Reflections on the Biology of Cell Culture Models: Living on the Edge of Oxidative Metabolism in Cancer Cells
Nowadays, the study of cell metabolism is a hot topic in cancer research. Many studies have used 2D conventional cell cultures for their simplicity and the facility to infer mechanisms. However, the limitations of bidimensional cell cultures to recreate architecture, mechanics, and cell communicatio...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/3/2717 |
| _version_ | 1797624282845544448 |
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| author | Alba Moran-Alvarez Pedro Gonzalez-Menendez Juan C. Mayo Rosa M. Sainz |
| author_facet | Alba Moran-Alvarez Pedro Gonzalez-Menendez Juan C. Mayo Rosa M. Sainz |
| author_sort | Alba Moran-Alvarez |
| collection | DOAJ |
| description | Nowadays, the study of cell metabolism is a hot topic in cancer research. Many studies have used 2D conventional cell cultures for their simplicity and the facility to infer mechanisms. However, the limitations of bidimensional cell cultures to recreate architecture, mechanics, and cell communication between tumor cells and their environment, have forced the development of other more realistic in vitro methodologies. Therefore, the explosion of 3D culture techniques and the necessity to reduce animal experimentation to a minimum has attracted the attention of researchers in the field of cancer metabolism. Here, we revise the limitations of actual culture models and discuss the utility of several 3D culture techniques to resolve those limitations. |
| first_indexed | 2024-03-11T09:40:39Z |
| format | Article |
| id | doaj.art-2f309e101ce445fa93e9a6e69edfe4b0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:40:39Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2f309e101ce445fa93e9a6e69edfe4b02023-11-16T17:01:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01243271710.3390/ijms24032717Reflections on the Biology of Cell Culture Models: Living on the Edge of Oxidative Metabolism in Cancer CellsAlba Moran-Alvarez0Pedro Gonzalez-Menendez1Juan C. Mayo2Rosa M. Sainz3Departamento de Morfología y Biología Celular, School of Medicine, Julián Claveria 6, 33006 Oviedo, SpainDepartamento de Morfología y Biología Celular, School of Medicine, Julián Claveria 6, 33006 Oviedo, SpainDepartamento de Morfología y Biología Celular, School of Medicine, Julián Claveria 6, 33006 Oviedo, SpainDepartamento de Morfología y Biología Celular, School of Medicine, Julián Claveria 6, 33006 Oviedo, SpainNowadays, the study of cell metabolism is a hot topic in cancer research. Many studies have used 2D conventional cell cultures for their simplicity and the facility to infer mechanisms. However, the limitations of bidimensional cell cultures to recreate architecture, mechanics, and cell communication between tumor cells and their environment, have forced the development of other more realistic in vitro methodologies. Therefore, the explosion of 3D culture techniques and the necessity to reduce animal experimentation to a minimum has attracted the attention of researchers in the field of cancer metabolism. Here, we revise the limitations of actual culture models and discuss the utility of several 3D culture techniques to resolve those limitations.https://www.mdpi.com/1422-0067/24/3/2717metabolismredox signalingcell signalingcell culture3D |
| spellingShingle | Alba Moran-Alvarez Pedro Gonzalez-Menendez Juan C. Mayo Rosa M. Sainz Reflections on the Biology of Cell Culture Models: Living on the Edge of Oxidative Metabolism in Cancer Cells International Journal of Molecular Sciences metabolism redox signaling cell signaling cell culture 3D |
| title | Reflections on the Biology of Cell Culture Models: Living on the Edge of Oxidative Metabolism in Cancer Cells |
| title_full | Reflections on the Biology of Cell Culture Models: Living on the Edge of Oxidative Metabolism in Cancer Cells |
| title_fullStr | Reflections on the Biology of Cell Culture Models: Living on the Edge of Oxidative Metabolism in Cancer Cells |
| title_full_unstemmed | Reflections on the Biology of Cell Culture Models: Living on the Edge of Oxidative Metabolism in Cancer Cells |
| title_short | Reflections on the Biology of Cell Culture Models: Living on the Edge of Oxidative Metabolism in Cancer Cells |
| title_sort | reflections on the biology of cell culture models living on the edge of oxidative metabolism in cancer cells |
| topic | metabolism redox signaling cell signaling cell culture 3D |
| url | https://www.mdpi.com/1422-0067/24/3/2717 |
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