Effects of Extracellular Vesicles from Osteogenic Differentiated Human BMSCs on Osteogenic and Adipogenic Differentiation Capacity of Naïve Human BMSCs
Osteoporosis, or steroid-induced osteonecrosis of the hip, is accompanied by increased bone marrow adipogenesis. Such a disorder of adipogenic/osteogenic differentiation, affecting bone-marrow-derived mesenchymal stem cells (BMSCs), contributes to bone loss during aging. Here, we investigated the ef...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/16/2491 |
| _version_ | 1797446468523524096 |
|---|---|
| author | Chenglong Wang Sabine Stöckl Shushan Li Marietta Herrmann Christoph Lukas Yvonne Reinders Albert Sickmann Susanne Grässel |
| author_facet | Chenglong Wang Sabine Stöckl Shushan Li Marietta Herrmann Christoph Lukas Yvonne Reinders Albert Sickmann Susanne Grässel |
| author_sort | Chenglong Wang |
| collection | DOAJ |
| description | Osteoporosis, or steroid-induced osteonecrosis of the hip, is accompanied by increased bone marrow adipogenesis. Such a disorder of adipogenic/osteogenic differentiation, affecting bone-marrow-derived mesenchymal stem cells (BMSCs), contributes to bone loss during aging. Here, we investigated the effects of extracellular vesicles (EVs) isolated from human (h)BMSCs during different stages of osteogenic differentiation on the osteogenic and adipogenic differentiation capacity of naïve (undifferentiated) hBMSCs. We observed that all EV groups increased viability and proliferation capacity and suppressed the apoptosis of naïve hBMSCs. In particular, EVs derived from hBMSCs at late-stage osteogenic differentiation promoted the osteogenic potential of naïve hBMSCs more effectively than EVs derived from naïve hBMSCs (naïve EVs), as indicated by the increased gene expression of COL1A1 and OPN. In contrast, the adipogenic differentiation capacity of naïve hBMSCs was inhibited by treatment with EVs from osteogenic differentiated hBMSCs. Proteomic analysis revealed that osteogenic EVs and naïve EVs contained distinct protein profiles, with pro-osteogenic and anti-adipogenic proteins encapsulated in osteogenic EVs. We speculate that osteogenic EVs could serve as an intercellular communication system between bone- and bone-marrow adipose tissue, for transporting osteogenic factors and thus favoring pro-osteogenic processes. Our data may support the theory of an endocrine circuit with the skeleton functioning as a ductless gland. |
| first_indexed | 2024-03-09T13:41:07Z |
| format | Article |
| id | doaj.art-2f3f6a50af514076968a12b723859791 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T13:41:07Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2f3f6a50af514076968a12b7238597912023-11-30T21:06:24ZengMDPI AGCells2073-44092022-08-011116249110.3390/cells11162491Effects of Extracellular Vesicles from Osteogenic Differentiated Human BMSCs on Osteogenic and Adipogenic Differentiation Capacity of Naïve Human BMSCsChenglong Wang0Sabine Stöckl1Shushan Li2Marietta Herrmann3Christoph Lukas4Yvonne Reinders5Albert Sickmann6Susanne Grässel7Department of Orthopedic Surgery, Experimental Orthopedics, Centre for Medical Biotechnology (ZMB), University of Regensburg, 93053 Regensburg, GermanyDepartment of Orthopedic Surgery, Experimental Orthopedics, Centre for Medical Biotechnology (ZMB), University of Regensburg, 93053 Regensburg, GermanyDepartment of Orthopedic Surgery, Experimental Orthopedics, Centre for Medical Biotechnology (ZMB), University of Regensburg, 93053 Regensburg, GermanyIZKF Group Tissue Reg. in Musculoskeletal Dis., University Hospital & Bernhard-Heine-Centrum for Locomotion Res., University of Würzburg, 97070 Würzburg, GermanyDepartment of Orthopedic Surgery, Experimental Orthopedics, Centre for Medical Biotechnology (ZMB), University of Regensburg, 93053 Regensburg, GermanyLeibniz-Institut für Analytische Wissenschaften–ISAS–e.V., Bunsen-Kirchhoff-Straße 11, 44139 Dortmund, GermanyLeibniz-Institut für Analytische Wissenschaften–ISAS–e.V., Bunsen-Kirchhoff-Straße 11, 44139 Dortmund, GermanyDepartment of Orthopedic Surgery, Experimental Orthopedics, Centre for Medical Biotechnology (ZMB), University of Regensburg, 93053 Regensburg, GermanyOsteoporosis, or steroid-induced osteonecrosis of the hip, is accompanied by increased bone marrow adipogenesis. Such a disorder of adipogenic/osteogenic differentiation, affecting bone-marrow-derived