A Purified Anthraquinone-Glycoside Preparation From Rhubarb Ameliorates Type 2 Diabetes Mellitus by Modulating the Gut Microbiota and Reducing Inflammation
Rheum palmatum L. is widely used in traditional Chinese medicine for the treatment of constipation. Here, the therapeutic effects and underlying mechanisms of purified anthraquinone-glycoside preparation from rhubarb (RAGP) on the type 2 diabetes mellitus (T2DM) rats were investigated. After 6 weeks...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01423/full |
| _version_ | 1831840649550233600 |
|---|---|
| author | Hong-Xin Cui Hong-Xin Cui Ling-Shuai Zhang Yang Luo Ke Yuan Zhi-Yong Huang Ying Guo |
| author_facet | Hong-Xin Cui Hong-Xin Cui Ling-Shuai Zhang Yang Luo Ke Yuan Zhi-Yong Huang Ying Guo |
| author_sort | Hong-Xin Cui |
| collection | DOAJ |
| description | Rheum palmatum L. is widely used in traditional Chinese medicine for the treatment of constipation. Here, the therapeutic effects and underlying mechanisms of purified anthraquinone-glycoside preparation from rhubarb (RAGP) on the type 2 diabetes mellitus (T2DM) rats were investigated. After 6 weeks of metformin and RAGP treatment, the weight returned to normal. Fasting blood glucose (FBG), glycated serum protein (GSP), insulin concentration and HOMA-IR index had significantly decreased, and glucagon-like peptide-1 (GLP-1) concentrations had increased. Histological abnormalities in the pancreas and ileum had improved. These effects were associated with enhanced intestinal integrity, thereby reducing the absorption of lipopolysaccharide (LPS) and inflammation. To investigate whether RAGP ameliorated insulin resistance via effects on the gut microbiota, we performed 16s rDNA sequencing of ileal gut contents. This showed an amelioration of gut dysbiosis, with greater abundance of probiotic Lactobacillus and short-chain fatty acid-producing bacteria, and lower abundance of the Lachnospiraceae NK4A136 group and LPS-producing Desulfovibrio. The mechanism of the hypoglycemic effect of RAGP involves regulation of the gut microbiota, activation of the GLP-1/cAMP pathway to ameliorate insulin resistance. Thus, this study provides a theoretical basis for the use of RAGP to treat T2DM, and it may be a novel approach to restore the gut microbiota. |
| first_indexed | 2024-12-23T06:15:04Z |
| format | Article |
| id | doaj.art-2f52ac53a3f34478b182cc7051718091 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T06:15:04Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2f52ac53a3f34478b182cc70517180912022-12-21T17:57:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-06-011010.3389/fmicb.2019.01423456154A Purified Anthraquinone-Glycoside Preparation From Rhubarb Ameliorates Type 2 Diabetes Mellitus by Modulating the Gut Microbiota and Reducing InflammationHong-Xin Cui0Hong-Xin Cui1Ling-Shuai Zhang2Yang Luo3Ke Yuan4Zhi-Yong Huang5Ying Guo6College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, ChinaCollaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Zhengzhou, ChinaCollege of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, ChinaJiyang College of Zhejiang Agriculture and Forestry University, Zhuji, ChinaJiyang College of Zhejiang Agriculture and Forestry University, Zhuji, ChinaZhejiang Chinese Medical University, Hangzhou, ChinaZhejiang Chinese Medical University, Hangzhou, ChinaRheum palmatum L. is widely used in traditional Chinese medicine for the treatment of constipation. Here, the therapeutic effects and underlying mechanisms of purified anthraquinone-glycoside preparation from rhubarb (RAGP) on the type 2 diabetes mellitus (T2DM) rats were investigated. After 6 weeks of metformin and RAGP treatment, the weight returned to normal. Fasting blood glucose (FBG), glycated serum protein (GSP), insulin concentration and HOMA-IR index had significantly decreased, and glucagon-like peptide-1 (GLP-1) concentrations had increased. Histological abnormalities in the pancreas and ileum had improved. These effects were associated with enhanced intestinal integrity, thereby reducing the absorption of lipopolysaccharide (LPS) and inflammation. To investigate whether RAGP ameliorated insulin resistance via effects on the gut microbiota, we performed 16s rDNA sequencing of ileal gut contents. This showed an amelioration of gut dysbiosis, with greater abundance of probiotic Lactobacillus and short-chain fatty acid-producing bacteria, and lower abundance of the Lachnospiraceae NK4A136 group and LPS-producing Desulfovibrio. The mechanism of the hypoglycemic effect of RAGP involves regulation of the gut microbiota, activation of the GLP-1/cAMP pathway to ameliorate insulin resistance. Thus, this study provides a theoretical basis for the use of RAGP to treat T2DM, and it may be a novel approach to restore the gut microbiota.https://www.frontiersin.org/article/10.3389/fmicb.2019.01423/fullanthraquinone-glucosidediabetesgut microbiotaintestinal integrityinflammation |
| spellingShingle | Hong-Xin Cui Hong-Xin Cui Ling-Shuai Zhang Yang Luo Ke Yuan Zhi-Yong Huang Ying Guo A Purified Anthraquinone-Glycoside Preparation From Rhubarb Ameliorates Type 2 Diabetes Mellitus by Modulating the Gut Microbiota and Reducing Inflammation Frontiers in Microbiology anthraquinone-glucoside diabetes gut microbiota intestinal integrity inflammation |
| title | A Purified Anthraquinone-Glycoside Preparation From Rhubarb Ameliorates Type 2 Diabetes Mellitus by Modulating the Gut Microbiota and Reducing Inflammation |
| title_full | A Purified Anthraquinone-Glycoside Preparation From Rhubarb Ameliorates Type 2 Diabetes Mellitus by Modulating the Gut Microbiota and Reducing Inflammation |
| title_fullStr | A Purified Anthraquinone-Glycoside Preparation From Rhubarb Ameliorates Type 2 Diabetes Mellitus by Modulating the Gut Microbiota and Reducing Inflammation |
| title_full_unstemmed | A Purified Anthraquinone-Glycoside Preparation From Rhubarb Ameliorates Type 2 Diabetes Mellitus by Modulating the Gut Microbiota and Reducing Inflammation |
| title_short | A Purified Anthraquinone-Glycoside Preparation From Rhubarb Ameliorates Type 2 Diabetes Mellitus by Modulating the Gut Microbiota and Reducing Inflammation |
| title_sort | purified anthraquinone glycoside preparation from rhubarb ameliorates type 2 diabetes mellitus by modulating the gut microbiota and reducing inflammation |
| topic | anthraquinone-glucoside diabetes gut microbiota intestinal integrity inflammation |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01423/full |
| work_keys_str_mv | AT hongxincui apurifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT hongxincui apurifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT lingshuaizhang apurifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT yangluo apurifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT keyuan apurifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT zhiyonghuang apurifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT yingguo apurifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT hongxincui purifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT hongxincui purifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT lingshuaizhang purifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT yangluo purifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT keyuan purifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT zhiyonghuang purifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation AT yingguo purifiedanthraquinoneglycosidepreparationfromrhubarbamelioratestype2diabetesmellitusbymodulatingthegutmicrobiotaandreducinginflammation |