Molecular Characterization and Expression Analysis of the Peroxisome Proliferator Activated Receptor Delta () Gene before and after Exercise in Horse
While athletic abilities such as speed, endurance and recovery are important in the horse, genes related to these abilities have not been extensively investigated. Here, we characterized the horse peroxisome proliferator-activated receptor delta (PPARδ) gene and analyzed the expression of PPARδ duri...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2015-05-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-28-5-697.pdf |
| _version_ | 1818108543239192576 |
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| author | Hyun-Woo Cho Sangsu Shin Jeong-Woong Park Jae-Young Choi Nam-Young Kim Woon-Kyu Lee Hak-Kyo Lee Ki-Duk Song Byung-Wook Cho |
| author_facet | Hyun-Woo Cho Sangsu Shin Jeong-Woong Park Jae-Young Choi Nam-Young Kim Woon-Kyu Lee Hak-Kyo Lee Ki-Duk Song Byung-Wook Cho |
| author_sort | Hyun-Woo Cho |
| collection | DOAJ |
| description | While athletic abilities such as speed, endurance and recovery are important in the horse, genes related to these abilities have not been extensively investigated. Here, we characterized the horse peroxisome proliferator-activated receptor delta (PPARδ) gene and analyzed the expression of PPARδ during exercise. PPARδ is a known regulator of β-oxidation, muscle fiber transformation, and running endurance. Through evolutionary analysis using the synonymous and non-synonymous mutation ratio, it was revealed that positive selection occurred in the horse PPARδ gene. Two important domains related to nuclear hormone receptors, C4 zinc finger and ligand binding domain, were also found to be conserved well in horse PPARδ. Horse PPARδ was expressed ubiquitously in many tissues, but the expression level was various depending on the tissues. In the skeletal muscle, PPARδ increased about 2.5 folds after 30 min of exercise. Unlike in muscle, the increase of PPARδ expression was observed at 60 min but not 30 min of exercise in leukocytes. This finding might be useful for testing the endurance of horse using blood samples. Conclusively, the horse PPARδ gene is evolutionarily conserved well and can be used as a biomarker of endurance in horse. |
| first_indexed | 2024-12-11T02:17:02Z |
| format | Article |
| id | doaj.art-2f2d17272d74416db2afa753ef405ece |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-11T02:17:02Z |
| publishDate | 2015-05-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-2f2d17272d74416db2afa753ef405ece2022-12-22T01:24:10ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172015-05-0128569770210.5713/ajas.14.057523077Molecular Characterization and Expression Analysis of the Peroxisome Proliferator Activated Receptor Delta () Gene before and after Exercise in HorseHyun-Woo ChoSangsu Shin0Jeong-Woong ParkJae-Young ChoiNam-Young Kim1Woon-Kyu Lee2Hak-Kyo Lee3Ki-Duk Song4Byung-Wook Cho Life and Industry Convergence Research Institute, College of Natural Resource and Life Science, Pusan National University, Miryang 627-706, .Korea Subtropical Animal Experiment Station, National Institute of Animal Science, RDA, Jeju 690-150, .Korea College of Medicine, Inha University, Incheon 400-712, .Korea Genomic Informatics Center, HanKyong National University, Anseong 456-749, .Korea Genomic Informatics Center, HanKyong National University, Anseong 456-749, .KoreaWhile athletic abilities such as speed, endurance and recovery are important in the horse, genes related to these abilities have not been extensively investigated. Here, we characterized the horse peroxisome proliferator-activated receptor delta (PPARδ) gene and analyzed the expression of PPARδ during exercise. PPARδ is a known regulator of β-oxidation, muscle fiber transformation, and running endurance. Through evolutionary analysis using the synonymous and non-synonymous mutation ratio, it was revealed that positive selection occurred in the horse PPARδ gene. Two important domains related to nuclear hormone receptors, C4 zinc finger and ligand binding domain, were also found to be conserved well in horse PPARδ. Horse PPARδ was expressed ubiquitously in many tissues, but the expression level was various depending on the tissues. In the skeletal muscle, PPARδ increased about 2.5 folds after 30 min of exercise. Unlike in muscle, the increase of PPARδ expression was observed at 60 min but not 30 min of exercise in leukocytes. This finding might be useful for testing the endurance of horse using blood samples. Conclusively, the horse PPARδ gene is evolutionarily conserved well and can be used as a biomarker of endurance in horse.http://www.ajas.info/upload/pdf/ajas-28-5-697.pdfHorsePeroxisome Proliferator-activated Receptor DeltaExerciseEndurance |
| spellingShingle | Hyun-Woo Cho Sangsu Shin Jeong-Woong Park Jae-Young Choi Nam-Young Kim Woon-Kyu Lee Hak-Kyo Lee Ki-Duk Song Byung-Wook Cho Molecular Characterization and Expression Analysis of the Peroxisome Proliferator Activated Receptor Delta () Gene before and after Exercise in Horse Asian-Australasian Journal of Animal Sciences Horse Peroxisome Proliferator-activated Receptor Delta Exercise Endurance |
| title | Molecular Characterization and Expression Analysis of the Peroxisome Proliferator Activated Receptor Delta () Gene before and after Exercise in Horse |
| title_full | Molecular Characterization and Expression Analysis of the Peroxisome Proliferator Activated Receptor Delta () Gene before and after Exercise in Horse |
| title_fullStr | Molecular Characterization and Expression Analysis of the Peroxisome Proliferator Activated Receptor Delta () Gene before and after Exercise in Horse |
| title_full_unstemmed | Molecular Characterization and Expression Analysis of the Peroxisome Proliferator Activated Receptor Delta () Gene before and after Exercise in Horse |
| title_short | Molecular Characterization and Expression Analysis of the Peroxisome Proliferator Activated Receptor Delta () Gene before and after Exercise in Horse |
| title_sort | molecular characterization and expression analysis of the peroxisome proliferator activated receptor delta gene before and after exercise in horse |
| topic | Horse Peroxisome Proliferator-activated Receptor Delta Exercise Endurance |
| url | http://www.ajas.info/upload/pdf/ajas-28-5-697.pdf |
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