Expression Pattern of MicroRNA-21 during the Liver Ischemia/Reperfusion
Ischemia/reperfusion (I/R) injury in cadaveric liver transplantation is not avoidable. Liver I/R injury is an important phenomenon in hepatic damage. MicroRNA-21 (miR-21) plays an important role in I/R injury. The present study aimed to determine the expression pattern of miR-21 in liver I/R injury/...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2021-02-01
|
| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2828 |
| _version_ | 1818394915066871808 |
|---|---|
| author | Alireza Salah Mohammad Hossein Karimi Javad Sajedianfard Saeed Nazifi Ramin Yaghobi |
| author_facet | Alireza Salah Mohammad Hossein Karimi Javad Sajedianfard Saeed Nazifi Ramin Yaghobi |
| author_sort | Alireza Salah |
| collection | DOAJ |
| description | Ischemia/reperfusion (I/R) injury in cadaveric liver transplantation is not avoidable. Liver I/R injury is an important phenomenon in hepatic damage. MicroRNA-21 (miR-21) plays an important role in I/R injury. The present study aimed to determine the expression pattern of miR-21 in liver I/R injury/recovery and its correlation with the immunologic transmission signals pathways several days post-reperfusion.
In an animal model for I/R in the liver, 40 male Balb/c mice were divided into 3 groups. The animals were monitored for 3 and 24 hours, and also for 4, 7, 14, and 28 days post-reperfusion. Liver tissue damage was assessed by histopathology. The plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total antioxidant capacity (TAC) levels were measured with enzymatic assays. MiR-21, programmed cell death 4 (PDCD4) mRNA, T-cell-restricted intracellular antigen 1 (TIA1) mRNA, and fas ligand (FASL) mRNA expression levels were measured; using reverse transcription-polymerase chain reaction (RT-PCR) at different times after the reperfusion in liver tissue and blood.
Histopathology and plasma ALT, AST, ALP, and TAC levels confirmed liver damage induced by I/R injury. MiR-21 increased by twofold in the liver tissue and on the inflammatory phase after 24 hours of reperfusion; it then continued to decrease up to day 7 post-reperfusion. Afterward, it continued to rise slightly up to day 14 post-reperfusion. This trend was in parallel with the recovery of the liver damage.
MiR-21 expression level in the liver and blood is a predictor of the extent of I/R injury. |
| first_indexed | 2024-12-14T06:08:47Z |
| format | Article |
| id | doaj.art-2f27a33aa8104a90b64a2b8544c28918 |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-14T06:08:47Z |
| publishDate | 2021-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-2f27a33aa8104a90b64a2b8544c289182022-12-21T23:14:13ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492021-02-0120110.18502/ijaai.v20i1.5415Expression Pattern of MicroRNA-21 during the Liver Ischemia/ReperfusionAlireza Salah0Mohammad Hossein Karimi1Javad Sajedianfard2Saeed Nazifi3Ramin Yaghobi4Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranOrgan Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, IranOrgan Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranIschemia/reperfusion (I/R) injury in cadaveric liver transplantation is not avoidable. Liver I/R injury is an important phenomenon in hepatic damage. MicroRNA-21 (miR-21) plays an important role in I/R injury. The present study aimed to determine the expression pattern of miR-21 in liver I/R injury/recovery and its correlation with the immunologic transmission signals pathways several days post-reperfusion. In an animal model for I/R in the liver, 40 male Balb/c mice were divided into 3 groups. The animals were monitored for 3 and 24 hours, and also for 4, 7, 14, and 28 days post-reperfusion. Liver tissue damage was assessed by histopathology. The plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total antioxidant capacity (TAC) levels were measured with enzymatic assays. MiR-21, programmed cell death 4 (PDCD4) mRNA, T-cell-restricted intracellular antigen 1 (TIA1) mRNA, and fas ligand (FASL) mRNA expression levels were measured; using reverse transcription-polymerase chain reaction (RT-PCR) at different times after the reperfusion in liver tissue and blood. Histopathology and plasma ALT, AST, ALP, and TAC levels confirmed liver damage induced by I/R injury. MiR-21 increased by twofold in the liver tissue and on the inflammatory phase after 24 hours of reperfusion; it then continued to decrease up to day 7 post-reperfusion. Afterward, it continued to rise slightly up to day 14 post-reperfusion. This trend was in parallel with the recovery of the liver damage. MiR-21 expression level in the liver and blood is a predictor of the extent of I/R injury.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2828IschemiaLiverMiceMicroRNA-21Reperfusion |
| spellingShingle | Alireza Salah Mohammad Hossein Karimi Javad Sajedianfard Saeed Nazifi Ramin Yaghobi Expression Pattern of MicroRNA-21 during the Liver Ischemia/Reperfusion Iranian Journal of Allergy, Asthma and Immunology Ischemia Liver Mice MicroRNA-21 Reperfusion |
| title | Expression Pattern of MicroRNA-21 during the Liver Ischemia/Reperfusion |
| title_full | Expression Pattern of MicroRNA-21 during the Liver Ischemia/Reperfusion |
| title_fullStr | Expression Pattern of MicroRNA-21 during the Liver Ischemia/Reperfusion |
| title_full_unstemmed | Expression Pattern of MicroRNA-21 during the Liver Ischemia/Reperfusion |
| title_short | Expression Pattern of MicroRNA-21 during the Liver Ischemia/Reperfusion |
| title_sort | expression pattern of microrna 21 during the liver ischemia reperfusion |
| topic | Ischemia Liver Mice MicroRNA-21 Reperfusion |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2828 |
| work_keys_str_mv | AT alirezasalah expressionpatternofmicrorna21duringtheliverischemiareperfusion AT mohammadhosseinkarimi expressionpatternofmicrorna21duringtheliverischemiareperfusion AT javadsajedianfard expressionpatternofmicrorna21duringtheliverischemiareperfusion AT saeednazifi expressionpatternofmicrorna21duringtheliverischemiareperfusion AT raminyaghobi expressionpatternofmicrorna21duringtheliverischemiareperfusion |