16S-23S rRNA Internal Transcribed Spacer Region (<i>ITS</i>) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating <i>Lactococcus garvieae</i> and <i>Lactococcus petauri</i>
<i>Lactococcus garvieae</i> is the etiological agent of lactococcosis, a clinically and economically significant infectious disease affecting farmed rainbow trout. <i>L. garvieae</i> had been considered the only cause of lactococcosis for a long time; however, <i>L. pet...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1320 |
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| author | Nadia Stoppani Silvia Colussi Paolo Pastorino Marino Prearo Simona Sciuto Ilhan Altinok Rafet Çağrı Öztürk Mustafa Ture Ana Isabel Vela Maria del Mar Blanco Charalampos Kotzamanidis Konstantina Bitchava Andigoni Malousi Lucio Fariano Donatella Volpatti Pier Luigi Acutis Jose Francisco Fernández-Garayzábal |
| author_facet | Nadia Stoppani Silvia Colussi Paolo Pastorino Marino Prearo Simona Sciuto Ilhan Altinok Rafet Çağrı Öztürk Mustafa Ture Ana Isabel Vela Maria del Mar Blanco Charalampos Kotzamanidis Konstantina Bitchava Andigoni Malousi Lucio Fariano Donatella Volpatti Pier Luigi Acutis Jose Francisco Fernández-Garayzábal |
| author_sort | Nadia Stoppani |
| collection | DOAJ |
| description | <i>Lactococcus garvieae</i> is the etiological agent of lactococcosis, a clinically and economically significant infectious disease affecting farmed rainbow trout. <i>L. garvieae</i> had been considered the only cause of lactococcosis for a long time; however, <i>L. petauri</i>, another species of the genus <i>Lactococcus</i>, has lately been linked to the same disease. The genomes and biochemical profiles of <i>L. petauri</i> and <i>L. garvieae</i> have a high degree of similarity. Traditional diagnostic tests currently available cannot distinguish between these two species. The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate <i>L. garvieae</i> from <i>L. petauri</i>, saving time and money compared to genomics methods currently used as diagnostic tools for accurate discrimination between these two species. The <i>ITS</i> region of 82 strains was amplified and sequenced. The amplified fragments varied in size from 500 to 550 bp. Based on the sequence, seven SNPs were identified that separate <i>L. garvieae</i> from <i>L. petauri</i>. The 16S-23S rRNA ITS region has enough resolution to distinguish between closely related <i>L. garvieae</i> and <i>L. petauri</i> and it can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak. |
| first_indexed | 2024-03-11T03:28:09Z |
| format | Article |
| id | doaj.art-2f31eb41797b4f458bfeff8f292def2c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T03:28:09Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2f31eb41797b4f458bfeff8f292def2c2023-11-18T02:35:03ZengMDPI AGMicroorganisms2076-26072023-05-01115132010.3390/microorganisms1105132016S-23S rRNA Internal Transcribed Spacer Region (<i>ITS</i>) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating <i>Lactococcus garvieae</i> and <i>Lactococcus petauri</i>Nadia Stoppani0Silvia Colussi1Paolo Pastorino2Marino Prearo3Simona Sciuto4Ilhan Altinok5Rafet Çağrı Öztürk6Mustafa Ture7Ana Isabel Vela8Maria del Mar Blanco9Charalampos Kotzamanidis10Konstantina Bitchava11Andigoni Malousi12Lucio Fariano13Donatella Volpatti14Pier Luigi Acutis15Jose Francisco Fernández-Garayzábal16Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyFaculty of Marine Sciences, Karadeniz Technical University, Sürmene, 61530 Trabzon, TurkeyFaculty of Marine Sciences, Karadeniz Technical University, Sürmene, 61530 Trabzon, TurkeyCentral Fisheries Research Institute (SUMAE), 61250 Trabzon, TurkeyVISAVET and Department of Animal Health, Universidad Complutense de Madrid, 28040 Madrid, SpainVISAVET and Department of Animal Health, Universidad Complutense de Madrid, 28040 Madrid, SpainVeterinary Research Institute, ELGO-DIMITRA, 54124 Thessaloniki, GreeceSchool of Animal