Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum
Glutamate receptors have been often associated with developmental processes. We used immunohistochemical techniques to evaluate the expression of the AMPA-type glutamate receptor (GluR) subunits in the chick optic tectum (TeO). Chick embryos from the 5th through the 20th embryonic day (E5-E20) and o...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2002-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800015 |
| _version_ | 1818364680979087360 |
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| author | Batista S.S. Pires R.S. Britto L.R.G. |
| author_facet | Batista S.S. Pires R.S. Britto L.R.G. |
| author_sort | Batista S.S. |
| collection | DOAJ |
| description | Glutamate receptors have been often associated with developmental processes. We used immunohistochemical techniques to evaluate the expression of the AMPA-type glutamate receptor (GluR) subunits in the chick optic tectum (TeO). Chick embryos from the 5th through the 20th embryonic day (E5-E20) and one-day-old (P1) chicks were used. The three types of immunoreactivity evaluated (GluR1, GluR2/3, and GluR4) had different temporal and spatial expression patterns in the several layers of the TeO. The GluR1 subunit first appeared as moderate staining on E7 and then increased on E9. The mature GluR1 pattern included intense staining only in layer 5 of the TeO. The GluR2/3 subunits presented low expression on E5, which became intense on E7. The staining for GluR2/3 changed to very intense on E14 in tectal layer 13. Staining of layer 13 neurons is the most prominent feature of GluR immunoreactivity in the adult TeO. The GluR4 subunit generally presented the lowest expression starting on E7, which was similar to the adult pattern. Some instances of transient expression of GluR subunits were observed in specific cell populations from E9 through E20. These results demonstrate a differential expression of the GluR subunits in the embryonic TeO, adding information about their possible functions in the developmental processes of the visual system. |
| first_indexed | 2024-12-13T22:08:14Z |
| format | Article |
| id | doaj.art-2f2aec74b6d24bbdbda0b63ebdcf1a31 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 0034-7310 |
| language | English |
| last_indexed | 2024-12-13T22:08:14Z |
| publishDate | 2002-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-2f2aec74b6d24bbdbda0b63ebdcf1a312022-12-21T23:29:46ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73102002-01-01358973978Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectumBatista S.S.Pires R.S.Britto L.R.G.Glutamate receptors have been often associated with developmental processes. We used immunohistochemical techniques to evaluate the expression of the AMPA-type glutamate receptor (GluR) subunits in the chick optic tectum (TeO). Chick embryos from the 5th through the 20th embryonic day (E5-E20) and one-day-old (P1) chicks were used. The three types of immunoreactivity evaluated (GluR1, GluR2/3, and GluR4) had different temporal and spatial expression patterns in the several layers of the TeO. The GluR1 subunit first appeared as moderate staining on E7 and then increased on E9. The mature GluR1 pattern included intense staining only in layer 5 of the TeO. The GluR2/3 subunits presented low expression on E5, which became intense on E7. The staining for GluR2/3 changed to very intense on E14 in tectal layer 13. Staining of layer 13 neurons is the most prominent feature of GluR immunoreactivity in the adult TeO. The GluR4 subunit generally presented the lowest expression starting on E7, which was similar to the adult pattern. Some instances of transient expression of GluR subunits were observed in specific cell populations from E9 through E20. These results demonstrate a differential expression of the GluR subunits in the embryonic TeO, adding information about their possible functions in the developmental processes of the visual system.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800015AMPADevelopmentGlutamate receptorsNeurotransmittersReceptor subunitsVisual system |
| spellingShingle | Batista S.S. Pires R.S. Britto L.R.G. Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum Brazilian Journal of Medical and Biological Research AMPA Development Glutamate receptors Neurotransmitters Receptor subunits Visual system |
| title | Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum |
| title_full | Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum |
| title_fullStr | Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum |
| title_full_unstemmed | Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum |
| title_short | Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum |
| title_sort | differential expression of ampa type glutamate receptor subunits during development of the chick optic tectum |
| topic | AMPA Development Glutamate receptors Neurotransmitters Receptor subunits Visual system |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800015 |
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