Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre
This study was aimed to identify possible critical points of Escherichia coli (E. coli ) O157:H7, a pathogenic agent, contamination in aceh cattle breeding centre. For this purpose, samples were collected from cattle faeces, hand of workers (animal keepers), and water sources in the farm using cross...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2020-01-01
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| Series: | E3S Web of Conferences |
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| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01021.pdf |
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| author | Ferasyi Teuku R. Abrar Mahdi Subianto Muhammad Afrianandra Cut Hambal Muhammad Razali Razali Ismail Ismail Nurliana Nurliana Rastina Rastina Sari Wahyu E. Safika Safika Vierman Vierman Mutia Nellita Barus Rina A. Yusmadi Yusmadi Rosa Teuku S. Ramadhan Rezky |
| author_facet | Ferasyi Teuku R. Abrar Mahdi Subianto Muhammad Afrianandra Cut Hambal Muhammad Razali Razali Ismail Ismail Nurliana Nurliana Rastina Rastina Sari Wahyu E. Safika Safika Vierman Vierman Mutia Nellita Barus Rina A. Yusmadi Yusmadi Rosa Teuku S. Ramadhan Rezky |
| author_sort | Ferasyi Teuku R. |
| collection | DOAJ |
| description | This study was aimed to identify possible critical points of Escherichia coli (E. coli ) O157:H7, a pathogenic agent, contamination in aceh cattle breeding centre. For this purpose, samples were collected from cattle faeces, hand of workers (animal keepers), and water sources in the farm using cross-sectional approach. A number of 85 samples of cattle faecal swab were collected randomly from the animals in the breeding centre. The samples of swab of hand of all workers (15 persons) were collected before and after work. Then, the water sources from 11 cattle house locations in the breeding centre were collected. The water sources were divided into three different locations, namely the water containers, taps, and water puddle on the floors. At each source a number of 11 samples were collected. Isolation of E. coli was conducted on Eosin Methylene Blue Agar (EMBA), followed by identification on Sorbitol MacConkey Agar (SMAC). Then Molecular subtyping of E. coli O157:H7 genes was conducted using multiplex-PCR analysis. Data were analysed descriptively. The results of this study showed that 72 samples (85 %) among 85 samples were positive for E. coli and the rest of samples were positive for other types of bacteria. Sample isolation from swabs of hand was found 3 positive E. coli before work and 1 positive E. coli after work from 15 workers. The most potential water sources for E. coli contamination were the water in taps, and water puddle on the floor of cattle houses. Then, two of samples of E. coli isolated from rectal swab were confirmed as E. coli O157:H7 using PCR test, based on the presence of stx2 gene. In conclusion, the risk of presence of E. coli as zoonotic agents of E. coli O157:H7 in aceh cattle as well as from the farm workers and surrounding area are high. An appropriate control strategy is needed to apply in the aceh cattle farm to prevent from E. coli O157:H7 outbreak in the future. |
| first_indexed | 2024-12-17T03:48:15Z |
| format | Article |
| id | doaj.art-2f363f7f7ca44b7fbd7f037428a80381 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-17T03:48:15Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-2f363f7f7ca44b7fbd7f037428a803812022-12-21T22:04:50ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011510102110.1051/e3sconf/202015101021e3sconf_icvaes2020_01021Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding CentreFerasyi Teuku R.Abrar MahdiSubianto Muhammad0Afrianandra Cut1Hambal MuhammadRazali RazaliIsmail IsmailNurliana NurlianaRastina RastinaSari Wahyu E.Safika Safika2Vierman Vierman3Mutia Nellita4Barus Rina A.5Yusmadi Yusmadi6Rosa Teuku S.7Ramadhan Rezky8Faculty of Mathematics and Natural Sciences of Syiah Kuala UniversityFaculty of Economics and Bussiness of Syiah Kuala UniversityFaculty of Veterinary Medicine of IPB UniversityLivestock Breeding and Fodder Centre (BPTU-HPT)Livestock Breeding and Fodder Centre (BPTU-HPT)Livestock Breeding and Fodder Centre (BPTU-HPT)Livestock Breeding and Fodder Centre (BPTU-HPT)Centre for Tropical Veterinary Studies of Syiah Kuala UniversityCentre for Tropical Veterinary Studies of Syiah Kuala UniversityThis study was aimed to identify possible critical points of Escherichia coli (E. coli ) O157:H7, a pathogenic agent, contamination in aceh cattle breeding centre. For this purpose, samples were collected from cattle faeces, hand of workers (animal keepers), and water sources in the farm using cross-sectional approach. A number of 85 samples of cattle faecal swab were collected randomly from the animals in the breeding centre. The samples of swab of hand of all workers (15 persons) were collected before and after work. Then, the water sources from 11 cattle house locations in the breeding centre were collected. The water sources were divided into three different locations, namely the water containers, taps, and water puddle on the floors. At each source a number of 11 samples were collected. Isolation of E. coli was conducted on Eosin Methylene Blue Agar (EMBA), followed by identification on Sorbitol MacConkey Agar (SMAC). Then Molecular subtyping of E. coli O157:H7 genes was conducted using multiplex-PCR analysis. Data were analysed descriptively. The results of this study showed that 72 samples (85 %) among 85 samples were positive for E. coli and the rest of samples were positive for other types of bacteria. Sample isolation from swabs of hand was found 3 positive E. coli before work and 1 positive E. coli after work from 15 workers. The most potential water sources for E. coli contamination were the water in taps, and water puddle on the floor of cattle houses. Then, two of samples of E. coli isolated from rectal swab were confirmed as E. coli O157:H7 using PCR test, based on the presence of stx2 gene. In conclusion, the risk of presence of E. coli as zoonotic agents of E. coli O157:H7 in aceh cattle as well as from the farm workers and surrounding area are high. An appropriate control strategy is needed to apply in the aceh cattle farm to prevent from E. coli O157:H7 outbreak in the future.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01021.pdfaceh cattlepathogenworkerswatere. colizoonoses |
| spellingShingle | Ferasyi Teuku R. Abrar Mahdi Subianto Muhammad Afrianandra Cut Hambal Muhammad Razali Razali Ismail Ismail Nurliana Nurliana Rastina Rastina Sari Wahyu E. Safika Safika Vierman Vierman Mutia Nellita Barus Rina A. Yusmadi Yusmadi Rosa Teuku S. Ramadhan Rezky Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre E3S Web of Conferences aceh cattle pathogen workers water e. coli zoonoses |
| title | Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre |
| title_full | Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre |
| title_fullStr | Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre |
| title_full_unstemmed | Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre |
| title_short | Isolation, Identification, and Critical Points of Risk of Escherichia coli O157:H7 Contamination at Aceh Cattle Breeding Centre |
| title_sort | isolation identification and critical points of risk of escherichia coli o157 h7 contamination at aceh cattle breeding centre |
| topic | aceh cattle pathogen workers water e. coli zoonoses |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01021.pdf |
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