimicrobialactivitiesonresistantpathogens
AT fatmamselim biologicallyformedsilvernanoparticlesandinvitrostudyoftheirantimicrobialactivitiesonresistantpathogens