Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 Cells
Abstract Background & aim: Several studies have reported anti-cancer properties of sagebrush plain. The aim of this study was to evaluate the cytotoxicity of the methanol extract of sagebrush plain on human breast cancer MCF7 cells. Methods: In the present experimental study, the toxic effect...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2013-07-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-230&slc_lang=en&sid=1 |
| _version_ | 1811236680077672448 |
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| author | B Gordanian M Behbahani J Carapetian M Fazilati |
| author_facet | B Gordanian M Behbahani J Carapetian M Fazilati |
| author_sort | B Gordanian |
| collection | DOAJ |
| description | Abstract
Background & aim: Several studies have reported anti-cancer properties of sagebrush plain. The aim of this study was to evaluate the cytotoxicity of the methanol extract of sagebrush plain on human breast cancer MCF7 cells.
Methods: In the present experimental study, the toxic effects of methanol extracts of flowers, leaves, stems and roots of sagebrush plain from of Khorassan and Esfahan province were tested on human breast cancer cells MCF-7 and normal cells HEK293 . Plant samples were extracted by methanol and their toxic effects on normal and breast cancer cells at concentrations of 5.62, 125, 250 and 500 µg/ml was determined by MTT. Both breast cancer cells MCF-7 and normal HEK293 cells were cultured in RPMI-1640 medium and DMEM containing 10% fetal calf serums were cultured. Data were analyzed by one-way ANOVA.
Results: The methanol extract of sagebrush showed toxicity on MCF7 cells. The extract of Khorasan showed higher toxicity than Esfahan province. IC50 of sagebrush plant for all parts of the plant were obtained more than 500 µg/ml, but the IC50 of sagebrush plant of Khorasan region in leaf and flower were 205 ± 1.3 and 213 ± 5.3µg respectively. The leaves and flowers in both cases had the highest cytotoxicity. Plant extracts in both regions did not show significant cytotoxicity on normal HEK293 cells.
Conclusion: The extract of the sagebrush plain region of Khorasan region showed greater cytotoxicity than Esfahan. It seems that different environmental conditionshas considerable cytotoxicity.
Keywords: Sagebrush Plain, MTT, Breast Cancer |
| first_indexed | 2024-04-12T12:13:17Z |
| format | Article |
| id | doaj.art-2f3387bcb739470e8a23c37ed846cc2d |
| institution | Directory Open Access Journal |
| issn | 1728-6506 1728-6514 |
| language | fas |
| last_indexed | 2024-04-12T12:13:17Z |
| publishDate | 2013-07-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-2f3387bcb739470e8a23c37ed846cc2d2022-12-22T03:33:31ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65061728-65142013-07-01183241251Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 CellsB Gordanian0M Behbahani1J Carapetian2M Fazilati3 Abstract Background & aim: Several studies have reported anti-cancer properties of sagebrush plain. The aim of this study was to evaluate the cytotoxicity of the methanol extract of sagebrush plain on human breast cancer MCF7 cells. Methods: In the present experimental study, the toxic effects of methanol extracts of flowers, leaves, stems and roots of sagebrush plain from of Khorassan and Esfahan province were tested on human breast cancer cells MCF-7 and normal cells HEK293 . Plant samples were extracted by methanol and their toxic effects on normal and breast cancer cells at concentrations of 5.62, 125, 250 and 500 µg/ml was determined by MTT. Both breast cancer cells MCF-7 and normal HEK293 cells were cultured in RPMI-1640 medium and DMEM containing 10% fetal calf serums were cultured. Data were analyzed by one-way ANOVA. Results: The methanol extract of sagebrush showed toxicity on MCF7 cells. The extract of Khorasan showed higher toxicity than Esfahan province. IC50 of sagebrush plant for all parts of the plant were obtained more than 500 µg/ml, but the IC50 of sagebrush plant of Khorasan region in leaf and flower were 205 ± 1.3 and 213 ± 5.3µg respectively. The leaves and flowers in both cases had the highest cytotoxicity. Plant extracts in both regions did not show significant cytotoxicity on normal HEK293 cells. Conclusion: The extract of the sagebrush plain region of Khorasan region showed greater cytotoxicity than Esfahan. It seems that different environmental conditionshas considerable cytotoxicity. Keywords: Sagebrush Plain, MTT, Breast Cancerhttp://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-230&slc_lang=en&sid=1Keywords: Sagebrush Plain MTT Breast Cancer |
| spellingShingle | B Gordanian M Behbahani J Carapetian M Fazilati Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 Cells Armaghane Danesh Bimonthly Journal Keywords: Sagebrush Plain MTT Breast Cancer |
| title | Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 Cells |
| title_full | Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 Cells |
| title_fullStr | Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 Cells |
| title_full_unstemmed | Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 Cells |
| title_short | Evaluation of Cytotoxicity of Sagebrush Plain Extract on Human Breast Cancer MCF7 Cells |
| title_sort | evaluation of cytotoxicity of sagebrush plain extract on human breast cancer mcf7 cells |
| topic | Keywords: Sagebrush Plain MTT Breast Cancer |
| url | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-230&slc_lang=en&sid=1 |
| work_keys_str_mv | AT bgordanian evaluationofcytotoxicityofsagebrushplainextractonhumanbreastcancermcf7cells AT mbehbahani evaluationofcytotoxicityofsagebrushplainextractonhumanbreastcancermcf7cells AT jcarapetian evaluationofcytotoxicityofsagebrushplainextractonhumanbreastcancermcf7cells AT mfazilati evaluationofcytotoxicityofsagebrushplainextractonhumanbreastcancermcf7cells |