Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests
Zika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/3/654 |
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| author | Carlos Roberto Prudencio Vivaldo Gomes da Costa Leticia Barboza Rocha Hernan Hermes Monteiro Costa Diego José Belato Orts Felipe Rocha da Silva Santos Paula Rahal Nikolas Alexander Borsato Lino Pâmela Jóyce Previdelli da Conceição Cintia Bittar Rafael Rahal Guaragna Machado Edison Luiz Durigon João Pessoa Araujo Juliana Moutinho Polatto Miriam Aparecida da Silva Joyce Araújo de Oliveira Thais Mitsunari Lennon Ramos Pereira Robert Andreata-Santos Luís Carlos de Souza Ferreira Daniela Luz Roxane Maria Fontes Piazza |
| author_facet | Carlos Roberto Prudencio Vivaldo Gomes da Costa Leticia Barboza Rocha Hernan Hermes Monteiro Costa Diego José Belato Orts Felipe Rocha da Silva Santos Paula Rahal Nikolas Alexander Borsato Lino Pâmela Jóyce Previdelli da Conceição Cintia Bittar Rafael Rahal Guaragna Machado Edison Luiz Durigon João Pessoa Araujo Juliana Moutinho Polatto Miriam Aparecida da Silva Joyce Araújo de Oliveira Thais Mitsunari Lennon Ramos Pereira Robert Andreata-Santos Luís Carlos de Souza Ferreira Daniela Luz Roxane Maria Fontes Piazza |
| author_sort | Carlos Roberto Prudencio |
| collection | DOAJ |
| description | Zika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to the co-circulation of the dengue virus, which shares extensive sequence conservation of structural proteins leading to the generation of cross-reactive antibodies. In this study, we aimed to obtain tools for the development of improved serological tests for the detection of ZIKV infection. Polyclonal sera (pAb) and a monoclonal antibody (mAb 2F2) against a recombinant form of the ZIKV nonstructural protein 1 (NS1) allowed the identification of linear peptide epitopes of the NS1 protein. Based on these findings, six chemically synthesized peptides were tested both in dot blot and ELISA assays using convalescent sera collected from ZIKV-infected patients. Two of these peptides specifically detected the presence of ZIKV antibodies and proved to be candidates for the detection of ZIKV-infected subjects. The availability of these tools opens perspectives for the development of NS1-based serological tests with enhanced sensitivity regarding other flaviviruses. |
| first_indexed | 2024-03-11T05:46:50Z |
| format | Article |
| id | doaj.art-2f380db71d614c0dba67651962e6b110 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T05:46:50Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-2f380db71d614c0dba67651962e6b1102023-11-17T14:22:28ZengMDPI AGViruses1999-49152023-02-0115365410.3390/v15030654Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological TestsCarlos Roberto Prudencio0Vivaldo Gomes da Costa1Leticia Barboza Rocha2Hernan Hermes Monteiro Costa3Diego José Belato Orts4Felipe Rocha da Silva Santos5Paula Rahal6Nikolas Alexander Borsato Lino7Pâmela Jóyce Previdelli da Conceição8Cintia Bittar9Rafael Rahal Guaragna Machado10Edison Luiz Durigon11João Pessoa Araujo12Juliana Moutinho Polatto13Miriam Aparecida da Silva14Joyce Araújo de Oliveira15Thais Mitsunari16Lennon Ramos Pereira17Robert Andreata-Santos18Luís Carlos de Souza Ferreira19Daniela Luz20Roxane Maria Fontes Piazza21Laboratório de Imunobiotecnologia, Centro de Imunologia, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, São Paulo 01246-902, SP, BrazilInstituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, São Jose do Rio Preto 15054-000, SP, BrazilLaboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo 05503-900, SP, BrazilLaboratório de Imunobiotecnologia, Centro de Imunologia, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, São Paulo 01246-902, SP, BrazilLaboratório de Imunobiotecnologia, Centro de Imunologia, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, São Paulo 01246-902, SP, BrazilLaboratório de Imunobiotecnologia, Centro de Imunologia, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, São Paulo 01246-902, SP, BrazilInstituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, São Jose do Rio Preto 15054-000, SP, BrazilInstituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, São Jose do Rio Preto 15054-000, SP, BrazilInstituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, São Jose do Rio Preto 15054-000, SP, BrazilInstituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, São Jose do Rio Preto 15054-000, SP, BrazilDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, SP, BrazilDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, SP, BrazilInstituto de Biotecnologia, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu 18607-440, SP, BrazilLaboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo 05503-900, SP, BrazilLaboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo 05503-900, SP, BrazilLaboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo 05503-900, SP, BrazilLaboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo 05503-900, SP, BrazilDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, SP, BrazilDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, SP, BrazilDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, SP, BrazilLaboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo 05503-900, SP, BrazilLaboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo 05503-900, SP, BrazilZika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to the co-circulation of the dengue virus, which shares extensive sequence conservation of structural proteins leading to the generation of cross-reactive antibodies. In this study, we aimed to obtain tools for the development of improved serological tests for the detection of ZIKV infection. Polyclonal sera (pAb) and a monoclonal antibody (mAb 2F2) against a recombinant form of the ZIKV nonstructural protein 1 (NS1) allowed the identification of linear peptide epitopes of the NS1 protein. Based on these findings, six chemically synthesized peptides were tested both in dot blot and ELISA assays using convalescent sera collected from ZIKV-infected patients. Two of these peptides specifically detected the presence of ZIKV antibodies and proved to be candidates for the detection of ZIKV-infected subjects. The availability of these tools opens perspectives for the development of NS1-based serological tests with enhanced sensitivity regarding other flaviviruses.https://www.mdpi.com/1999-4915/15/3/654Zika virusNS1 proteinmonoclonal antibodyepitopespeptidesimmunoassays |
| spellingShingle | Carlos Roberto Prudencio Vivaldo Gomes da Costa Leticia Barboza Rocha Hernan Hermes Monteiro Costa Diego José Belato Orts Felipe Rocha da Silva Santos Paula Rahal Nikolas Alexander Borsato Lino Pâmela Jóyce Previdelli da Conceição Cintia Bittar Rafael Rahal Guaragna Machado Edison Luiz Durigon João Pessoa Araujo Juliana Moutinho Polatto Miriam Aparecida da Silva Joyce Araújo de Oliveira Thais Mitsunari Lennon Ramos Pereira Robert Andreata-Santos Luís Carlos de Souza Ferreira Daniela Luz Roxane Maria Fontes Piazza Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests Viruses Zika virus NS1 protein monoclonal antibody epitopes peptides immunoassays |
| title | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
| title_full | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
| title_fullStr | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
| title_full_unstemmed | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
| title_short | Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests |
| title_sort | identification of zika virus ns1 derived peptides with potential applications in serological tests |
| topic | Zika virus NS1 protein monoclonal antibody epitopes peptides immunoassays |
| url | https://www.mdpi.com/1999-4915/15/3/654 |
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