Detection of Antimicrobial Resistance, Pathogenicity, and Virulence Potentials of Non-Typhoidal <i>Salmonella</i> Isolates at the Yaounde Abattoir Using Whole-Genome Sequencing Technique
One of the crucial public health problems today is the emerging and re-emerging of multidrug-resistant (MDR) bacteria coupled with a decline in the development of new antimicrobials. Non-typhoidal <i>Salmonella</i> (NTS) is classified among the MDR pathogens of international concern. To...
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MDPI AG
2022-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/5/502 |
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| author | Chelea Matchawe Eunice M. Machuka Martina Kyallo Patrice Bonny Gerard Nkeunen Isaac Njaci Seraphine Nkie Esemu Dedan Githae John Juma Bawe M. Nfor Bonglaisin J. Nsawir Marco Galeotti Edi Piasentier Lucy M. Ndip Roger Pelle |
| author_facet | Chelea Matchawe Eunice M. Machuka Martina Kyallo Patrice Bonny Gerard Nkeunen Isaac Njaci Seraphine Nkie Esemu Dedan Githae John Juma Bawe M. Nfor Bonglaisin J. Nsawir Marco Galeotti Edi Piasentier Lucy M. Ndip Roger Pelle |
| author_sort | Chelea Matchawe |
| collection | DOAJ |
| description | One of the crucial public health problems today is the emerging and re-emerging of multidrug-resistant (MDR) bacteria coupled with a decline in the development of new antimicrobials. Non-typhoidal <i>Salmonella</i> (NTS) is classified among the MDR pathogens of international concern. To predict their MDR potentials, 23 assembled genomes of NTS from live cattle (n = 1), beef carcass (n = 19), butchers’ hands (n = 1) and beef processing environments (n = 2) isolated from 830 wet swabs at the Yaounde abattoir between December 2014 and November 2015 were explored using whole-genome sequencing. Phenotypically, while 22% (n = 5) of <i>Salmonella</i> isolates were streptomycin-resistant, 13% (n = 3) were MDR. Genotypically, all the <i>Salmonella</i> isolates possessed high MDR potentials against several classes of antibiotics including critically important drugs (carbapenems, third-generation cephalosporin and fluoroquinolone). Moreover, >31% of NTS exhibited resistance potentials to polymyxin, considered as the last resort drug. Additionally, ≤80% of isolates harbored “silent resistant genes” as a potential reservoir of drug resistance. Our isolates showed a high degree of pathogenicity and possessed key virulence factors to establish infection even in humans. Whole-genome sequencing unveiled both broader antimicrobial resistance (AMR) profiles and inference of pathogen characteristics. This study calls for the prudent use of antibiotics and constant monitoring of AMR of NTS. |
| first_indexed | 2024-03-10T03:12:12Z |
| format | Article |
| id | doaj.art-2f53471beb494dc390e89cf456dd2e23 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T03:12:12Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-2f53471beb494dc390e89cf456dd2e232023-11-23T12:31:37ZengMDPI AGPathogens2076-08172022-04-0111550210.3390/pathogens11050502Detection of Antimicrobial Resistance, Pathogenicity, and Virulence Potentials of Non-Typhoidal <i>Salmonella</i> Isolates at the Yaounde Abattoir Using Whole-Genome Sequencing TechniqueChelea Matchawe0Eunice M. Machuka1Martina Kyallo2Patrice Bonny3Gerard Nkeunen4Isaac Njaci5Seraphine Nkie Esemu6Dedan Githae7John Juma8Bawe M. Nfor9Bonglaisin J. Nsawir10Marco Galeotti11Edi Piasentier12Lucy M. Ndip13Roger Pelle14Institute of Medical Research and Medicinal Plants Studies, Yaounde P.O. Box 6163, CameroonBioscience Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi P.O. Box 30709, KenyaBioscience Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi P.O. Box 30709, KenyaInstitute of Medical Research and Medicinal Plants Studies, Yaounde P.O. Box 6163, CameroonDepartment of Biochemistry, University of Dschang, Dschang P.O. Box 96, CameroonBioscience Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi P.O. Box 30709, KenyaDepartment of Microbiology and Parasitology, University of Buea, Buea P.O. Box 63, CameroonBioscience Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi P.O. Box 30709, KenyaBioscience Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi P.O. Box 30709, KenyaDepartment of Rangeland, Animal Nutrition and Livestock Infrastructures, Sub Department