Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development
Small molecules that can modulate or stabilize cell–cell interactions are valuable tools for investigating the impact of collective cell behavior on various biological processes such as development/morphogenesis, tissue regeneration and cancer progression. Recently, we showed that budesonide, a gluc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/7/1897 |
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| author | Filomena Amoroso Eduardo Ibello Federica Saracino Federica Cermola Giovanna Ponticelli Enrica Scalera Francesca Ricci Gino Villetti Gilda Cobellis Gabriella Minchiotti Eduardo Jorge Patriarca Dario De Cesare Cristina D’Aniello |
| author_facet | Filomena Amoroso Eduardo Ibello Federica Saracino Federica Cermola Giovanna Ponticelli Enrica Scalera Francesca Ricci Gino Villetti Gilda Cobellis Gabriella Minchiotti Eduardo Jorge Patriarca Dario De Cesare Cristina D’Aniello |
| author_sort | Filomena Amoroso |
| collection | DOAJ |
| description | Small molecules that can modulate or stabilize cell–cell interactions are valuable tools for investigating the impact of collective cell behavior on various biological processes such as development/morphogenesis, tissue regeneration and cancer progression. Recently, we showed that budesonide, a glucocorticoid widely used as an anti-asthmatic drug, is a potent regulator of stem cell pluripotency. Here we tested the effect of different budesonide derivatives and identified CHD-030498 as a more effective analogue of budesonide. CHD-030498 was able to prevent stem cell pluripotency exit in different cell-based models, including embryonic stem-to-mesenchymal transition, spontaneous differentiation and 3D gastruloid development, and at lower doses compared to budesonide. |
| first_indexed | 2024-03-11T00:43:44Z |
| format | Article |
| id | doaj.art-2f2a501481174f1b98902d8d9fa73017 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T00:43:44Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-2f2a501481174f1b98902d8d9fa730172023-11-18T20:55:22ZengMDPI AGPharmaceutics1999-49232023-07-01157189710.3390/pharmaceutics15071897Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid DevelopmentFilomena Amoroso0Eduardo Ibello1Federica Saracino2Federica Cermola3Giovanna Ponticelli4Enrica Scalera5Francesca Ricci6Gino Villetti7Gilda Cobellis8Gabriella Minchiotti9Eduardo Jorge Patriarca10Dario De Cesare11Cristina D’Aniello12Stem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalyStem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalyStem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalyStem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalyStem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalyExperimental Pharmacology & Translational Science Department, Corporate Pre-Clinical R&D, Chiesi Farmaceutici S.p.A., 43122 Parma, ItalyExperimental Pharmacology & Translational Science Department, Corporate Pre-Clinical R&D, Chiesi Farmaceutici S.p.A., 43122 Parma, ItalyExperimental Pharmacology & Translational Science Department, Corporate Pre-Clinical R&D, Chiesi Farmaceutici S.p.A., 43122 Parma, ItalyDepartment of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyStem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalyStem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalyStem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalyStem Cell Fate Laboratory, Institute of Genetics and Biophysics “A. Buzzati Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80131 Naples, ItalySmall molecules that can modulate or stabilize cell–cell interactions are valuable tools for investigating the impact of collective cell behavior on various biological processes such as development/morphogenesis, tissue regeneration and cancer progression. Recently, we showed that budesonide, a glucocorticoid widely used as an anti-asthmatic drug, is a potent regulator of stem cell pluripotency. Here we tested the effect of different budesonide derivatives and identified CHD-030498 as a more effective analogue of budesonide. CHD-030498 was able to prevent stem cell pluripotency exit in different cell-based models, including embryonic stem-to-mesenchymal transition, spontaneous differentiation and 3D gastruloid development, and at lower doses compared to budesonide.https://www.mdpi.com/1999-4923/15/7/1897drug toxicitybudesonide analoguesstem cellspluripotency exit3D gastruloids |
| spellingShingle | Filomena Amoroso Eduardo Ibello Federica Saracino Federica Cermola Giovanna Ponticelli Enrica Scalera Francesca Ricci Gino Villetti Gilda Cobellis Gabriella Minchiotti Eduardo Jorge Patriarca Dario De Cesare Cristina D’Aniello Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development Pharmaceutics drug toxicity budesonide analogues stem cells pluripotency exit 3D gastruloids |
| title | Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development |
| title_full | Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development |
| title_fullStr | Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development |
| title_full_unstemmed | Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development |
| title_short | Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development |
| title_sort | budesonide analogues preserve stem cell pluripotency and delay 3d gastruloid development |
| topic | drug toxicity budesonide analogues stem cells pluripotency exit 3D gastruloids |
| url | https://www.mdpi.com/1999-4923/15/7/1897 |
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