Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation

Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation

Summary: This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which ha...

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Bibliographic Details
Main Authors:	Laura G. Rico, Roser Salvia, Michael D. Ward, Jolene A. Bradford, Jordi Petriz
Format:	Article
Language:	English
Published:	Elsevier 2021-12-01
Series:	STAR Protocols
Subjects:	Flow Cytometry/Mass Cytometry Cancer Health Sciences Clinical Protocol Immunology Stem Cells
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672100589X

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