of Animal Nutrition, Ministry of Livestock, Fisheries and Animal Industries (MINEPIA), Yaounde P.O. Box 930, CameroonInstitute of Medical Research and Medicinal Plants Studies, Yaounde P.O. Box 6163, CameroonDepartment of Agricultural, Food, Environment and Animal Sciences, University of Udine, 33100 Udine, ItalyDepartment of Agricultural, Food, Environment and Animal Sciences, University of Udine, 33100 Udine, ItalyDepartment of Microbiology and Parasitology, University of Buea, Buea P.O. Box 63, CameroonBioscience Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi P.O. Box 30709, KenyaOne of the crucial public health problems today is the emerging and re-emerging of multidrug-resistant (MDR) bacteria coupled with a decline in the development of new antimicrobials. Non-typhoidal <i>Salmonella</i> (NTS) is classified among the MDR pathogens of international concern. To predict their MDR potentials, 23 assembled genomes of NTS from live cattle (n = 1), beef carcass (n = 19), butchers’ hands (n = 1) and beef processing environments (n = 2) isolated from 830 wet swabs at the Yaounde abattoir between December 2014 and November 2015 were explored using whole-genome sequencing. Phenotypically, while 22% (n = 5) of <i>Salmonella</i> isolates were streptomycin-resistant, 13% (n = 3) were MDR. Genotypically, all the <i>Salmonella</i> isolates possessed high MDR potentials against several classes of antibiotics including critically important drugs (carbapenems, third-generation cephalosporin and fluoroquinolone). Moreover, >31% of NTS exhibited resistance potentials to polymyxin, considered as the last resort drug. Additionally, ≤80% of isolates harbored “silent resistant genes” as a potential reservoir of drug resistance. Our isolates showed a high degree of pathogenicity and possessed key virulence factors to establish infection even in humans. Whole-genome sequencing unveiled both broader antimicrobial resistance (AMR) profiles and inference of pathogen characteristics. This study calls for the prudent use of antibiotics and constant monitoring of AMR of NTS.https://www.mdpi.com/2076-0817/11/5/502multidrug-resistancewhole-genome sequencingnon-typhoidal <i>Salmonella</i>pathogenicity and virulencesilent resistant genesbeef carcass |
| spellingShingle | Chelea Matchawe Eunice M. Machuka Martina Kyallo Patrice Bonny Gerard Nkeunen Isaac Njaci Seraphine Nkie Esemu Dedan Githae John Juma Bawe M. Nfor Bonglaisin J. Nsawir Marco Galeotti Edi Piasentier Lucy M. Ndip Roger Pelle Detection of Antimicrobial Resistance, Pathogenicity, and Virulence Potentials of Non-Typhoidal <i>Salmonella</i> Isolates at the Yaounde Abattoir Using Whole-Genome Sequencing Technique Pathogens multidrug-resistance whole-genome sequencing non-typhoidal <i>Salmonella</i> pathogenicity and virulence silent resistant genes beef carcass |
| title | Detection of Antimicrobial Resistance, Pathogenicity, and Virulence Potentials of Non-Typhoidal <i>Salmonella</i> Isolates at the Yaounde Abattoir Using Whole-Genome Sequencing Technique |
| title_full | Detection of Antimicrobial Resistance, Pathogenicity, and Virulence Potentials of Non-Typhoidal <i>Salmonella</i> Isolates at the Yaounde Abattoir Using Whole-Genome Sequencing Technique |
| title_fullStr | Detection of Antimicrobial Resistance, Pathogenicity, and Virulence Potentials of Non-Typhoidal <i>Salmonella</i> Isolates at the Yaounde Abattoir Using Whole-Genome Sequencing Technique |
| title_full_unstemmed | Detection of Antimicrobial Resistance, Pathogenicity, and Virulence Potentials of Non-Typhoidal <i>Salmonella</i> Isolates at the Yaounde Abattoir Using Whole-Genome Sequencing Technique |
| title_short | Detection of Antimicrobial Resistance, Pathogenicity, and Virulence Potentials of Non-Typhoidal <i>Salmonella</i> Isolates at the Yaounde Abattoir Using Whole-Genome Sequencing Technique |
| title_sort | detection of antimicrobial resistance pathogenicity and virulence potentials of non typhoidal i salmonella i isolates at the yaounde abattoir using whole genome sequencing technique |
| topic | multidrug-resistance whole-genome sequencing non-typhoidal <i>Salmonella</i> pathogenicity and virulence silent resistant genes beef carcass |
| url | https://www.mdpi.com/2076-0817/11/5/502 |
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