Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete <i>Streptomyces cyaneofuscatus</i> M-169
The potent antimicrobial extract of a culture of the marine derived actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 was fractionated by reversed phase flash chromatography and preparative HPLC to yield the new Gram-positive antibiotic, anthracimycin B (<b>1</b>), togeth...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-10-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/16/11/406 |
| _version_ | 1798039844123836416 |
|---|---|
| author | Víctor Rodríguez Jesús Martín Aida Sarmiento-Vizcaíno Mercedes de la Cruz Luis A. García Gloria Blanco Fernando Reyes |
| author_facet | Víctor Rodríguez Jesús Martín Aida Sarmiento-Vizcaíno Mercedes de la Cruz Luis A. García Gloria Blanco Fernando Reyes |
| author_sort | Víctor Rodríguez |
| collection | DOAJ |
| description | The potent antimicrobial extract of a culture of the marine derived actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 was fractionated by reversed phase flash chromatography and preparative HPLC to yield the new Gram-positive antibiotic, anthracimycin B (<b>1</b>), together with its congener, anthracimycin (<b>2</b>). The structure of the new compound was established by analysis of its ESI-TOF MS and 1D and 2D NMR spectra, and comparison with data published for anthracimycin and anthracimycin BII-2619 (<b>3</b>). Notably, anthracimycin seemed to be the major and almost unique component of the extract detected by HPLC-UV-MS, making our <i>S. cyanofuscatus</i> strain an excellent candidate for further biosynthetic studies of this potent antibiotic. |
| first_indexed | 2024-04-11T21:59:17Z |
| format | Article |
| id | doaj.art-2f3d18b9b8e14cf88f8ba0a3568cbc37 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T21:59:17Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-2f3d18b9b8e14cf88f8ba0a3568cbc372022-12-22T04:01:00ZengMDPI AGMarine Drugs1660-33972018-10-01161140610.3390/md16110406md16110406Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete <i>Streptomyces cyaneofuscatus</i> M-169Víctor Rodríguez0Jesús Martín1Aida Sarmiento-Vizcaíno2Mercedes de la Cruz3Luis A. García4Gloria Blanco5Fernando Reyes6Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, SpainDepartamento de Biología Funcional, Área de Microbiología, and Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, SpainDepartamento de Ingeniería Química y Tecnología del Medio Ambiente, Área de Ingeniería Química, Universidad de Oviedo, 33006 Oviedo, SpainDepartamento de Biología Funcional, Área de Microbiología, and Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, SpainFundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, SpainThe potent antimicrobial extract of a culture of the marine derived actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 was fractionated by reversed phase flash chromatography and preparative HPLC to yield the new Gram-positive antibiotic, anthracimycin B (<b>1</b>), together with its congener, anthracimycin (<b>2</b>). The structure of the new compound was established by analysis of its ESI-TOF MS and 1D and 2D NMR spectra, and comparison with data published for anthracimycin and anthracimycin BII-2619 (<b>3</b>). Notably, anthracimycin seemed to be the major and almost unique component of the extract detected by HPLC-UV-MS, making our <i>S. cyanofuscatus</i> strain an excellent candidate for further biosynthetic studies of this potent antibiotic.https://www.mdpi.com/1660-3397/16/11/406anthracimycin<i>Streptomyces cyaneofuscatus</i>deep-sea actinobacteriaCantabrian Sea |
| spellingShingle | Víctor Rodríguez Jesús Martín Aida Sarmiento-Vizcaíno Mercedes de la Cruz Luis A. García Gloria Blanco Fernando Reyes Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 Marine Drugs anthracimycin <i>Streptomyces cyaneofuscatus</i> deep-sea actinobacteria Cantabrian Sea |
| title | Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 |
| title_full | Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 |
| title_fullStr | Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 |
| title_full_unstemmed | Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 |
| title_short | Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete <i>Streptomyces cyaneofuscatus</i> M-169 |
| title_sort | anthracimycin b a potent antibiotic against gram positive bacteria isolated from cultures of the deep sea actinomycete i streptomyces cyaneofuscatus i m 169 |
| topic | anthracimycin <i>Streptomyces cyaneofuscatus</i> deep-sea actinobacteria Cantabrian Sea |
| url | https://www.mdpi.com/1660-3397/16/11/406 |
| work_keys_str_mv | AT victorrodriguez anthracimycinbapotentantibioticagainstgrampositivebacteriaisolatedfromculturesofthedeepseaactinomyceteistreptomycescyaneofuscatusim169 AT jesusmartin anthracimycinbapotentantibioticagainstgrampositivebacteriaisolatedfromculturesofthedeepseaactinomyceteistreptomycescyaneofuscatusim169 AT aidasarmientovizcaino anthracimycinbapotentantibioticagainstgrampositivebacteriaisolatedfromculturesofthedeepseaactinomyceteistreptomycescyaneofuscatusim169 AT mercedesdelacruz anthracimycinbapotentantibioticagainstgrampositivebacteriaisolatedfromculturesofthedeepseaactinomyceteistreptomycescyaneofuscatusim169 AT luisagarcia anthracimycinbapotentantibioticagainstgrampositivebacteriaisolatedfromculturesofthedeepseaactinomyceteistreptomycescyaneofuscatusim169 AT gloriablanco anthracimycinbapotentantibioticagainstgrampositivebacteriaisolatedfromculturesofthedeepseaactinomyceteistreptomycescyaneofuscatusim169 AT fernandoreyes anthracimycinbapotentantibioticagainstgrampositivebacteriaisolatedfromculturesofthedeepseaactinomyceteistreptomycescyaneofuscatusim169 |