mesenchymal stem cells (BMSCs), contributes to bone loss during aging. Here, we investigated the effects of extracellular vesicles (EVs) isolated from human (h)BMSCs during different stages of osteogenic differentiation on the osteogenic and adipogenic differentiation capacity of naïve (undifferentiated) hBMSCs. We observed that all EV groups increased viability and proliferation capacity and suppressed the apoptosis of naïve hBMSCs. In particular, EVs derived from hBMSCs at late-stage osteogenic differentiation promoted the osteogenic potential of naïve hBMSCs more effectively than EVs derived from naïve hBMSCs (naïve EVs), as indicated by the increased gene expression of COL1A1 and OPN. In contrast, the adipogenic differentiation capacity of naïve hBMSCs was inhibited by treatment with EVs from osteogenic differentiated hBMSCs. Proteomic analysis revealed that osteogenic EVs and naïve EVs contained distinct protein profiles, with pro-osteogenic and anti-adipogenic proteins encapsulated in osteogenic EVs. We speculate that osteogenic EVs could serve as an intercellular communication system between bone- and bone-marrow adipose tissue, for transporting osteogenic factors and thus favoring pro-osteogenic processes. Our data may support the theory of an endocrine circuit with the skeleton functioning as a ductless gland.https://www.mdpi.com/2073-4409/11/16/2491extracellular vesiclesmesenchymal stem cellsosteogenic potentialosteogenic differentiationadipogenic differentiationECM remodeling |
| spellingShingle | Chenglong Wang Sabine Stöckl Shushan Li Marietta Herrmann Christoph Lukas Yvonne Reinders Albert Sickmann Susanne Grässel Effects of Extracellular Vesicles from Osteogenic Differentiated Human BMSCs on Osteogenic and Adipogenic Differentiation Capacity of Naïve Human BMSCs Cells extracellular vesicles mesenchymal stem cells osteogenic potential osteogenic differentiation adipogenic differentiation ECM remodeling |
| title | Effects of Extracellular Vesicles from Osteogenic Differentiated Human BMSCs on Osteogenic and Adipogenic Differentiation Capacity of Naïve Human BMSCs |
| title_full | Effects of Extracellular Vesicles from Osteogenic Differentiated Human BMSCs on Osteogenic and Adipogenic Differentiation Capacity of Naïve Human BMSCs |
| title_fullStr | Effects of Extracellular Vesicles from Osteogenic Differentiated Human BMSCs on Osteogenic and Adipogenic Differentiation Capacity of Naïve Human BMSCs |
| title_full_unstemmed | Effects of Extracellular Vesicles from Osteogenic Differentiated Human BMSCs on Osteogenic and Adipogenic Differentiation Capacity of Naïve Human BMSCs |
| title_short | Effects of Extracellular Vesicles from Osteogenic Differentiated Human BMSCs on Osteogenic and Adipogenic Differentiation Capacity of Naïve Human BMSCs |
| title_sort | effects of extracellular vesicles from osteogenic differentiated human bmscs on osteogenic and adipogenic differentiation capacity of naive human bmscs |
| topic | extracellular vesicles mesenchymal stem cells osteogenic potential osteogenic differentiation adipogenic differentiation ECM remodeling |
| url | https://www.mdpi.com/2073-4409/11/16/2491 |
| work_keys_str_mv | AT chenglongwang effectsofextracellularvesiclesfromosteogenicdifferentiatedhumanbmscsonosteogenicandadipogenicdifferentiationcapacityofnaivehumanbmscs AT sabinestockl effectsofextracellularvesiclesfromosteogenicdifferentiatedhumanbmscsonosteogenicandadipogenicdifferentiationcapacityofnaivehumanbmscs AT shushanli effectsofextracellularvesiclesfromosteogenicdifferentiatedhumanbmscsonosteogenicandadipogenicdifferentiationcapacityofnaivehumanbmscs AT mariettaherrmann effectsofextracellularvesiclesfromosteogenicdifferentiatedhumanbmscsonosteogenicandadipogenicdifferentiationcapacityofnaivehumanbmscs AT christophlukas effectsofextracellularvesiclesfromosteogenicdifferentiatedhumanbmscsonosteogenicandadipogenicdifferentiationcapacityofnaivehumanbmscs AT yvonnereinders effectsofextracellularvesiclesfromosteogenicdifferentiatedhumanbmscsonosteogenicandadipogenicdifferentiationcapacityofnaivehumanbmscs AT albertsickmann effectsofextracellularvesiclesfromosteogenicdifferentiatedhumanbmscsonosteogenicandadipogenicdifferentiationcapacityofnaivehumanbmscs AT susannegrassel effectsofextracellularvesiclesfromosteogenicdifferentiatedhumanbmscsonosteogenicandadipogenicdifferentiationcapacityofnaivehumanbmscs |