Biosciences, Agricultural University of Athens, 11855 Athens, GreeceLaboratory of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceAzienda Agricola Canali Cavour, 12044 Centallo, ItalyDepartment of Agricultural, Food, Environmental and Animal Sciences (DI4A), University of Udine, 33100 Udine, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyVISAVET and Department of Animal Health, Universidad Complutense de Madrid, 28040 Madrid, Spain<i>Lactococcus garvieae</i> is the etiological agent of lactococcosis, a clinically and economically significant infectious disease affecting farmed rainbow trout. <i>L. garvieae</i> had been considered the only cause of lactococcosis for a long time; however, <i>L. petauri</i>, another species of the genus <i>Lactococcus</i>, has lately been linked to the same disease. The genomes and biochemical profiles of <i>L. petauri</i> and <i>L. garvieae</i> have a high degree of similarity. Traditional diagnostic tests currently available cannot distinguish between these two species. The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate <i>L. garvieae</i> from <i>L. petauri</i>, saving time and money compared to genomics methods currently used as diagnostic tools for accurate discrimination between these two species. The <i>ITS</i> region of 82 strains was amplified and sequenced. The amplified fragments varied in size from 500 to 550 bp. Based on the sequence, seven SNPs were identified that separate <i>L. garvieae</i> from <i>L. petauri</i>. The 16S-23S rRNA ITS region has enough resolution to distinguish between closely related <i>L. garvieae</i> and <i>L. petauri</i> and it can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.https://www.mdpi.com/2076-2607/11/5/1320<i>Lactococcus garvieae</i><i>Lactococcus petauri</i>genome16S-23S internal transcribed spacer regiondiagnostic techniquelactococcosis |
| spellingShingle | Nadia Stoppani Silvia Colussi Paolo Pastorino Marino Prearo Simona Sciuto Ilhan Altinok Rafet Çağrı Öztürk Mustafa Ture Ana Isabel Vela Maria del Mar Blanco Charalampos Kotzamanidis Konstantina Bitchava Andigoni Malousi Lucio Fariano Donatella Volpatti Pier Luigi Acutis Jose Francisco Fernández-Garayzábal 16S-23S rRNA Internal Transcribed Spacer Region (<i>ITS</i>) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating <i>Lactococcus garvieae</i> and <i>Lactococcus petauri</i> Microorganisms <i>Lactococcus garvieae</i> <i>Lactococcus petauri</i> genome 16S-23S internal transcribed spacer region diagnostic technique lactococcosis |
| title | 16S-23S rRNA Internal Transcribed Spacer Region (<i>ITS</i>) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating <i>Lactococcus garvieae</i> and <i>Lactococcus petauri</i> |
| title_full | 16S-23S rRNA Internal Transcribed Spacer Region (<i>ITS</i>) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating <i>Lactococcus garvieae</i> and <i>Lactococcus petauri</i> |
| title_fullStr | 16S-23S rRNA Internal Transcribed Spacer Region (<i>ITS</i>) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating <i>Lactococcus garvieae</i> and <i>Lactococcus petauri</i> |
| title_full_unstemmed | 16S-23S rRNA Internal Transcribed Spacer Region (<i>ITS</i>) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating <i>Lactococcus garvieae</i> and <i>Lactococcus petauri</i> |
| title_short | 16S-23S rRNA Internal Transcribed Spacer Region (<i>ITS</i>) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating <i>Lactococcus garvieae</i> and <i>Lactococcus petauri</i> |
| title_sort | 16s 23s rrna internal transcribed spacer region i its i sequencing a potential molecular diagnostic tool for differentiating i lactococcus garvieae i and i lactococcus petauri i |
| topic | <i>Lactococcus garvieae</i> <i>Lactococcus petauri</i> genome 16S-23S internal transcribed spacer region diagnostic technique lactococcosis |
| url | https://www.mdpi.com/2076-2607/11/5/1320 |
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