Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation
Summary: This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which ha...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672100589X |
| _version_ | 1818873794594340864 |
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| author | Laura G. Rico Roser Salvia Michael D. Ward Jolene A. Bradford Jordi Petriz |
| author_facet | Laura G. Rico Roser Salvia Michael D. Ward Jolene A. Bradford Jordi Petriz |
| author_sort | Laura G. Rico |
| collection | DOAJ |
| description | Summary: This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which have been successfully used to identify healthy and pathologically rare cells. The greatest advantage of these approaches is that they minimize the unwanted effect caused by sample preparation, allowing for improved study of live cells at the point of analysis.For complete details on the use and execution of this protocol, please refer to Petriz et al. (2018). |
| first_indexed | 2024-12-19T13:00:22Z |
| format | Article |
| id | doaj.art-2f454dda604e4ae5b90cdc008b5d3de4 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-19T13:00:22Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-2f454dda604e4ae5b90cdc008b5d3de42022-12-21T20:20:15ZengElsevierSTAR Protocols2666-16672021-12-0124100883Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbationLaura G. Rico0Roser Salvia1Michael D. Ward2Jolene A. Bradford3Jordi Petriz4Functional Cytomics Lab, Josep Carreras Leukaemia Research Institute, ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Barcelona, SpainFunctional Cytomics Lab, Josep Carreras Leukaemia Research Institute, ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Barcelona, SpainThermo Fisher Scientific, Eugene, OR, USA; Corresponding authorThermo Fisher Scientific, Eugene, OR, USAFunctional Cytomics Lab, Josep Carreras Leukaemia Research Institute, ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Barcelona, Spain; Corresponding authorSummary: This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which have been successfully used to identify healthy and pathologically rare cells. The greatest advantage of these approaches is that they minimize the unwanted effect caused by sample preparation, allowing for improved study of live cells at the point of analysis.For complete details on the use and execution of this protocol, please refer to Petriz et al. (2018).http://www.sciencedirect.com/science/article/pii/S266616672100589XFlow Cytometry/Mass CytometryCancerHealth SciencesClinical ProtocolImmunologyStem Cells |
| spellingShingle | Laura G. Rico Roser Salvia Michael D. Ward Jolene A. Bradford Jordi Petriz Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation STAR Protocols Flow Cytometry/Mass Cytometry Cancer Health Sciences Clinical Protocol Immunology Stem Cells |
| title | Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation |
| title_full | Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation |
| title_fullStr | Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation |
| title_full_unstemmed | Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation |
| title_short | Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation |
| title_sort | flow cytometry based protocols for human blood marrow immunophenotyping with minimal sample perturbation |
| topic | Flow Cytometry/Mass Cytometry Cancer Health Sciences Clinical Protocol Immunology Stem Cells |
| url | http://www.sciencedirect.com/science/article/pii/S266616672100589X |
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