Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A
Abstract The cholecystokinin (CCK)/gastrin family peptides are involved in regulation of feeding and digestion in vertebrates. In the ascidian Ciona intestinalis type A (Ciona robusta), cionin, a CCK/gastrin family peptide, has been identified. Cionin is expressed exclusively in the central nervous...
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-55908-7 |
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| author | Shiho Taniguchi Satoshi Nakayama Rin Iguchi Yasunori Sasakura Honoo Satake Shuichi Wada Nobuo Suzuki Michio Ogasawara Toshio Sekiguchi |
| author_facet | Shiho Taniguchi Satoshi Nakayama Rin Iguchi Yasunori Sasakura Honoo Satake Shuichi Wada Nobuo Suzuki Michio Ogasawara Toshio Sekiguchi |
| author_sort | Shiho Taniguchi |
| collection | DOAJ |
| description | Abstract The cholecystokinin (CCK)/gastrin family peptides are involved in regulation of feeding and digestion in vertebrates. In the ascidian Ciona intestinalis type A (Ciona robusta), cionin, a CCK/gastrin family peptide, has been identified. Cionin is expressed exclusively in the central nervous system (CNS). In contrast, cionin receptor expression has been detected in the CNS, digestive tract, and ovary. Although cionin has been reported to be involved in ovulation, its physiological function in the CNS remains to be investigated. To elucidate its neural function, in the present study, we analyzed the expression of cionin and cionin receptors in the CNS. Cionin was expressed mainly in neurons residing in the anterior region of the cerebral ganglion. In contrast, the gene expressin of the cionin receptor gene CioR1, was detected in the middle part of the cerebral ganglion and showed a similar expression pattern to that of VACHT, a cholinergic neuron marker gene. Moreover, CioR1 was found to be expressed in cholinergic neurons. Consequently, these results suggest that cionin interacts with cholinergic neurons as a neurotransmitter or neuromodulator via CioR1. This study provides insights into a biological role of a CCK/gastrin family peptide in the CNS of ascidians. |
| first_indexed | 2024-04-24T23:07:50Z |
| format | Article |
| id | doaj.art-2f292e5893b04aebafb51065ddbdb494 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T23:07:50Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2f292e5893b04aebafb51065ddbdb4942024-03-17T12:22:46ZengNature PortfolioScientific Reports2045-23222024-03-011411810.1038/s41598-024-55908-7Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type AShiho Taniguchi0Satoshi Nakayama1Rin Iguchi2Yasunori Sasakura3Honoo Satake4Shuichi Wada5Nobuo Suzuki6Michio Ogasawara7Toshio Sekiguchi8Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa UniversityDepartment of Biology, Graduate School of Science, Chiba UniversityDepartment of Biology, Graduate School of Science, Chiba UniversityShimoda Marine Research Center, University of TsukubaBioorganic Research Institute, Suntory Foundation for Life SciencesDepartment of Animal Bioscience, Faculty of Bioscience, Nagahama Institute of Bio-Science and TechnologyNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa UniversityDepartment of Biology, Graduate School of Science, Chiba UniversityNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa UniversityAbstract The cholecystokinin (CCK)/gastrin family peptides are involved in regulation of feeding and digestion in vertebrates. In the ascidian Ciona intestinalis type A (Ciona robusta), cionin, a CCK/gastrin family peptide, has been identified. Cionin is expressed exclusively in the central nervous system (CNS). In contrast, cionin receptor expression has been detected in the CNS, digestive tract, and ovary. Although cionin has been reported to be involved in ovulation, its physiological function in the CNS remains to be investigated. To elucidate its neural function, in the present study, we analyzed the expression of cionin and cionin receptors in the CNS. Cionin was expressed mainly in neurons residing in the anterior region of the cerebral ganglion. In contrast, the gene expressin of the cionin receptor gene CioR1, was detected in the middle part of the cerebral ganglion and showed a similar expression pattern to that of VACHT, a cholinergic neuron marker gene. Moreover, CioR1 was found to be expressed in cholinergic neurons. Consequently, these results suggest that cionin interacts with cholinergic neurons as a neurotransmitter or neuromodulator via CioR1. This study provides insights into a biological role of a CCK/gastrin family peptide in the CNS of ascidians.https://doi.org/10.1038/s41598-024-55908-7CholecystokininGastrinAscidianCholinergic neuron |
| spellingShingle | Shiho Taniguchi Satoshi Nakayama Rin Iguchi Yasunori Sasakura Honoo Satake Shuichi Wada Nobuo Suzuki Michio Ogasawara Toshio Sekiguchi Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A Scientific Reports Cholecystokinin Gastrin Ascidian Cholinergic neuron |
| title | Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A |
| title_full | Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A |
| title_fullStr | Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A |
| title_full_unstemmed | Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A |
| title_short | Distribution of cionin, a cholecystokinin/gastrin family peptide, and its receptor in the central nervous system of Ciona intestinalis type A |
| title_sort | distribution of cionin a cholecystokinin gastrin family peptide and its receptor in the central nervous system of ciona intestinalis type a |
| topic | Cholecystokinin Gastrin Ascidian Cholinergic neuron |
| url | https://doi.org/10.1038/s41598-024-55908-7 |
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