Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to Oxidation
Although the functions and structural roles of sterols have been the subject of numerous studies, the reasons for the diversity of sterols in the different eukaryotic kingdoms remain unclear. It is thought that the specificity of sterols is linked to unidentified supplementary functions that could e...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/7/1024 |
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| author | Sebastien Dupont Paul Fleurat-Lessard Richtier Gonçalves Cruz Céline Lafarge Cédric Grangeteau Fairouz Yahou Patricia Gerbeau-Pissot Odonírio Abrahão Júnior Patrick Gervais Françoise Simon-Plas Philippe Cayot Laurent Beney |
| author_facet | Sebastien Dupont Paul Fleurat-Lessard Richtier Gonçalves Cruz Céline Lafarge Cédric Grangeteau Fairouz Yahou Patricia Gerbeau-Pissot Odonírio Abrahão Júnior Patrick Gervais Françoise Simon-Plas Philippe Cayot Laurent Beney |
| author_sort | Sebastien Dupont |
| collection | DOAJ |
| description | Although the functions and structural roles of sterols have been the subject of numerous studies, the reasons for the diversity of sterols in the different eukaryotic kingdoms remain unclear. It is thought that the specificity of sterols is linked to unidentified supplementary functions that could enable organisms to be better adapted to their environment. Ergosterol is accumulated by late branching fungi that encounter oxidative perturbations in their interfacial habitats. Here, we investigated the antioxidant properties of ergosterol using in vivo, in vitro, and in silico approaches. The results showed that ergosterol is involved in yeast resistance to tert-butyl hydroperoxide and protects lipids against oxidation in liposomes. A computational study based on quantum chemistry revealed that this protection could be related to its antioxidant properties operating through an electron transfer followed by a proton transfer mechanism. This study demonstrates the antioxidant role of ergosterol and proposes knowledge elements to explain the specific accumulation of this sterol in late branching fungi. Ergosterol, as a natural antioxidant molecule, could also play a role in the incompletely understood beneficial effects of some mushrooms on health. |
| first_indexed | 2024-03-10T10:03:21Z |
| format | Article |
| id | doaj.art-2f3e37f173f941bc90a1057b962e7537 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T10:03:21Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-2f3e37f173f941bc90a1057b962e75372023-11-22T01:44:07ZengMDPI AGAntioxidants2076-39212021-06-01107102410.3390/antiox10071024Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to OxidationSebastien Dupont0Paul Fleurat-Lessard1Richtier Gonçalves Cruz2Céline Lafarge3Cédric Grangeteau4Fairouz Yahou5Patricia Gerbeau-Pissot6Odonírio Abrahão Júnior7Patrick Gervais8Françoise Simon-Plas9Philippe Cayot10Laurent Beney11UMR Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, 21000 Dijon, FranceInstitut de Chimie Moléculaire de l’Université de Bourgogne, University Bourgogne Franche-Comté, ICMUB—UMR CNRS 6302, CEDEX, 21078 Dijon, FranceUMR Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, 21000 Dijon, FranceUMR Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, 21000 Dijon, FranceUMR Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, 21000 Dijon, FranceUMR Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, 21000 Dijon, FranceUMR1347 Agroécologie, ERL 6300 CNRS, INRA, CEDEX, 21065 Dijon, FranceInstituto de Ciências Biológicas e Naturais, Universidade Federal do Triangulo Mineiro, Uberaba 38025-180, BrazilUMR Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, 21000 Dijon, FranceUMR1347 Agroécologie, ERL 6300 CNRS, INRA, CEDEX, 21065 Dijon, FranceUMR Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, 21000 Dijon, FranceUMR Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, 21000 Dijon, FranceAlthough the functions and structural roles of sterols have been the subject of numerous studies, the reasons for the diversity of sterols in the different eukaryotic kingdoms remain unclear. It is thought that the specificity of sterols is linked to unidentified supplementary functions that could enable organisms to be better adapted to their environment. Ergosterol is accumulated by late branching fungi that encounter oxidative perturbations in their interfacial habitats. Here, we investigated the antioxidant properties of ergosterol using in vivo, in vitro, and in silico approaches. The results showed that ergosterol is involved in yeast resistance to tert-butyl hydroperoxide and protects lipids against oxidation in liposomes. A computational study based on quantum chemistry revealed that this protection could be related to its antioxidant properties operating through an electron transfer followed by a proton transfer mechanism. This study demonstrates the antioxidant role of ergosterol and proposes knowledge elements to explain the specific accumulation of this sterol in late branching fungi. Ergosterol, as a natural antioxidant molecule, could also play a role in the incompletely understood beneficial effects of some mushrooms on health.https://www.mdpi.com/2076-3921/10/7/1024steroloxidationantioxidantlipidsyeastplasma membrane |
| spellingShingle | Sebastien Dupont Paul Fleurat-Lessard Richtier Gonçalves Cruz Céline Lafarge Cédric Grangeteau Fairouz Yahou Patricia Gerbeau-Pissot Odonírio Abrahão Júnior Patrick Gervais Françoise Simon-Plas Philippe Cayot Laurent Beney Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to Oxidation Antioxidants sterol oxidation antioxidant lipids yeast plasma membrane |
| title | Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to Oxidation |
| title_full | Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to Oxidation |
| title_fullStr | Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to Oxidation |
| title_full_unstemmed | Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to Oxidation |
| title_short | Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to Oxidation |
| title_sort | antioxidant properties of ergosterol and its role in yeast resistance to oxidation |
| topic | sterol oxidation antioxidant lipids yeast plasma membrane |
| url | https://www.mdpi.com/2076-3921/10/7/1024 |
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