Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, Brazil
ABSTRACT Free-range chickens may ingest oocysts of T. gondii present in the environment and consequently harbor virulent strains of this parasite in different tissues, without any clinical signs. Isolation of T. gondii through bioassays on mice and cats from naturally infected chicken tissues has be...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2020-08-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000401339&tlng=en |
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| author | G. Camillo M.E.A. Machado G.C. Cadore P. Bräunig M.C. Venturini L.L. Pardini L.D. Barros J.L. Garcia L.A. Sangioni F.S.F. Vogel |
| author_facet | G. Camillo M.E.A. Machado G.C. Cadore P. Bräunig M.C. Venturini L.L. Pardini L.D. Barros J.L. Garcia L.A. Sangioni F.S.F. Vogel |
| author_sort | G. Camillo |
| collection | DOAJ |
| description | ABSTRACT Free-range chickens may ingest oocysts of T. gondii present in the environment and consequently harbor virulent strains of this parasite in different tissues, without any clinical signs. Isolation of T. gondii through bioassays on mice and cats from naturally infected chicken tissues has been described in several countries, demonstrating the importance of free-range chickens in the transmission of this parasite. The aim of this study was the genotypic characterization of T. gondii isolates obtained from naturally infected free-range chickens in a rural area of the state of Rio Grande do Sul, Brazil. Brain and heart tissue from 12 chickens seropositive for T. gondii were processed using peptic digestion technique for parasite isolation. From 12 samples subjected to mouse bioassay, nine isolates were obtained. RFLP-PCR genotypic characterization was performed using 11 genetic markers: SAG1, 5'-3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. Genetic characterization of the isolates revealed the presence of five atypical genotypes according to ToxoDB (# 11, # 55, # 64, # 140 and # 163). Our results showed a wide genetic diversity of T. gondii in free-range chickens in this region. |
| first_indexed | 2024-12-20T15:30:30Z |
| format | Article |
| id | doaj.art-2f27eab775f244488926e2cc8d83e7c8 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-20T15:30:30Z |
| publishDate | 2020-08-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-2f27eab775f244488926e2cc8d83e7c82022-12-21T19:35:36ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622020-08-017241339134510.1590/1678-4162-11732Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, BrazilG. Camillohttps://orcid.org/0000-0003-0971-706XM.E.A. Machadohttps://orcid.org/0000-0001-8312-1622G.C. Cadorehttps://orcid.org/0000-0002-2253-130XP. Bräunighttps://orcid.org/0000-0001-5684-0744M.C. Venturinihttps://orcid.org/0000-0002-9750-0284L.L. Pardinihttps://orcid.org/0000-0003-3332-5402L.D. Barroshttps://orcid.org/0000-0002-8560-3948J.L. Garciahttps://orcid.org/0000-0003-1826-7582L.A. Sangionihttps://orcid.org/0000-0002-2364-1084F.S.F. Vogelhttps://orcid.org/0000-0003-1351-9912ABSTRACT Free-range chickens may ingest oocysts of T. gondii present in the environment and consequently harbor virulent strains of this parasite in different tissues, without any clinical signs. Isolation of T. gondii through bioassays on mice and cats from naturally infected chicken tissues has been described in several countries, demonstrating the importance of free-range chickens in the transmission of this parasite. The aim of this study was the genotypic characterization of T. gondii isolates obtained from naturally infected free-range chickens in a rural area of the state of Rio Grande do Sul, Brazil. Brain and heart tissue from 12 chickens seropositive for T. gondii were processed using peptic digestion technique for parasite isolation. From 12 samples subjected to mouse bioassay, nine isolates were obtained. RFLP-PCR genotypic characterization was performed using 11 genetic markers: SAG1, 5'-3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. Genetic characterization of the isolates revealed the presence of five atypical genotypes according to ToxoDB (# 11, # 55, # 64, # 140 and # 163). Our results showed a wide genetic diversity of T. gondii in free-range chickens in this region.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000401339&tlng=engenotypingtoxoplasmosismouse bioassayPCR-RFLP |
| spellingShingle | G. Camillo M.E.A. Machado G.C. Cadore P. Bräunig M.C. Venturini L.L. Pardini L.D. Barros J.L. Garcia L.A. Sangioni F.S.F. Vogel Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, Brazil Arquivo Brasileiro de Medicina Veterinária e Zootecnia genotyping toxoplasmosis mouse bioassay PCR-RFLP |
| title | Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, Brazil |
| title_full | Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, Brazil |
| title_fullStr | Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, Brazil |
| title_full_unstemmed | Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, Brazil |
| title_short | Toxoplasma gondii genotyping from free-range chickens (Gallus gallus domesticus) in a rural area of Rio Grande do Sul, Brazil |
| title_sort | toxoplasma gondii genotyping from free range chickens gallus gallus domesticus in a rural area of rio grande do sul brazil |
| topic | genotyping toxoplasmosis mouse bioassay PCR-RFLP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000401339&tlng=en |
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