Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach
Acquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and sp...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/14/2/252 |
| _version_ | 1827653277537271808 |
|---|---|
| author | Silvia Pisani Stefania Croce Simone Mauramati Marta Marmonti Lorenzo Cobianchi Irene Herman Rossella Dorati Maria Antonietta Avanzini Ida Genta Marco Benazzo Bice Conti |
| author_facet | Silvia Pisani Stefania Croce Simone Mauramati Marta Marmonti Lorenzo Cobianchi Irene Herman Rossella Dorati Maria Antonietta Avanzini Ida Genta Marco Benazzo Bice Conti |
| author_sort | Silvia Pisani |
| collection | DOAJ |
| description | Acquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and speed up the regeneration process, ensuring adequate support and esophageal tissue reconstruction and avoiding the use of autologous conduits. Copolymers poly-L-lactide-co-poly-ε-caprolactone (PLA-PCL) 70:30 and 85:15 ratio were chosen to prepare electrospun tubular scaffolds. Electrospinning apparatus equipped with two different types of tubular mandrels: cylindrical (∅ 10 mm) and asymmetrical (∅ 10 mm and ∅ 8 mm) were used. Tubular scaffolds underwent morphological, mechanical (uniaxial tensile stress) and biological (MTT and Dapi staining) characterization. Asymmetric tubular geometry resulted in the best properties and was selected for in vivo surgical implantation. Anesthetized pigs underwent full thickness circumferential resection of the mid-lower thoracic esophagus, followed by implantation of the asymmetric scaffold. Preliminary in vivo results demonstrated that detached stitch suture achieved better results in terms of animal welfare and scaffold integration; thus, it is to be preferred to continuous suture. |
| first_indexed | 2024-03-09T21:14:46Z |
| format | Article |
| id | doaj.art-2f2fc9ca8c3141c1a3d2bbf13a8a2549 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T21:14:46Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-2f2fc9ca8c3141c1a3d2bbf13a8a25492023-11-23T21:36:11ZengMDPI AGPharmaceutics1999-49232022-01-0114225210.3390/pharmaceutics14020252Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo ApproachSilvia Pisani0Stefania Croce1Simone Mauramati2Marta Marmonti3Lorenzo Cobianchi4Irene Herman5Rossella Dorati6Maria Antonietta Avanzini7Ida Genta8Marco Benazzo9Bice Conti10Otorhinolaryngology Unit, Department of Surgical Sciences, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyDepartment of Clinical, Surgical, Diagnostic & Pediatric Sciences, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyOtorhinolaryngology Unit, Department of Surgical Sciences, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Clinical, Surgical, Diagnostic & Pediatric Sciences, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyOtorhinolaryngology Unit, Department of Surgical Sciences, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Paediatric Oncoaematology, IRCCS Policlinico S. Matteo, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyOtorhinolaryngology Unit, Department of Surgical Sciences, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyAcquired congenital esophageal malformations, such as malignant esophageal cancer, require esophagectomy resulting in full thickness resection, which cannot be left untreated. The proposed approach is a polymeric full-thickness scaffold engineered with mesenchymal stem cells (MSCs) to promote and speed up the regeneration process, ensuring adequate support and esophageal tissue reconstruction and avoiding the use of autologous conduits. Copolymers poly-L-lactide-co-poly-ε-caprolactone (PLA-PCL) 70:30 and 85:15 ratio were chosen to prepare electrospun tubular scaffolds. Electrospinning apparatus equipped with two different types of tubular mandrels: cylindrical (∅ 10 mm) and asymmetrical (∅ 10 mm and ∅ 8 mm) were used. Tubular scaffolds underwent morphological, mechanical (uniaxial tensile stress) and biological (MTT and Dapi staining) characterization. Asymmetric tubular geometry resulted in the best properties and was selected for in vivo surgical implantation. Anesthetized pigs underwent full thickness circumferential resection of the mid-lower thoracic esophagus, followed by implantation of the asymmetric scaffold. Preliminary in vivo results demonstrated that detached stitch suture achieved better results in terms of animal welfare and scaffold integration; thus, it is to be preferred to continuous suture.https://www.mdpi.com/1999-4923/14/2/252tissue engineeringelectrospun nanofibersesophagus regenerationtubular scaffoldmesenchymal stem cellin vivo implantation |
| spellingShingle | Silvia Pisani Stefania Croce Simone Mauramati Marta Marmonti Lorenzo Cobianchi Irene Herman Rossella Dorati Maria Antonietta Avanzini Ida Genta Marco Benazzo Bice Conti Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach Pharmaceutics tissue engineering electrospun nanofibers esophagus regeneration tubular scaffold mesenchymal stem cell in vivo implantation |
| title | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
| title_full | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
| title_fullStr | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
| title_full_unstemmed | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
| title_short | Engineered Full Thickness Electrospun Scaffold for Esophageal Tissue Regeneration: From In Vitro to In Vivo Approach |
| title_sort | engineered full thickness electrospun scaffold for esophageal tissue regeneration from in vitro to in vivo approach |
| topic | tissue engineering electrospun nanofibers esophagus regeneration tubular scaffold mesenchymal stem cell in vivo implantation |
| url | https://www.mdpi.com/1999-4923/14/2/252 |
| work_keys_str_mv | AT silviapisani engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT stefaniacroce engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT simonemauramati engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT martamarmonti engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT lorenzocobianchi engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT ireneherman engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT rosselladorati engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT mariaantoniettaavanzini engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT idagenta engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT marcobenazzo engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach AT biceconti engineeredfullthicknesselectrospunscaffoldforesophagealtissueregenerationfrominvitrotoinvivoapproach |