<i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment
Canine mammary gland tumors (CMTs) are one of the most prevalent cancers in dogs and a good model for human breast cancer (BC), however gene expression analysis of CMTs is scarce. Although divergence of genes expression has been found in BC of different human races, no such research of different dog...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/8/10/212 |
| _version_ | 1797512980081934336 |
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| author | Simona Sakalauskaitė Violeta Šaltenienė Darja Nikitina Rasa Ugenskienė Vita Riškevičienė Birutė Karvelienė Nomeda Juodžiukynienė |
| author_facet | Simona Sakalauskaitė Violeta Šaltenienė Darja Nikitina Rasa Ugenskienė Vita Riškevičienė Birutė Karvelienė Nomeda Juodžiukynienė |
| author_sort | Simona Sakalauskaitė |
| collection | DOAJ |
| description | Canine mammary gland tumors (CMTs) are one of the most prevalent cancers in dogs and a good model for human breast cancer (BC), however gene expression analysis of CMTs is scarce. Although divergence of genes expression has been found in BC of different human races, no such research of different dog’s breeds has been done. The purpose of this study was to investigate expression of the <i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> genes of canine mammary carcinomas, compare the expression levels with clinicopathological parameters and analyze expression disparities between different breeds. Carcinomas and adjacent tissues were collected from female dogs to perform routine histopathology, immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR). We found that <i>VEGF-B</i> and <i>EGFR</i> genes were overexpressed in the mammary gland carcinomas compared to adjacent tissue. <i>VEGF-B</i> gene expression had associations with different parameters (tumor size, grade, and absence of metastasis). Furthermore, differences in <i>VEGF-B</i>, <i>FLT1</i>, <i>ERBB2</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> genes expression have been found in different breed dogs (German Shepherd, Yorkshire Terrier) and mixed-breed dogs indicating that a dog’s breed could determine a molecular difference, outcome of cancer and should be accounted as a confounding factor in the future gene expression research. |
| first_indexed | 2024-03-10T06:09:14Z |
| format | Article |
| id | doaj.art-2f50a2f3cb3a4ca89a905519073b4d0f |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T06:09:14Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-2f50a2f3cb3a4ca89a905519073b4d0f2023-11-22T20:17:07ZengMDPI AGVeterinary Sciences2306-73812021-09-0181021210.3390/vetsci8100212<i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed AssessmentSimona Sakalauskaitė0Violeta Šaltenienė1Darja Nikitina2Rasa Ugenskienė3Vita Riškevičienė4Birutė Karvelienė5Nomeda Juodžiukynienė6Department of Veterinary Pathobiology, Faculty of Veterinary Medicine, Veterinary Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaLaboratory of Clinical and Molecular Gastroenterology, Institute for Digestive Research, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaLaboratory of Clinical and Molecular Gastroenterology, Institute for Digestive Research, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Genetics and Molecular Medicine, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Veterinary Pathobiology, Faculty of Veterinary Medicine, Veterinary Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDr. L Kraučeliūnas Small Animal Clinic, Faculty of Veterinary Medicine, Veterinary Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaDepartment of Veterinary Pathobiology, Faculty of Veterinary Medicine, Veterinary Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, LithuaniaCanine mammary gland tumors (CMTs) are one of the most prevalent cancers in dogs and a good model for human breast cancer (BC), however gene expression analysis of CMTs is scarce. Although divergence of genes expression has been found in BC of different human races, no such research of different dog’s breeds has been done. The purpose of this study was to investigate expression of the <i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> genes of canine mammary carcinomas, compare the expression levels with clinicopathological parameters and analyze expression disparities between different breeds. Carcinomas and adjacent tissues were collected from female dogs to perform routine histopathology, immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR). We found that <i>VEGF-B</i> and <i>EGFR</i> genes were overexpressed in the mammary gland carcinomas compared to adjacent tissue. <i>VEGF-B</i> gene expression had associations with different parameters (tumor size, grade, and absence of metastasis). Furthermore, differences in <i>VEGF-B</i>, <i>FLT1</i>, <i>ERBB2</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> genes expression have been found in different breed dogs (German Shepherd, Yorkshire Terrier) and mixed-breed dogs indicating that a dog’s breed could determine a molecular difference, outcome of cancer and should be accounted as a confounding factor in the future gene expression research.https://www.mdpi.com/2306-7381/8/10/212canine mammary carcinomabreedgene expression<i>VEGF-B</i><i>EGFR</i> |
| spellingShingle | Simona Sakalauskaitė Violeta Šaltenienė Darja Nikitina Rasa Ugenskienė Vita Riškevičienė Birutė Karvelienė Nomeda Juodžiukynienė <i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment Veterinary Sciences canine mammary carcinoma breed gene expression <i>VEGF-B</i> <i>EGFR</i> |
| title | <i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment |
| title_full | <i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment |
| title_fullStr | <i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment |
| title_full_unstemmed | <i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment |
| title_short | <i>VEGF-B</i>, <i>VEGF-A</i>, <i>FLT-1</i>, <i>KDR</i>, <i>ERBB2</i>, <i>EGFR</i>, <i>GRB2</i>, <i>RAC1</i>, <i>CDH1</i> and <i>HYAL-1</i> Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment |
| title_sort | i vegf b i i vegf a i i flt 1 i i kdr i i erbb2 i i egfr i i grb2 i i rac1 i i cdh1 i and i hyal 1 i genes expression analysis in canine mammary gland tumors and the association with tumor clinicopathological parameters and dog breed assessment |
| topic | canine mammary carcinoma breed gene expression <i>VEGF-B</i> <i>EGFR</i> |
| url | https://www.mdpi.com/2306-7381/8/10/212 |
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