Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed
Rapeseed (Brassica napus) is one of the most important oil crops worldwide; however, drought seriously curtails its growth and productivity. Identifying drought-tolerant germplasm is an efficient strategy for addressing water shortages. Here, we phenotyped a panel of 264 B. napus accessions at full-...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-03-01
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| Series: | Oil Crop Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2096242821000026 |
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| author | Ali Shahzad Minchao Qian Bangyang Sun Umer Mahmood Shengting Li Yonghai Fan Wei Chang Lishi Dai Hong Zhu Jiana Li Cunmin Qu Kun Lu |
| author_facet | Ali Shahzad Minchao Qian Bangyang Sun Umer Mahmood Shengting Li Yonghai Fan Wei Chang Lishi Dai Hong Zhu Jiana Li Cunmin Qu Kun Lu |
| author_sort | Ali Shahzad |
| collection | DOAJ |
| description | Rapeseed (Brassica napus) is one of the most important oil crops worldwide; however, drought seriously curtails its growth and productivity. Identifying drought-tolerant germplasm is an efficient strategy for addressing water shortages. Here, we phenotyped a panel of 264 B. napus accessions at full-bloom stage using water loss ratio (WLR) as drought-tolerant index. It identified 8 low-WLR and 6 high-WLR accessions, regarded as drought-tolerant and drought-sensitive, respectively. Comparing with drought-sensitive accessions at the seedling stage, drought-tolerant accessions had shown better performance in maintaining fresh and dry weights, and performed the higher expression of drought-induced marker genes under drought stress. Subsequently, a total of 139 SNPs (single nucleotide polymorphisms) were identified associated with the WLR using a genome-wide association study (GWAS) among 264 B. napus accessions, with the largest number SNPs at chromosome A10, and 13 SNPs significantly were associated with the WLR (–log10 (p-value) > 6). Furthermore, 4 putative candidate genes (BnaC09.RPS6, BnaC09.MATE, BnaA10.PPD5 and BnaC09.Histone) were screened involving in drought tolerance in B. napus. Together, our results highlight the WLR's importance in drought tolerance and establish the foundation for improving WLR-associated drought tolerance in rapeseed. |
| first_indexed | 2024-12-19T12:57:59Z |
| format | Article |
| id | doaj.art-2f47aaba3d52430d99364e60e7bb3713 |
| institution | Directory Open Access Journal |
| issn | 2096-2428 |
| language | English |
| last_indexed | 2024-12-19T12:57:59Z |
| publishDate | 2021-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Oil Crop Science |
| spelling | doaj.art-2f47aaba3d52430d99364e60e7bb37132022-12-21T20:20:21ZengKeAi Communications Co., Ltd.Oil Crop Science2096-24282021-03-01611222Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseedAli Shahzad0Minchao Qian1Bangyang Sun2Umer Mahmood3Shengting Li4Yonghai Fan5Wei Chang6Lishi Dai7Hong Zhu8Jiana Li9Cunmin Qu10Kun Lu11College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, 400715, China; Corresponding author.College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, 400715, China; College of Life Sciences, Yangtze University, Jingzhou, 434025, Hubei, China; Corresponding author.Rapeseed (Brassica napus) is one of the most important oil crops worldwide; however, drought seriously curtails its growth and productivity. Identifying drought-tolerant germplasm is an efficient strategy for addressing water shortages. Here, we phenotyped a panel of 264 B. napus accessions at full-bloom stage using water loss ratio (WLR) as drought-tolerant index. It identified 8 low-WLR and 6 high-WLR accessions, regarded as drought-tolerant and drought-sensitive, respectively. Comparing with drought-sensitive accessions at the seedling stage, drought-tolerant accessions had shown better performance in maintaining fresh and dry weights, and performed the higher expression of drought-induced marker genes under drought stress. Subsequently, a total of 139 SNPs (single nucleotide polymorphisms) were identified associated with the WLR using a genome-wide association study (GWAS) among 264 B. napus accessions, with the largest number SNPs at chromosome A10, and 13 SNPs significantly were associated with the WLR (–log10 (p-value) > 6). Furthermore, 4 putative candidate genes (BnaC09.RPS6, BnaC09.MATE, BnaA10.PPD5 and BnaC09.Histone) were screened involving in drought tolerance in B. napus. Together, our results highlight the WLR's importance in drought tolerance and establish the foundation for improving WLR-associated drought tolerance in rapeseed.http://www.sciencedirect.com/science/article/pii/S2096242821000026GWASWater loss ratioDroughtSNPRapeseed |
| spellingShingle | Ali Shahzad Minchao Qian Bangyang Sun Umer Mahmood Shengting Li Yonghai Fan Wei Chang Lishi Dai Hong Zhu Jiana Li Cunmin Qu Kun Lu Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed Oil Crop Science GWAS Water loss ratio Drought SNP Rapeseed |
| title | Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed |
| title_full | Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed |
| title_fullStr | Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed |
| title_full_unstemmed | Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed |
| title_short | Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed |
| title_sort | genome wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed |
| topic | GWAS Water loss ratio Drought SNP Rapeseed |
| url | http://www.sciencedirect.com/science/article/pii/S2096242821000026 |
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