The Effects of Silver Sulfadiazine on Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms
Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), the most commonly detected drug-resistant microbe in hospitals, adheres to substrates and forms biofilms that are resistant to immunological responses and antimicrobial drugs. Currently, there is a need to develop alternative app...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/10/1551 |
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| author | Yutaka Ueda Motoyasu Miyazaki Kota Mashima Satoshi Takagi Shuuji Hara Hidetoshi Kamimura Shiro Jimi |
| author_facet | Yutaka Ueda Motoyasu Miyazaki Kota Mashima Satoshi Takagi Shuuji Hara Hidetoshi Kamimura Shiro Jimi |
| author_sort | Yutaka Ueda |
| collection | DOAJ |
| description | Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), the most commonly detected drug-resistant microbe in hospitals, adheres to substrates and forms biofilms that are resistant to immunological responses and antimicrobial drugs. Currently, there is a need to develop alternative approaches for treating infections caused by biofilms to prevent delays in wound healing. Silver has long been used as a disinfectant, which is non-specific and has relatively low cytotoxicity. Silver sulfadiazine (SSD) is a chemical complex clinically used for the prevention of wound infections after injury. However, its effects on biofilms are still unclear. In this study, we aimed to analyze the mechanisms underlying SSD action on biofilms formed by MRSA. The antibacterial effects of SSD were a result of silver ions and not sulfadiazine. Ionized silver from SSD in culture media was lower than that from silver nitrate; however, SSD, rather than silver nitrate, eradicated mature biofilms by bacterial killing. In SSD, sulfadiazine selectively bound to biofilms, and silver ions were then liberated. Consequently, the addition of an ion-chelator reduced the bactericidal effects of SSD on biofilms. These results indicate that SSD is an effective compound for the eradication of biofilms; thus, SSD should be used for the removal of biofilms formed on wounds. |
| first_indexed | 2024-03-10T15:46:29Z |
| format | Article |
| id | doaj.art-2f2c90a10cda4aafad8a2247b5943620 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:46:29Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2f2c90a10cda4aafad8a2247b59436202023-11-20T16:21:17ZengMDPI AGMicroorganisms2076-26072020-10-01810155110.3390/microorganisms8101551The Effects of Silver Sulfadiazine on Methicillin-Resistant <i>Staphylococcus aureus</i> BiofilmsYutaka Ueda0Motoyasu Miyazaki1Kota Mashima2Satoshi Takagi3Shuuji Hara4Hidetoshi Kamimura5Shiro Jimi6Department of Pharmacy, Fukuoka University Hospital, Fukuoka 814-0180, JapanDepartment of Pharmacy, Fukuoka University Chikushi Hospital, Fukuoka 818-8502, JapanDepartment of Pharmacy, Fukuoka University Hospital, Fukuoka 814-0180, JapanDepartments of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, JapanDepartment of Drug Informatics, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, JapanDepartment of Pharmacy, Fukuoka University Hospital, Fukuoka 814-0180, JapanCentral Lab for Pathology and Morphology, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, JapanMethicillin-resistant <i>Staphylococcus aureus</i> (MRSA), the most commonly detected drug-resistant microbe in hospitals, adheres to substrates and forms biofilms that are resistant to immunological responses and antimicrobial drugs. Currently, there is a need to develop alternative approaches for treating infections caused by biofilms to prevent delays in wound healing. Silver has long been used as a disinfectant, which is non-specific and has relatively low cytotoxicity. Silver sulfadiazine (SSD) is a chemical complex clinically used for the prevention of wound infections after injury. However, its effects on biofilms are still unclear. In this study, we aimed to analyze the mechanisms underlying SSD action on biofilms formed by MRSA. The antibacterial effects of SSD were a result of silver ions and not sulfadiazine. Ionized silver from SSD in culture media was lower than that from silver nitrate; however, SSD, rather than silver nitrate, eradicated mature biofilms by bacterial killing. In SSD, sulfadiazine selectively bound to biofilms, and silver ions were then liberated. Consequently, the addition of an ion-chelator reduced the bactericidal effects of SSD on biofilms. These results indicate that SSD is an effective compound for the eradication of biofilms; thus, SSD should be used for the removal of biofilms formed on wounds.https://www.mdpi.com/2076-2607/8/10/1551biofilmMRSAsilver ionsilver sulfadiazinewound infections |
| spellingShingle | Yutaka Ueda Motoyasu Miyazaki Kota Mashima Satoshi Takagi Shuuji Hara Hidetoshi Kamimura Shiro Jimi The Effects of Silver Sulfadiazine on Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms Microorganisms biofilm MRSA silver ion silver sulfadiazine wound infections |
| title | The Effects of Silver Sulfadiazine on Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms |
| title_full | The Effects of Silver Sulfadiazine on Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms |
| title_fullStr | The Effects of Silver Sulfadiazine on Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms |
| title_full_unstemmed | The Effects of Silver Sulfadiazine on Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms |
| title_short | The Effects of Silver Sulfadiazine on Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms |
| title_sort | effects of silver sulfadiazine on methicillin resistant i staphylococcus aureus i biofilms |
| topic | biofilm MRSA silver ion silver sulfadiazine wound infections |
| url | https://www.mdpi.com/2076-2607/8/10/1551 |
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